Báo cáo khoa học: "Dengue viruses binding proteins from Aedes aegypti and Aedes polynesiensis salivary glands" ppt

... monoclonal antibodiesFigure 2 DENV -binding proteins from Ae aegypti salivary glands detected with anti-E monoclonal antibodies. Total proteins from Ae aegypti salivary gland extracts were treated ... polyclonal antibodiesFigure 1 DENV -binding proteins from Ae aegypti salivary glands detected with anti-DENV polyclonal antibod- ies. Total proteins from Ae ae...

Ngày tải lên: 12/08/2014, 04:21

... lgÆmL )1 )wasused. Proteins, GAGs, radiolabelling and binding assay Recombinant protein H, M1 protein and the A-S and S-C3 fragments of M1 protein were prepared as described [23,31]. Protein SIC was purified from ... Polyclonal human IgG, albumin and fibrinogen were purchased from Sigma. Chondroitinase ABC (EC 4.2.2.4) was purchased from ICN and heparan sulfate lyase (EC 4.2....

Ngày tải lên: 20/02/2014, 11:20

... HCR ⁄ C and HCR ⁄ D-SA, HCR ⁄ D did not show detectable cell binding, suggesting that HCR ⁄ D binding and affinity varied between HCR ⁄ C and HCR ⁄ D-SA. Under simi- lar conditions, HCR ⁄ A binding ... HCR ⁄ C and HCR ⁄ D-SA. Thus, HCR ⁄ C and HCR ⁄ D-SA utilize the GBL for ganglioside binding. The GBLs of BoNT ⁄ C, BoNT ⁄ D and BoNT ⁄ D-SA represent a gain of function of ga...

Ngày tải lên: 22/03/2014, 15:21

... C-rich strands, we performed CD stud- ies (Fig. S3) and UV melting studies at 287 and 265 nm (Fig. S4) at both pH 6.0 and 7.0. Both CD and UV melting studies showed that the C-rich strands c-kitC0, c-kitC4 ... c-kitC6, c-kitC8 and c-kitC12 sequences at pH 6.0 and 7.0. Fig. S4. Normalized UV annealing and melting curves for c-kitC0, c-kitC4, c-kitC6, c-kitC8 and c-kitC12 comple...

Ngày tải lên: 07/03/2014, 02:20

... in nat- ure, and is responsible for the critical processes of light capture, electron transport and photophosphorylation. Most of the resident proteins are imported from the cytosol and then transported ... TatA, TatB and TatC, respec- tively. Tha4 and Hcf106 are single-span membrane proteins containing an N-terminal transmembrane domain followed by a short amphipathic helical...

Ngày tải lên: 07/03/2014, 03:20

... specific proteins in the hemolymph, namely LPS -binding proteins, b-glucan b inding proteins [2], or lectins [3]. b-1,3-Glucan binding proteins have been identified in Penaeus californiensis and in ... obtained sequences. The lower band yielded peptides similar to histone H4, the upper b ands contained peptides from histones H2A and H2B, and the highest molecular mass band pro...

Ngày tải lên: 07/03/2014, 16:20

... E. coli of two plant GPXs, GPXle1 and GPXha2, from Lycopersicon esculentum and Helianthus annuus, respect- ively. The purified recombinant proteins were obtained and used in enzymatic assays with ... hydroperoxide GPX (GPX4) and selenoindependent epididymis GPX (GPX5) [7,8]. To date, cDNAs encoding proteins similar to animal GPX have been isolated from different plants and ha...

Ngày tải lên: 08/03/2014, 22:20

... genera- tion and oxidative stress with ageing and exercise: dif- ferential effects in the myocardium and liver. Acta Physiol Scand 169, 343–351. 8 Møller P & Loft S (2006) Dietary antioxidants and ... produced 82.0 ± 2.6 lm H 2 O 2 from PMA-stimulated PMNs. At 100 lm, selenourea A and selenourea B produced 8.3 ± 5.2 lm and 11.7 ± 1.7 lm H 2 O 2 , respectively, and selenoam...

Ngày tải lên: 23/03/2014, 07:20

... Mg 2+ . ADP and GDP inhibit the B. caldo- lyticus enzyme in a cooperative fashion with Hill coefficients of 2.9 for ADP and 2.6 for GDP. K i values are determined as 113 and 490 l M for ADP and GDP, ... phospho- ribosylpyrophosphate synthetase and crystallization and pre- liminary x-ray characterization of t he free enzyme and its substrate-effector complex. Proteins 24 , 238 –24...

Ngày tải lên: 23/03/2014, 13:20

... di di prep.simple 2 andare vad dv 1 _andare v.intran.simple 1 andare and I dv2. andar© v.intran.simple I The other sets of data are contained in the Prolog workspace and are structured as ... chaining the prefix with the original word. For example, from the verb to handle with the prefix re we obtain the verb to rehandle. Morphologlc and syntactic characteristics remain the...

Ngày tải lên: 24/03/2014, 05:21