Báo cáo khoa học: "Production and purification of immunologically active core protein p24 from HIV-1 fused to ricin toxin B subunit in E coli" ppt

... The fact that we were able to < /b> detect the same band by three different antibodies targeting different components of < /b> the chimeric RTB /p24 < /b> protein,< /b> demonstrate the integrity of < /b> the protein < /b> after purification < /b> ... the expression in E. coli of < /b> HIV-1 < /b> p24 < /b> fused < /b> to < /b> the subunit B of < /b> ricin < /b> toxin....

Ngày tải lên: 12/08/2014, 04:21

... shown in E. coli that these enzymes are responsible for the formation of < /b> threon- ine from < /b> glycine in vitro and < /b> in vivo [29]. However, the primary role of < /b> this pathway is believed to < /b> be threonine ... s )1 Æmm )1 ). Discussion Three pathways for threonine degradation are known. Threonine aldolase (EC 4.1.2.5) is responsible for the conversion of < /b>...

Ngày tải lên: 16/03/2014, 14:20

... acids of < /b> the active < /b> site are well conserved between the proteins, whereas the C-terminal domains are less conserved. Overexpression of < /b> the tyrosinase gene in Trichoderma reesei Tyrosinase production ... corresponding cDNA were cloned by PCR and < /b> sequenced in order to < /b> verify the sequence at the genome website, to < /b> exclude PCR mutations and < /b> to...

Ngày tải lên: 19/02/2014, 06:20

... 2005 FEBS 2415 ize to < /b> form pigment. The identity and < /b> mode of < /b> action of < /b> the enzymes involved in the different steps of < /b> mel- anogenesis have long been intensely investigated, in large measure to < /b> elucidate ... Compared to < /b> tyrosinase, disproportionately less effort has been given to < /b> under- standing the role of < /b> peroxidase in the...

Ngày tải lên: 07/03/2014, 17:20

... showed that the deleted NH 2 - terminal b- hairpin of < /b> ribotoxins is involved in the allergenic response to < /b> the wild-type allergens. Binding of < /b> human specific IgG present in the above pool sera to < /b> ... any receptor interaction [1]. Once inside the host cell, ribotoxins inhibit protein < /b> biosynthesis by inactivating the ribosomes leading to < /b> cell death...

Ngày tải lên: 16/03/2014, 19:20

... inform recommendations, this type of < /b> evi- dence was rarely referred in Argentine guidelines [22]. Therefore, networking activities betweeen guideline pro- ducers and < /b> Argentine Cochrane Centers shoud ... Argentine guideline reported conflict of < /b> interests or funding sources. Lack of < /b> Table 4: Scores by item of < /b> the AGREE instrument Domains and < /b> items of...

Ngày tải lên: 11/08/2014, 16:21

... White colo nies cont ained the recombinant bacmid, and < /b> therefore were selected for isolation of < /b> recombinant bacmid DNA. Before DNA was isolated, candidate colonies were streaked to < /b> ensure they were ... baculovirus. lane 1, the protein < /b> expressed in insect cells challenged with negative baculovirus; lanes 2 to < /b> 6, the protein < /b> expressed 1 to < /b> 5 d...

Ngày tải lên: 11/08/2014, 21:21

... revealed unexpected protein< /b> protein < /b> interac- tions interfering with efficient mitochondrial protein < /b> turnover in the case of < /b> human cytosolic aconitase. These results emphasize specific features of < /b> ... transferred to < /b> a poly(vinylidene fluoride) membrane. The band corres- ponding to < /b> m-hIRP1 was cut and < /b> its N-terminal sequence was determined....

Ngày tải lên: 07/03/2014, 10:20

... the contraction seems to < /b> be caused by an increase of < /b> the PEG 200 concentration from < /b> 50% to < /b> 55%, removing water from < /b> the protein.< /b> Comparison with related proteins To < /b> find related proteins in the ... other proteins showed that benzaldehyde lyase belongs to < /b> a group of < /b> closely related ThDP-dependent enzymes. The ThDP cofactors of...

Ngày tải lên: 07/03/2014, 12:20

... recombinant protein < /b> preparation, where the number of < /b> nucleotides was decreased by nuclease treat- ment. The number of < /b> nucleotides per protein < /b> monomer was esti- mated as described in Experimental ... vertebrate lineages, the elucidation of < /b> the function of < /b> the 2-5A synthetase in these invertebrates, particularly in the innate immune system, would...

Ngày tải lên: 23/03/2014, 09:20