Báo cáo khoa học: " A novel virus that infecting hypovirulent strain XG36-1 of plant fungal pathogen Sclerotinia sclerotiorum" pptx

... Journal Open Access Research A novel virus that infecting hypovirulent strain XG36-1 of plant fungal pathogen Sclerotinia sclerotiorum Liyan Zhang 1,2 , Yanping Fu 2 , Jiatao Xie 2 , Daohong Jiang* 1,2 , ... be a novel mycovirus associated with hypovirulence of plant fungal pathogen. Methods Fungal strains, media and culture S. sclerotiorum strain XG...

Ngày tải lên: 12/08/2014, 04:21

... to electrophoretic analysis on 1% agrose gel. Data analysis Each test had three to five replicates, and data from the experiments were analyzed using an analysis of variance (ANOVA) in SAS (SAS Software, NJ). ... fungus Cryphonectria parasitica that is infectious as particles and related to the Coltivirus genus of animal pathogens. Journal of Virology 2004, 78:892-898. 13. Sasaki A,...

Ngày tải lên: 12/08/2014, 04:22

... oxidation of methionine and variable deamidation of asparagine and glutamine. Parent and fragment mass toler- ances were set to 1 Da. Up to two missed cleavages and half tryptic peptides were allowed. ... potentially exhibits amine oxidase activity [26]. Hence, we may speculate that hmeprin has activity similar to BMP-1 ⁄ TLD-like metalloendopeptidases in that it acts as a procollag...

Ngày tải lên: 07/03/2014, 06:20

... that the coding region begins at this ATG. A polyadenylation signal, AATAAA, exists 16 bases upstream from the start of the poly (A) + tail. Fig. 2. HPLC analysis of the intrinsic ligand of Papi ... from dark-adapted or light-adapted retinas, and analyzed by HPLC. The molar ratio of all-trans,11-cis and 13-cis 3-hydroxyretinol was then calculated based on the absorbance and the m...

Ngày tải lên: 17/03/2014, 03:20

... ethanol, sonicated and extracted overnight at +4 °C [23]. After centri- fugation, the pellet was discarded and a sample of the supernatant was withdrawn for radioimmunoassay of insulin and glucagon ... Both end-plate potentials of the ion trap were set at 1.5 V and the duration of the electron pulse was 100 ms. Data acquisition and handling Primary data analysis was performed on a...

Ngày tải lên: 17/03/2014, 10:20

... with Flag–STA- T 5A_ FL and STAT 5A_ DE18–EGFP (B–D) or Flag–STAT 5A_ FL alone (E–G). Flag–STAT 5A_ FL was detected with a primary antibody against STAT 5A_ FL and Alexa Fluor 546-conjugated secondary antibody ... domain, a DNA-binding domain, an SH2 domain, and a C-terminal transactivation domain. The deletion of exon 18 (52 bp) in the STAT 5A_ DE18 mRNA results in a translational...

Ngày tải lên: 23/03/2014, 04:20

... A novel G-quadruplex motif modulates promoter activity of human thymidine kinase 1 Richa Basundra 1, *, Akinchan Kumar 1, *, Samir Amrane 2, *, Anjali Verma 1 , Anh Tua ˆ n Phan 2 and Shantanu ... reported that parallel-stranded G-quadruplex- es give a positive peak at 260 nm and a negative peak at 240 nm, whereas antiparallel-stranded G-quadru- plexes give a positive peak at 29...

Ngày tải lên: 29/03/2014, 21:20

... FEBS A novel phosphorylated glycoprotein in the shell matrix of the oyster Crassostrea nippona Tetsuro Samata, Daisuke Ikeda, Aya Kajikawa, Hideyoshi Sato, Chihiro Nogawa, Daishi Yamada, Ryo Yamazaki ... Yamazaki and Takahiro Akiyama Laboratory of Cell Biology, Faculty of Environmental Health, Azabu University, Sagamihara, Japan Subsequent to the pioneering work of Miyamoto et al....

Ngày tải lên: 30/03/2014, 04:20

... (Brassica napus var. napobrassica) [6,7]. P. brassicae is mostly associated with post-harvest damage that limits the marketability of cab- bage heads and can reach up to 90% losses [8-10]. Although ... inoculation experiments. The system has the advantage that zoospores are produced continuously over a period of at least one month. Background Plants can be affected by a broad rang...

Ngày tải lên: 12/08/2014, 03:21

... and anti- sense strand (5¢-ATGAGTTTTCCAGAGCAACTT-3¢), and siRNA was synthesized using DNA templates (sense strand, 5¢-AAATGAGTTTTCCAGAGCAACCCTGTCTC-3¢; anti- sense strand, 5¢-AAGTTGCTCTGGAAAACTCATCCTG TCTC-3¢) ... long dsRNAs into effector siRNAs. Human Dicer contains a single PAZ domain, a single dsRNA-binding domain, and ATPase ⁄ helicase domains, as well as two RNase III- like domains...

Ngày tải lên: 16/03/2014, 02:20