... of the mutations AP -2 ⁄ ) 21 3, AP -2 ⁄ ) 166 and Pax-3 ⁄ ) 113, labeled CRMP- 2 )22 9 ⁄ ) 151 or Pax-3 ⁄ ) 113M oligonucleotides were tried in EMSA using the mutated sequences AP -2 ⁄ ) 21 3M, AP -2 ... characterization of the regulatory region of the human CRMP -2 gene As a second step in our study, we aimed to evaluate the presence of regulatory consensus sequ...
Ngày tải lên: 16/03/2014, 12:20
... 140 32 Caen cedex, France Fax: +33 23 1565346 Tel: +33 23 1565361 E-mail: pascal.favrel@unicaen.fr (Received 22 February 20 07, revised 10 May 20 07, accepted 23 May 20 07) doi:10.1111/j.17 42- 4658 .20 07.05898.x Chitinase-like ... leading to the identification of the two first C. gigas members of the GH18 family, Cg-Clp1 (AJ97 124 1) [5] and the chi- tinase Cg-Chit (AJ97 1...
Ngày tải lên: 19/02/2014, 00:20
Tài liệu Báo cáo khoa học: Characterization of human deoxyribonuclease I gene (DNASE1) promoters reveals the utilization of two transcription-starting exons and the involvement of Sp1 in its transcriptional regulation ppt
... RT-PCR, the locations of the primers DN)105 and DN)9 used in ChIP assays, the locations of the primers DN +23 2 and DN +25 4 utilized in 5¢-RACE, and the locations of the primers DN+385 and DN+ 721 used ... numbers over the diagrams indicate the position of the corresponding nucleotides relative to the translation start site, and the numbers in parentheses below...
Ngày tải lên: 19/02/2014, 06:20
Tài liệu Báo cáo khoa học: Characterization of the interaction between the plasma membrane H+-ATPase of Arabidopsis thaliana and a novel interactor (PPI1) doc
... 14-3-3s. Results The C-terminus of isoform 1 of the PM H + -ATPase of A. thaliana (AHA1) interacts with the first 88 amino acids of PPI1 [22 ], indicating that the PM H + -ATPase binding site of PPI1 is ... study the mechanism of action of PPI1. The analysis of the pH dependence of PPI1- induced activation of the H + -ATPase showed that stimulation decreases...
Ngày tải lên: 19/02/2014, 07:20
Tài liệu Báo cáo khóa học: Characterization of the products of the genes SNO1 and SNZ1 involved in pyridoxine synthesis in Saccharomyces cerevisiae pptx
... min, the absorbance of the supernatant was read at 363 nm. The absorbance of the reduced form of APAD (APADH) was linear over the 2 100 l M range of glutamate with a molar extinction coefficient of ... capable of complementing the defect of mutant K64 (Figs 1 and 2) . They are homologues of PDX2 and PDX1 of fungus C. nicotianae, respectively [7 ,20 ], strongly s...
Ngày tải lên: 19/02/2014, 12:20
Tài liệu Báo cáo khoa học: Characterization of promoter 3 of the human thromboxane A2 receptor gene A functional AP-1 and octamer motif are required for basal promoter activity docx
... (RLU) * * * * -1394 AP-1 ( -27 ) Oct 1 (- 123 ) Oct 1 /2 (-105) -404 - 320 +1 -1394-1394 AP-1 ( -27 ) Oct 1 (- 123 ) Oct 1 /2 (-105) -404 - 320 +1 AP-1 ( -27 ) Oct 1 (- 123 ) Oct 1 /2 (-105) -404 - 320 - 320 A B +1+1 024 6810 12 Lucife ... Luc -404 +1 Luc - 320 +1 Luc +1 Luc - 320 +1 Luc - 320 +1 Luc+1 Luc - 320 +1 Luc - 320 +1 Luc +1 Luc - 320 AP-1 ( -27 ) Oct1 (- 123 ) Oct1 /...
Ngày tải lên: 19/02/2014, 16:20
Tài liệu Báo cáo khoa học: Characterization and mode of action of an exopolygalacturonase from the hyperthermophilic bacterium Thermotoga maritima doc
... (GalpA) 2 (Table 1). Subsite mapping On the basis of the assumptions of Hiromi [30] that the intrinsic rate of hydrolysis (k int ) in the productive complex is independent of the length of the substrate, K m and ... L. Kluskens et al. 54 72 FEBS Journal 27 2 (20 05) 5464–5473 ª 20 05 FEBS Characterization and mode of action of an exopolygalacturonase from the...
Ngày tải lên: 20/02/2014, 03:20
Tài liệu Báo cáo khoa học: Characterization of ICAM-4 binding to the I domains of the CD11a/CD18 and CD11b/CD18 leukocyte integrins pptx
... Tris buffer, and then resuspended in the Tris buffer containing either 2 m M MgCl 2 and 2 m M CaCl 2 ,2m M MgCl 2 ,2m M CaCl 2 ,2m M MnCl 2 ,or 4m M EDTA. Plastic wells precoated with 0 .2 lg of anti-GST ... binding of any of the ICAM transfectants to the I domain fusion proteins. Figure 9 shows that the presence of Mg 2+ and Ca 2+ is needed for efficient adhes...
Ngày tải lên: 20/02/2014, 11:20
Tài liệu Báo cáo khoa học: Characterization of the promoter for the mouse a3 integrin gene Involvement of the Ets-family of transcription factors in the promoter activity doc
... in the DDBJ/EMBL/ GenBank databases under the accession number AB08 022 9 (Received 1 May 20 02, revised 19 July 20 02, accepted 26 July 20 02) Eur. J. Biochem. 26 9, 4 524 –45 32 (20 02) Ó FEBS 20 02 doi:10.1046/j.14 32- 1033 .20 02. 03146.x To ... FEBS 20 02 Integrin a3 gene promoter (Eur. J. Biochem. 26 9) 4 527 Characterization of the promoter for the mouse a3 inte...
Ngày tải lên: 21/02/2014, 03:20
Báo cáo khoa học: Characterization of the rice carotenoid cleavage dioxygenase 1 reveals a novel route for geranial biosynthesis ppt
... 5A). The relative amount (about 7%) of the C 20 dialdehyde was much higher than of the C 22 dialde- hyde (about 2% ), indicating a higher affinity for the novel C7–C8 double bond than for the C5–C6 ... solution of the HPLC-purified compound, and then incubating for 20 min at 50 °C. The product was then par- titioned against petroleum ether ⁄ diethyl ether 1 : 4 (v ⁄ v)...
Ngày tải lên: 07/03/2014, 03:20