Báo cáo khoa học: "Isolation and characterization of a genotype 4 Hepatitis E virus strain from an infant in China" ppt
Báo cáo khoa học: "Isolation and characterization of a genotype 4 Hepatitis E virus strain from an infant in China" ppt
... Rayo CD, Bezanilla JC, Toth TE, MengXJ: Identification of genotype 3 hepatitis E virus (HEV) in serum and faecal samples from pigs in Thailand and Mexico,where genotype 1 and 2 HEV strains are ... with another Shanghai HEV strain (AB1976 74 ) isolated from a Japanese patient who wasreportedly infected during traveling in Shanghai of China[8]. Phylogenetic analysis indicated that the isolate ... 236 infants younger than 2 year old in ShanghaiChildren's Hospital, these infants were sent to the hospi-tal because they had abnormal feces appearance for sev-eral days. All the samples...
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Tài liệu Báo cáo khoa học: Isolation and characterization of four type 2 ribosome inactivating pulchellin isoforms from Abrus pulchellus seeds docx
... havebeen found in the Caryophyllaceae, Sambucaceae,Cucurbitaceae, Euphorbiaceae, Phytolaccaceae and Poaceae [1]. Although many are potentially useful in agriculture and medicine because of their ... bodymass. The mice had free access to food and water and testswere carried out over 48 h. The results were evaluated asthe median lethal dose. Each assay had an animal controlthat received only ... likely cleaved after an Asn residue once the protein is deposited in vacu-oles. The intervening linker peptides are also extremelysimilar and, by analogy to that of preproricin, may wellcontain...
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Báo cáo khoa học: Isolation and characterization of a D-cysteine desulfhydrase protein from Arabidopsis thaliana pptx
... the amino group forming the iminelinkage with the coenzyme. Enzymes of the b-familycatalyse mainly b-replacement or b-elimination reac-tions. The d-alanine aminotransferase and the alanineracemase ... ground wasused for the analyses. (A) Total RNA was extracted and 20 lg RNAwas loaded in each lane and blotted as indicated in Experimentalprocedures. To prove equal loading of the extracted RNA ... synthaseactivities were determined as described [20].Other proceduresProtein contents were determined according to [56] usingBSA as a protein standard. The DNA and amino acidsequence analyses and prediction...
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Báo cáo khoa học: Isolation and characterization of an IgNAR variable domain specific for the human mitochondrial translocase receptor Tom70 potx
... were modifiedby alkylation, or replaced with alanine and valine, respect-ively, and the resultant VNARs evaluated for retention of binding affinity.Material and methodsEquipment and reagentsRestriction ... (12F-11DCys). Use of alanine and valine was initially determined in a set of competitiveselection experiments [35], and replacement with this pair of residues maintains a hydrophobic character suitable foramino ... wereperformed with an increase in the stringency of washing ateach step, and between selection rounds three and four a significant ( 1000-fold) increase in the titre of elutedbacteriophage was...
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Báo cáo khóa học: Isolation and characterization of the Xenopus HIVEP gene family ppt
... upstream of this motif. The isolated pool was also enriched in sequenceshaving a GAGA or GACCG. These motifs were oftenoverlapping and the GAGA and GACCG sequences werelocated with a variable ... consisting of Mad/Medea and Shn. While the fundamental aspects of TGF-b signaling are highly conserved and the requirement of this pathway in embryonic patterning in both inverte-brates and vertebrates ... gel and used in successive rounds of amplification and selection.After seven rounds, the selected DNA duplexes weresubcloned and 49 clones were sequenced (Fig. 6A) . In all of the clones analyzed,...
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Báo cáo khoa học: " Isolation and characterization of canine umbilical cord blood-derived mesenchymal stem cells" pps
... based therapies and tissue engineering which have been performed in trials to overcome the difficulties of gene based therapies and their medical limitations. The use of stem cell implantation ... or medical trials for preclinical purposes [23,30]. One advantage of using dogs is that canine model transplantation uses a large size animal [9]. The isolation and characterization of CD 34+ ... blood and maintaining cell culture for experimental analysis. A guarantee of enough numbers of multipotent stem cells out of a very small quantity of cord blood sample from the canine umbilical...
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Báo cáo khoa học: "Isolation and characterization of Treponema phagedenis-like spirochetes from digital dermatitis lesions in Swedish dairy cattle" pps
... DQ470656, EF05 741 1, EU375 741 – EU375 744 and EU41 048 4.BioMed CentralPage 1 of 8(page number not for citation purposes)Acta Veterinaria ScandinavicaOpen AccessResearchIsolation and characterization ... treat DD, take preventive measures and startcontrol programmes the cause/s of the disease needs to beidentified and characterized. We describe seven isolates of a Treponema sp. from cattle ... hasbeen reported.More than one phylotype of Treponema can be present in the same lesion. Different phylotypes have been isolated from the same animal [8,16] and by cloning and sequenc-ing of...
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Tài liệu Báo cáo khoa học: Production and characterization of a secreted, C-terminally processed tyrosinase from the filamentous fungus Trichoderma reesei ppt
... Streptomyces castaneoglobisporus [17]became available, and will enable more detailed analy-sis of the exact reaction mechanisms. The tyrosinasestructure, wherein the active site was located at ... ligandamino acids of the active site are well conservedbetween the proteins, whereas the C-terminal domainsare less conserved.Overexpression of the tyrosinase gene in Trichoderma reeseiTyrosinase ... P. sanguineus tyrosinase.However, at 60 °C, P. sanguineus tyrosinase was alsoinactivated completely within 20 min [40 ]. In general,mammalian and plant-derived tyrosinases are not verythermostable;...
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Báo cáo khoa học: Synthesis and characterization of a new and radiolabeled high-affinity substrate for H+/peptide cotransporters pdf
... current flow reachedsteady state. Currents evoked by PEPT1 or PEPT2 at a given membrane potential were calculated as the difference of the currents measured in the presence and the absence of ... scintillation spectrometry. For each experi-ment, the samples for the protein measurements wereprepared and measured as described previously [20].X. laevis oocytes expressing PEPT1 and PEPT2 and electrophysiologyFemale ... substrate-dependent currents were recorded as the difference measured in the absence and in the presence of substrates.Labeled high-affinity substrate for peptide transporters I. Knu¨tter et al.5908...
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