Báo cáo khoa học: "Development of real time PCR for detection and quantitation of Dengue Viruses" potx
Báo cáo khoa học: "Development of real time PCR for detection and quantitation of Dengue Viruses" potx
... primer sets for detection of all four serotypes of DENV [11]. In the present study, we describe the development of a DENV- specific TaqMan based real- time PCR for detection and quantitation of all ... 123(5):615-628. BioMed Central Page 1 of 8 (page number not for citation purposes) Virology Journal Open Access Methodology Development of real time PCR fo...
Ngày tải lên: 12/08/2014, 04:21
Báo cáo khoa học: "A multiplex real-time PCR for differential detection and quantification of Salmonella spp., Salmonella enterica serovar Typhimurium and Enteritidis in meats" pps
... al. Detection limit and standard curve of the multiplex real- time PCR The detection limit and standard curve of the multiplex real- time PCR was determined using S. Typhimurium ATCC 14028 and S. Enteritidis ... previous real- time PCR assays [8,27]. These advantages improved the multiplex real- time PCR assay in terms of shortening turnaround time for...
Ngày tải lên: 07/08/2014, 23:22
Báo cáo hóa học: " Research Article Real-Time Cardiac Arrhythmia Detection Using WOLA Filterbank Analysis of EGM Signals" pot
... merged to obtain wideband-subband sig- nals to be used for wideband event detection. The results of the wideband and narrowband detections are then combined for robust detection. As the intention ... of the event detection algorithm. of the subband signals have baseband spectrums, to combine subbands one has to modulate the bands to line them up se- quentially. For merging...
Ngày tải lên: 22/06/2014, 23:20
báo cáo khoa học: " Development-dependent changes in the tight DNA-protein complexes of barley on chromosome and gene level" pot
... 150 ng of the first strand cDNA were used as a template for PCR amplification performed in 30 μl of the reaction mix- ture with a final concentration of 1× PCR buffer (Fermen- tas), 3 mM of MgCl 2 , ... leaves and coleoptiles at two stages of development (Zadoks 07 and 10). In dry seeds most markers of both chromosomes were found in F and R1 fractions. Only four and...
Ngày tải lên: 12/08/2014, 03:20
Tài liệu Báo cáo khoa học: Neuronal growth-inhibitory factor (metallothionein-3): reactivity and structure of metal–thiolate clusters* doc
... is a soft ligand and therefore shows a preference for soft metals. More- over the structures of metallothioneins are not rigid and hence there is little selectivity concerning the size of the ... approach of the two domains. Also, the additional binding of M(II) induced slow, but appreciable, non-covalent dimeriza- tion of MT3 [40% for Zn(II) and 80% for Cd(II)]. Subsequ...
Ngày tải lên: 16/02/2014, 15:20
Tài liệu Báo cáo khoa học: Poly(silicate)-metabolizing silicatein in siliceous spicules and silicasomes of demosponges comprises dual enzymatic activities (silica polymerase and silica esterase) doc
... rational application of silicatein in the fabrication of (new) biomaterials based on layered silica, of titania and of zirconia [32]. This view is based on the finding of a dual role for silicatein ... (Asn) and serine (Ser) [4,12]. In the active center of silicatein, the hydroxyl group of Ser26 and the imidazole of His165 (catalytic diad) have been shown to play key ro...
Ngày tải lên: 18/02/2014, 16:20
Tài liệu Báo cáo khoa học: Structural insights into the substrate specificity and activity of ervatamins, the papain-like cysteine proteases from a tropical plant, Ervatamia coronaria ppt
... understanding of the generic principle of catalysis for this family of proteases. Results and Discussion Fold of ervatamin-A The overall structure and fold of ervatamin-A is simi- lar to that of ... range of substrate concentrations and the time of incubation were standardized for each substrate. An extinction coefficient of 8800 at 410 nm for pNA was used for...
Ngày tải lên: 18/02/2014, 16:20
Tài liệu Báo cáo khoa học: The role in the substrate specificity and catalysis of residues forming the substrate aglycone-binding site of a b-glycosidase docx
... units, in spite of its favorable interac- tion with alkyl moieties. Therefore, the replacement of E194 and K201 may be an important mechanism for changing the specificity of Sfbgly for aglycone. The ... details about the role of different resi- dues of the aglycone-binding site in the stabilization of ES à and the interdependence between the binding of aglycone and the...
Ngày tải lên: 18/02/2014, 17:20
Tài liệu Báo cáo khoa học: Phenol hydroxylase from Acinetobacter radioresistens S13 Isolation and characterization of the regulatory component docx
... curves. Squares and dotted line: data and fitting with PHI/PHO ratio of 0.5. Triangles and continuous line: data and fitting with PHI/PHO ratio of 1. Asterisks and broken line: data and fitting with ... (University of Turin) for mass spectroscopy analysis, to A. Conti and G. Giuffrida (CNR- Torino) for N-terminal sequence determination and to D. Cavazzini (University of...
Ngày tải lên: 21/02/2014, 00:20
Báo cáo khoa học: Protein transport in organelles: The composition, function and regulation of the Tic complex in chloroplast protein import pptx
... channel function (e.g. in ligand-gated and voltage-gated K + channels) [16], and thus could provide an explanation for the observed channel activity of Tic110, as well as for the binding of transit peptides ... of the other isoform. However, analysis of double knockouts of both Hsp93 homologues did not result in the identification of double homozygotes, establishing that Hs...
Ngày tải lên: 07/03/2014, 03:20