Báo cáo khoa học: " Characterization of subcellular localization of duck enteritis virus UL51 protein" pptx

... position of the pUL51 (about 34 kDa). BioMed Central Page 1 of 8 (page number not for citation purposes) Virology Journal Open Access Research Characterization of subcellular localization of duck enteritis ... study the locations of the DEV pUL51. Intracellular localization and distribution of DEV pUL51 in DEV-infected cells A detailed analysis of the intracellular...

Ngày tải lên: 12/08/2014, 04:22

... and study of the genetic organization of the exe gene cluster of Aeromonas salmonicida. Gene 158, 77–82. 30 Martin PR, Hobbs M, Free PD, Jeske Y & Mattick JS (1993) Characterization of pilQ, ... charac- terization of seven distinct competence gene loci in the genome of T. thermophilus HB27 comprising a total of 16 potential genes of the DNA translocator [6–8]. However, s...

Ngày tải lên: 07/03/2014, 12:20

... Amplification of cDNA templates with a set of primers corresponding to the Xenopus EF1a elongation factor cDNA (280 bp) as internal control of the poly(A) + RNA. (C) Tissue comparison of the level of expression ... Biochem. 269) Ó FEBS 2002 Characterization and functional expression of cDNAs encoding thyrotropin-releasing hormone receptor from Xenopus laevis Identification of a...

Ngày tải lên: 21/02/2014, 03:20

... stock virus. For virus amplification, Sf9 cells were infected with the stock virus, and supernatant was collected then virus concentra- tion was determined by plaque forming assay. SDS/PAGE The virus- infected ... was induced by clofibrate, but the level of induction was lower than that of EH activity. Cloning of a DmEH gene Based on its substrate selectivity and localization, the...

Ngày tải lên: 07/03/2014, 21:20

... the addition of 1 lgÆmL )1 of tetracycline to the medium. TAP purification was performed as described elsewhere [43]. Digitonin fractionation and subcellular localization Purification of mitochondrial ... (PHB1). Localization of SCL protein We used a tagging strategy to analyze the intracellu- lar localization of this protein. A hemagglutinin (HA 3 ) tag was attached to the C-...

Ngày tải lên: 22/03/2014, 21:20

... confirming the importance of these three resi- dues for the activity of EstD. Discussion In this contribution the cloning, expression, and char- acterization of a new type of esterase from the hyper- thermophilic ... 3. 3D model of EstD. (A) The overall structure of the C-ter- minal domain of EstD. The central b-sheet and surrounding a-helixes are shown in black and grey, respect...

Ngày tải lên: 23/03/2014, 09:20

... and characterization of benzonitrilases from Arthrobacter sp. strain J -1. Appl. Environ. Microbiol. 51, 302±306. 25. Harper, D.B. (1977) Fungal degradation of aromatic nitriles. Enzymology of ... production of lactams from aliphatic a,x-dinitriles. J. Org. Chem. 63, 4792±4801. 32. Goldlust, A. & Bohak, Z. (1989) Induction, puri®cation, and characterization of the nitrilase...

Ngày tải lên: 23/03/2014, 21:21

... p.p.m. consistent with the presence of a substituent at the 7-position of HepII. Signals indicative of the presence and absence of substituents at the 7-position of HepII in the L4 galE oligosaccharide, ... p.p.m. which is characteristic for substitution of the 6-position of Fig. 3. Regions of the (A) 2D- 13 C- 1 H-HSQC NMR and (B) 2D- 13 C- 1 H-HMBC NMR spectra of the core...

Ngày tải lên: 23/03/2014, 21:21

... regulation of cardosin B gene expression. Comparison of the leader intron of the cardosin B gene with pistil-specific genes revealed the presence of putative regulatory elements. A region of SLG 13 ,a gene ... region of the cardosin A gene allowed the identification of a region of approximately 500 bp essential for gene expression in transgenic flowers. Additionally, the relevanc...

Ngày tải lên: 30/03/2014, 09:20

... FEBS 2001 level of fetal and adult liver rats. The results show a new localization of BCATm in the nuclei of fetal hepatocytes and the presence of an active and inactive form of the BCATm in fetal ... addition of 500 mLof2 M sodium acetate pH 3.4. The remaining 2-oxo[1- 14 C]isocaproate not transaminated was chemically decarboxylated by adding 250 mL of 30% hydrogen peroxide....

Ngày tải lên: 31/03/2014, 23:20