... Pan et al.(Eur. J. Biochem. 271) Ó FEBS 2004 Trehalose synthase of Mycobacterium smegmatis Purification, cloning, expression, and properties of the enzyme Yuan T. Pan1, Vineetha Koroth Edavana1, ... catalytic mechanism of TreS, each of the r adioactive substrates was incubated with the enzyme in the presence of high concentrations of the unlabeledproduct, and the formation of radioactive product ... each other. In fact, asfree glucose appears to be one of the products of the purified enzyme activity, and radioactive glucose, in the presence of maltose or trehalose can be converted by the enzyme...