Báo cáo khoa học: "The complete genome of klassevirus – a novel picornavirus in pediatric stool" doc

... The 5' UTR ends with two in- frame AUG A. Genome organization of human klassevirus 1Figure 1 A. Genome organization of human klassevirus 1. Conserved picornaviral domains present in klassevirus ... kv761R 5'-CAG CCA ACG AAC TCG AAA AT-3'. The 761 bp amplicon was gel purified and cloned using TOPO TA cloning kit (Invitrogen). TOPO TA plas- mid containing the 7...

Ngày tải lên: 12/08/2014, 04:22

... of California, San Francisco, California 94143, USA, 2 Departments of Laboratory Medicine and Medicine, Division of Infectious Diseases, University of California, San Francisco, California 94143, ... the same adapter- linked oligo-dT primer. 454 Pyrosequencing A total of 141 amplified cDNA libraries that were negative by array and PCR were cleaned via Ampure beads (Agen- court)...

Ngày tải lên: 12/08/2014, 04:21

... insertion of endotra- cheal tubes, intravenous lines and chest drains, but not after insertion of nasogastric tubes. In addition, CXRs were obtained in the case of worsening of oxygenation. As ... clinically relevant and irrelevant findings, and simply reported on all abnormalities [12]. At present, in many ICUs CXRs are still routinely obtained on a daily basis, at least in...

Ngày tải lên: 25/10/2012, 10:39

... other activating factors. The results of employing plasmid-based ChIP assays on the Cdc2 promoter indicate that E2F3 binding to the distal acti- vating E2F site may require an intact CCAAT-box occupied ... 1). Cyclins B1 and B2 appear in S phase and accumulate in G 2 and mitosis before disappearing at the transition from metaphase to anaphase. Synthe- sis is controlled at the level of...

Ngày tải lên: 16/02/2014, 09:20

... life-threatening complications of cerebral malaria, and as an important tool in validat- ing our proposal of hACMSD as a novel drug target for the treatment of diabetes and to investigate its proposed ... Regulation of mouse hepatic a- amino-b-carbo- xymuconate-e-semialdehyde decarboxylase, a key enzyme in the tryptophan–nicotinamide adenine dinu- cleotide pathway, by hepat...

Ngày tải lên: 18/02/2014, 06:20

... 2 0–2 94) were 5¢-aataatccatggggagtaatacttcctggcgta aaagtgaagtcc-3¢ and 5¢-attattggatccctattgctggtccgattctgccag-3¢. 3-TPR NlpI primers (residues 6 2–1 97) were 5¢-aataatccatgg gggcacagcttttatatgagcgcggag-3¢ ... structures have been determined share a common tertiary organ- ization, which is dominated by interactions between A helices and the preceding AB pair (Fig. 1A) . Local AB, BA¢...

Ngày tải lên: 07/03/2014, 16:20

... W .A. (1996) Molecular determinants of high a nity binding of alpha- scorpion toxin and sea anemone toxin in the S3–S4 extracellular loop in domain IV of the Na + channel alpha subunit. J. Biol. Chem. ... rectangle the slower the exchange. Arrows indicate b-strands and zig-zag lines indicate the a- helix. (B) Ribbon diagram of Cn12 showing an a- helix at residues 2 4–3 2, th...

Ngày tải lên: 23/03/2014, 12:20

... by phytochrome and a plastid signal during de-etiolation in Arabidopsis thaliana. Plant J 25, 54 9–5 61. 54 Sambrook J & Russell DW (2001) Molecular Cloning – a Laboratory Manual. Cold Spring Harbor Laboratory Press, ... becomes a Val or Leu in mammalian and other animal ⁄ bacterial sequences, respectively, Ile214 is a Leu in cyanobacterial and ani- mal HOs, Tyr231 is...

Ngày tải lên: 30/03/2014, 11:20

... telomerase activity, induction of reactive oxygen species release, and inhibi- tion of survival pathways involving extracellular signal regulated kinase, Akt, calcium signaling and NF-κB activ- ities ... conformational change, Bax acti- vation and up-regulation of total Bax expression involved arsenic trioxide-induced apoptosis rather than affecting total Bcl-2 expression in human...

Ngày tải lên: 09/08/2014, 03:21

... d Harwich (P). In North Africa, Europe and Asia the distribution patterns change dramatically, P strains being almost absent and M strains very common. In fact, a marked ... other main area where P strains has been found is Central Africa from Senegal to Kenya. In these regions M strains also appear rare and the observation of a rela...

Ngày tải lên: 09/08/2014, 22:23