Báo cáo khoa học: " Development of TaqMan® MGB fluorescent real-time PCR assay for the detection of anatid herpesvirus 1" pdf

Báo cáo khoa học: Afﬁnity puriﬁcation-mass spectrometry Powerful tools for the characterization of protein complexes ppt

... afﬁnity-based methods. The commontheme of these is the use of an inherent interaction (afﬁnity) of two biomolecules. If one of the molecules is immobilizedon a solid support, the interacting molecule ... and that the results areusually straightforward to interpret. The downsides are the statistical limitations imposed by the method. Theseinclude the requirement that the majority of the codingsequence ... in the sequence (Fig. 2). Quite often,only a part of the sequence can be read from the sequence.However, this stretch of consecutive sequence is ÔlockedÕwithin the peptide by the masses of the...

Báo cáo khoa học: "Improvement of a real-time RT-PCR assay for the detection of enterovirus RNA" ppt

... validation of a new real-time PCR assay for detection of enteroviruses and parechoviruses, and implica-tions for diagnostic procedures. Journal of Clinical Virology 2008,41:75-80.Table 3: Limit of detection ... WA, Bruynseels P, Mertens AH: Evaluation of a rapid real-time RT -PCR assay for the detection of enterovirusRNA in cerebrospinal fluid specimens. Journal of Clinical Virology2002, 25:S39-S43.7. ... beuseful in the case of equivocal results. Further investiga-tion however should be performed on the detection of Coxsackievirus A24.Competing interests The authors declare that they have no...

Báo cáo khoa học: "Improvement of a real-time RT-PCR assay for the detection of enterovirus RNA" doc

... includ-ing different RT -PCR assays [3,4].Our study group earlier reported a real-time RT -PCR assay for the detection of enterovirus RNA, based on the 5' exo-nuclease digestion of a dual-labeled ... validation of a new real-time PCR assay for detection of enteroviruses and parechoviruses, and implica-tions for diagnostic procedures. Journal of Clinical Virology 2008,41:75-80.Table 3: Limit of detection ... BioMed CentralPage 1 of 3(page number not for citation purposes)Virology JournalOpen AccessShort reportImprovement of a real-time RT -PCR assay for the detection of enterovirus RNAMarian...

Báo cáo khoa học: "Acetazolamide-mediated decrease in strong ion difference accounts for the correction of metabolic alkalosis in critically ill patients" pps

... inhibition of carbonic anhy-drase in the luminal borders of renal proximal tubule cells, andit is often effectively used in the treatment of metabolic alkalo-sis in the ICU. However, the mechanism of ... dose of acetazolamide is an effective andsafe form of therapy, with a quick onset and long duration of action [5,15]. Our findings suggest that the duration of the pharmacologic effect of a ... methodsPatients The local ethics committee granted approval for the study and,because the indication for acetazolamide was based on clini-cal grounds, waived the need for informed consent....

Báo cáo hóa học: " A quantum dots and superparamagnetic nanoparticle-based method for the detection of HPV DNA" ppt

... 9 of 9specific PCR, the greatest advantages of our QDs andsuperparamagnetic nanoparticle-based hybridizationconsistsinthetimeofdetectionandeaseofprocess.Generally speaking, type-specific PCR ... method for the detection of HPV DNAWang Yu-Hong1†, Chen Rui2†and Li Ding3*AbstractBackground: The recent advance in nanomaterial research field prompts the development of diagnostics of infectious ... hybridization assay for the detection of (HPV) 16 infections which combines the merits of superparamagnetic nanoparticles and QDs andwholly differs from a conventional hybridization assay at that the...

báo cáo hóa học:" Research Article Linear Classiﬁer with Reject Option for the Detection of Vocal Fold Paralysis and Vocal Fold Edema" doc

... suggests that although the probability of rejection is a fraction of the test samples, the probability of false alarm and the probability of detection is now a fraction of the test samples, which ... yieldsprobability of detection approximately 73% for probability of false alarm 4% [33]. For the same probability of falsealarm, a probability of detection, which falls between 80.95% for rectangular ... isderived. The optimal operating point of the linear classiﬁeris speciﬁed with and without reject option. The contribution of this paper is in the assessment of the impact of rejectoption in the ROC...

Báo cáo khoa học:" Development of TaqMan® MGB fluorescent real-time PCR assay for the detection of anatid herpesvirus 1?" pdf

... citation purposes)Virology JournalOpen AccessResearch Development of TaqMan® MGB fluorescent real-time PCR assay for the detection of anatid herpesvirus 1Yufei Guo1,2, Anchun Cheng*1,2, Mingshu ... fragments (498 bp) on the agarose gel. The detection limit of the FQ -PCR was deter-mined based on the highest dilution that resulted in the presence of CT value in real-time PCR detection. DNA from ... http://www.virologyj.com/content/6/1/71Page 3 of 8(page number not for citation purposes)Establishment of the fluorescent quantitative real-time PCR standard curveFigure 1Establishment of the fluorescent quantitative real-time PCR...

Báo cáo khoa học: " Development of TaqMan® MGB fluorescent real-time PCR assay for the detection of anatid herpesvirus 1" pdf

... CentralPage 1 of 8(page number not for citation purposes)Virology JournalOpen AccessResearch Development of TaqMan® MGB fluorescent real-time PCR assay for the detection of anatid herpesvirus ... we describe a fluorescent quantitative real-time PCR (FQ -PCR) method developed for fast measurement of AHV-1 DNA based on TaqMan MGB technology.Results: The detection limit of the assay was 1 ... http://www.virologyj.com/content/6/1/71Page 3 of 8(page number not for citation purposes)Establishment of the fluorescent quantitative real-time PCR standard curveFigure 1Establishment of the fluorescent quantitative real-time PCR...

Tài liệu Báo cáo khoa học: What makes biochemical networks tick? A graphical tool for the identiﬁcation of oscillophores ppt

... each other). The value (the sign) of a cycle is the product of the values (the signs) of its steps. The sign o f the magnitude of a Ôminus combined autoinﬂuence path of order pÕ equal those of the ... subgraphs of the same order in the same network, as was illustrated for the two examples inthis paper. The network outside the former s ubgraphmay do away with the oscillophoretic potential of the subgraph ... concentrations of itsspecies. Therewith the c oefﬁcient of o rder p of the characteristic equation equals the sum of all simplestcombined Ôminus autoinﬂuence paths of order pÕ in the network....

Báo cáo khoa học: Ionizing radiation utilizes c-Jun N-terminal kinase for ampliﬁcation of mitochondrial apoptotic cell death in human cervical cancer cells pptx

... treatment. The mitochondrial transmembrane potential of these cells was determined by assaying the retention of DioC6(3) added during the last 30 min of treatment. After removal of the medium, the ... protein of the Bcl-2 family, in the presence of apoptotic stimuli. The truncated Bid then triggers acti-vation of Bax and ⁄ or Bak and mitochondrial release of pro-apototic molecules into the cytosol ... with 10 Gy of c-radiation. After 72 h, the mitochondrial trans-membrane potential of these cells was determined by assaying the retention of DioC6(3) added during the last 30 min of treatment....

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