Báo cáo khoa học: " A novel virus that infecting hypovirulent strain XG36-1 of plant fungal pathogen Sclerotinia sclerotiorum" pptx

... JournalOpen AccessResearch A novel virus that infecting hypovirulent strain XG36-1 of plant fungal pathogen Sclerotinia sclerotiorumLiyan Zhang1,2, Yanping Fu2, Jiatao Xie2, Daohong Jiang*1,2, ... be a novel mycovirus associated withhypovirulence of plant fungal pathogen. Methods Fungal strains, media and cultureS. sclerotiorum strain XG36-1 was isolated from a typicallesion on stem of ... mature colony, with theaverage number of sclerotia at 5 sclerotia/plate, while strain XG-13 had 12 sclerotia/plate. Strain XG36-1 wasalmost incapable of inducing any lesions on detachedleaves...

Báo cáo khoa học: "A novel virus that infecting hypovirulent strain XG36-1 of plant fungal pathogen Sclerotinia sclerotiorum" potx

... toelectrophoretic analysis on 1% agrose gel.Data analysisEach test had three to five replicates, and data from theexperiments were analyzed using an analysis of variance(ANOVA) in SAS (SAS Software, NJ). ... fungusCryphonectria parasitica that is infectious as particles andrelated to the Coltivirus genus of animal pathogens. Journal of Virology 2004, 78:892-898.13. Sasaki A, Kanematsu S, Onoue M, Oikawa Y, Nakamura ... be a novel mycovirus associated withhypovirulence of plant fungal pathogen. Methods Fungal strains, media and cultureS. sclerotiorum strain XG36-1 was isolated from a typicallesion on stem of...

Báo cáo khoa học: A novel 2D-based approach to the discovery of candidate substrates for the metalloendopeptidase meprin pot

... oxidation of methionine and variable deamidation of asparagine and glutamine. Parent and fragment mass toler-ances were set to 1 Da. Up to two missed cleavages andhalf tryptic peptides were allowed. ... potentiallyexhibits amine oxidase activity [26]. Hence, we mayspeculate that hmeprin has activity similar to BMP-1 ⁄TLD-like metalloendopeptidases in that it acts as a procollagen C protease as well as an activator ... design of a simple image analysis procedure that unmasked step-by-step qualitative differences amonggel replicates. The modular character of this type of analysis allowed the integration of all...

Báo cáo khoa học: A novel retinol-binding protein in the retina of the swallowtail butterﬂy, Papilio xuthus docx

... that thecoding region begins at this ATG. A polyadenylation signal,AATAAA, exists 16 bases upstream from the start of thepoly (A) +tail.Fig. 2. HPLC analysis of the intrinsic ligand of Papi ... from dark-adapted or light-adapted retinas, and analyzed byHPLC. The molar ratio of all-trans,11-cis and 13-cis 3-hydroxyretinolwas then calculated based on the absorbance and the molar extinctioncoeﬃcient ... Science, Yokohama City University, Yokohama, Kanagawa;2Graduate School of Frontier Biosciences,Osaka University, Toyonaka, Osaka, JapanRetinoid-binding proteins are indispensable for visual cyclesin...

Báo cáo khoa học: A novel mass spectrometric approach to the analysis of hormonal peptides in extracts of mouse pancreatic islets ppt

... ethanol, sonicatedand extracted overnight at +4 °C [23]. After centri-fugation, the pellet was discarded and a sample of thesupernatant was withdrawn for radioimmunoassay of insulin and glucagon ... Both end-plate potentials of the iontrap were set at 1.5 V and the duration of the electronpulse was 100 ms.Data acquisition and handlingPrimary data analysis was performed on a workstationrunning ... theXMASSTMsoftware (Bruker Daltonics). Inthe direct infusion experiment, a spectrum of 200 scans,512 K data points was collected. The spectrum wascalibrated using a dataset of a sample of standard...

Báo cáo khoa học: A novel splicing variant form suppresses the activity of full-length signal transducer and activator of transcription 5A pot

... with Flag–STA-T 5A_ FL and STAT 5A_ DE18–EGFP (B–D) or Flag–STAT 5A_ FL alone (E–G). Flag–STAT 5A_ FL was detected with a primary antibody againstSTAT 5A_ FL and Alexa Fluor 546-conjugated secondary antibody ... domain, a DNA-binding domain, an SH2 domain, and a C-terminal transactivation domain. The deletion of exon 18 (52 bp)in the STAT 5A_ DE18 mRNA results in a translational frameshift from Ala688. ... transcriptional regu-lation, we isolated a novel splicing variant of STAT 5A, STAT 5A_ DE18, from a cDNA library of the mousebrainstem. The variant protein was generated by a frameshift in the C-terminal...

Báo cáo khoa học: A novel G-quadruplex motif modulates promoter activity of human thymidine kinase 1 docx

... A novel G-quadruplex motif modulates promoter activity of human thymidine kinase 1Richa Basundra1,*, Akinchan Kumar1,*, Samir Amrane2,*, Anjali Verma1, Anh Tuaˆn Phan2andShantanu ... reported that parallel-stranded G-quadruplex-es give a positive peak at 260 nm and a negative peakat 240 nm, whereas antiparallel-stranded G-quadru-plexes give a positive peak at 290–295 nm and a ... intramolecularAAATCTCCCGCCAGGTCAGCGGCCGGGCGCTGATTGGCCCCATGGCGGCGGGGCCGGCTCGTGATTGGCCAGCACGCCGTGGTTTAAAGCGGTCGGCGCGGGAACCAGGGGCTTACTGCGGGACGGCCTTGGAGAGTACTCGGGTTCGTGAACTTCCCGGAGGCGCAATGAGCT A BLoop35′3′Loop2Loop1GGGGGGGGFig. 1. G-quadruplex motif and...

Báo cáo khoa học: A novel phosphorylated glycoprotein in the shell matrix of the oyster Crassostrea nippona pptx

... FEBS A novel phosphorylated glycoprotein in the shell matrix of the oyster Crassostrea nipponaTetsuro Samata, Daisuke Ikeda, Aya Kajikawa, Hideyoshi Sato, Chihiro Nogawa, Daishi Yamada,Ryo Yamazaki ... Yamazaki and Takahiro AkiyamaLaboratory of Cell Biology, Faculty of Environmental Health, Azabu University, Sagamihara, JapanSubsequent to the pioneering work of Miyamoto et al.[1], Sudo et al. ... the OM of C. nippona. The same amount of sample was applied to each lane.Lane M, molecular mass standards; lane A, CBB staining; lane B,Stains-all staining; lane C, negative staining; lane D,...

báo cáo khoa học: " A novel method for efficient and abundant production of Phytophthora brassicae zoospores on Brussels sprout leaf discs" pot

... (Brassica napus var.napobrassica) [6,7]. P. brassicae is mostly associated withpost-harvest damage that limits the marketability of cab-bage heads and can reach up to 90% losses [8-10].Although ... inoculation experiments. The system has the advantage that zoospores are produced continuously over a period of at least one month.BackgroundPlants can be affected by a broad range of plant- patho-genic ... oomycete -plant interactions, allowing concur-rent molecular analysis of the host as well as the patho-gen. A disadvantage of P. brassicae is the fact that generatingzoospores is troublesome. In nature,...

Báo cáo khoa học: A new and efﬁcient method for inhibition of RNA viruses by DNA interference pptx

... and anti-sense strand (5¢-ATGAGTTTTCCAGAGCAACTT-3¢), andsiRNA was synthesized using DNA templates (sense strand,5¢-AAATGAGTTTTCCAGAGCAACCCTGTCTC-3¢; anti-sense strand, 5¢-AAGTTGCTCTGGAAAACTCATCCTGTCTC-3¢) ... long dsRNAsinto effector siRNAs. Human Dicer contains a singlePAZ domain, a single dsRNA-binding domain, andATPase ⁄ helicase domains, as well as two RNase III-like domains and a domain of unknown ... several assays: (a) plantsinoculated with only TMV; (b) plants inoculated withTMV and long dsDNAs; (c) plants inoculated withTMV and siRNA; (d) plants inoculated with shortantisense ssDNA (as-DNA);...

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