Báo cáo khoa học: "Development of a RVFV ELISA that can distinguish infected from vaccinated animals" pps

... pro- vide an easily accessible assay that can be reliably used to distinguish animals that are infected with WT virus from animals that have been vaccinated. This differential ability is important ... AA, Al-Rabeah AM, Turkistani AM, Al-Sayed MO, Abodahish AA, Khan AS, Ksiazek TG, Shobokshi O: Rift Valley fever epidemic in Saudi Arabia: epi- demiological, clinical, and laborato...

Ngày tải lên: 12/08/2014, 04:21

... pro- vide an easily accessible assay that can be reliably used to distinguish animals that are infected with WT virus from animals that have been vaccinated. This differential ability is important ... Journal Open Access Research Development of a RVFV ELISA that can distinguish infected from vaccinated animals Anita K McElroy 1,2 , César G Albariño 1 and St...

Ngày tải lên: 12/08/2014, 04:22

... at RT. After adding ABTS (KPL, USA) and 30 min’s incubation at RT, absorbance was measured at 405 nm. Sandwich ELISA The sandwich ELISA was performed on microtiter plates. Each well was coated ... purifi ed f lagellin. About 10 µg of proteins were loaded in each lane. Table 1. Specificity analysis of antibodies (Abs) by ELISA with bacterial species Bacterial strains Monoclonal Abs...

Ngày tải lên: 07/08/2014, 18:21

... 5¢-CTC GAGATGGATAAAGTTTTAAACAGAG-3¢ and LTA- 1R, 5¢-TGAAGGCAAATCTCTGGAC-3¢ for the former, and LTA–M2F, 5¢-CAGCTGTTTTGCTTGAATTATG-3¢ and LTA–2R, 5¢-GAATTCATTATGTTTCAGGTTCA GGGG-3¢ for the latter. The ... organ-specific blood vascular and lymphatic endothelial cells of the mouse Takashi Yamaguchi, Taeko Ichise, Osamu Iwata, Akiko Hori, Tomomi Adachi, Masaru Nakamura, Nobuaki Yoshida and Hirot...

Ngày tải lên: 18/02/2014, 17:20

... University of Chicago, Department of Lin- guistics. variety of applications are considered in evaluating the theoretical and practical attributes of several previous algorithms. As a major part of ... sonal communication]) or mathematical analysis of a body of language, often require matched stems. (So does stemming as part of an information-retrieval sys- tem, th...

Ngày tải lên: 16/03/2014, 19:20

... (5¢-CCTGTCAGATCTCCGCCAT GGCTAACAATGCATCTCT-3¢), and a BamHI site (bold) was introduced into the reverse primer (5¢-TCTCC CGGATCCAAAGAGAAATACCCATA-TA-3¢) to facili- tate vector–insert ligation. Amplification ... showed that FRET was enhanced by the addition of a GnRH agonist but not by an antagonist (Fig. 7), suggesting that the GnRH agonist facilitates receptor association. Although...

Ngày tải lên: 30/03/2014, 20:20

... mammals [8]. Because of evolutionary difference, chicken IgY reacts with more epitopes on a mammalian antigen, producing an amplification of the signal. IgY also has the advantage in that it avoids ... sanitary state of farms, pig sera were randomly collected and concentration of IL-6 in the sera was measured with the antigen capture -ELISA. The capture -ELISA with the optimal co...

Ngày tải lên: 07/08/2014, 18:20

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Ngày tải lên: 07/08/2014, 18:21

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Ngày tải lên: 07/08/2014, 20:23

... if the same stands are surveyed. These kind of disadvantages caused by using categorical variables can be avoided by the use of fea- tures on interval scales. These interval scale variables must ... district Fuhrberg near Hannover [28]. This region in north-western Germany is characterised by a subatlantic climate with an annual precipitation of 700 mm and a mean annual temperatu...

Ngày tải lên: 08/08/2014, 14:22