Báo cáo khoa học: " Development of TaqMan® MGB fluorescent real-time PCR assay for the detection of anatid herpesvirus 1" pdf

... affinity-based methods. The common theme of these is the use of an inherent interaction (affinity) of two biomolecules. If one of the molecules is immobilized on a solid support, the interacting molecule ... and that the results are usually straightforward to interpret. The downsides are the statistical limitations imposed by the method. These include the requirement t...

Ngày tải lên: 31/03/2014, 07:20

... validation of a new real-time PCR assay for detection of enteroviruses and parechoviruses, and implica- tions for diagnostic procedures. Journal of Clinical Virology 2008, 41:75-80. Table 3: Limit of detection ... WA, Bruynseels P, Mertens AH: Evaluation of a rapid real-time RT -PCR assay for the detection of enterovirus RNA in cerebrospinal fluid specimen...

Ngày tải lên: 12/08/2014, 04:21

... includ- ing different RT -PCR assays [3,4]. Our study group earlier reported a real-time RT -PCR assay for the detection of enterovirus RNA, based on the 5' exo- nuclease digestion of a dual-labeled ... validation of a new real-time PCR assay for detection of enteroviruses and parechoviruses, and implica- tions for diagnostic procedures. Journal of Cli...

Ngày tải lên: 12/08/2014, 04:22

... inhibition of carbonic anhy- drase in the luminal borders of renal proximal tubule cells, and it is often effectively used in the treatment of metabolic alkalo- sis in the ICU. However, the mechanism of ... dose of acetazolamide is an effective and safe form of therapy, with a quick onset and long duration of action [5,15]. Our findings suggest that the duration of...

Ngày tải lên: 12/08/2014, 23:21

... 9 of 9 specific PCR, the greatest advantages of our QDs and superparamagnetic nanoparticle-based hybridization consistsinthetimeofdetectionandeaseofprocess. Generally speaking, type-specific PCR ... method for the detection of HPV DNA Wang Yu-Hong 1† , Chen Rui 2† and Li Ding 3* Abstract Background: The recent advance in nanomaterial research field prompts the developmen...

Ngày tải lên: 21/06/2014, 01:20

... suggests that although the probability of rejection is a fraction of the test samples, the probability of false alarm and the probability of detection is now a fraction of the test samples, which ... yields probability of detection approximately 73% for probability of false alarm 4% [33]. For the same probability of false alarm, a probability of detectio...

Ngày tải lên: 21/06/2014, 20:20

... citation purposes) Virology Journal Open Access Research Development of TaqMan ® MGB fluorescent real-time PCR assay for the detection of anatid herpesvirus 1 Yufei Guo 1,2 , Anchun Cheng* 1,2 , Mingshu ... fragments (498 bp) on the agarose gel. The detection limit of the FQ -PCR was deter- mined based on the highest dilution that resulted in the prese...

Ngày tải lên: 12/08/2014, 04:21

... Central Page 1 of 8 (page number not for citation purposes) Virology Journal Open Access Research Development of TaqMan ® MGB fluorescent real-time PCR assay for the detection of anatid herpesvirus ... we describe a fluorescent quantitative real-time PCR (FQ -PCR) method developed for fast measurement of AHV-1 DNA based on TaqMan MGB technology. Resul...

Ngày tải lên: 12/08/2014, 04:21

... each other). The value (the sign) of a cycle is the product of the values (the signs) of its steps. The sign o f the magnitude of a Ôminus combined autoinfluence path of order pÕ equal those of the ... subgraphs of the same order in the same network, as was illustrated for the two examples in this paper. The network outside the former s ubgraph may do aw...

Ngày tải lên: 19/02/2014, 16:20

... treatment. The mitochondrial transmembrane potential of these cells was determined by assaying the retention of DioC 6 (3) added during the last 30 min of treatment. After removal of the medium, the ... protein of the Bcl-2 family, in the presence of apoptotic stimuli. The truncated Bid then triggers acti- vation of Bax and ⁄ or Bak and mitochondrial release of...

Ngày tải lên: 07/03/2014, 05:20