Báo cáo khoa học:" Transduction of rat pancreatic islets with pseudotyped adeno-associated virus vectors" doc
... numbers of rat islets, versus murine islets, also makes the use of rat islets desirable. Therefore, we examined the transduction of rat islets by pseudotyped AAV vectors. Results Transduction of Rat ... [9]. The amount of insulin contributed Transduction of rat islets with a high MOI of pseudotyped AAV2/8 vectors and a high Ad5 MOIFigure 2 Transdu...
Ngày tải lên: 12/08/2014, 04:21
... phagosomes without perturbation of the formation of intracellular organelles and without alteration of the morphology of vacuolar compartments, resulting in the profound inhibition of the endosomal ... without any detectable formation of its mature form (Fig. 5C). In addition, we found that the level of expression of this mutant A B C Fig. 4. Pulse–chase analysis of the acti...
Ngày tải lên: 16/03/2014, 14:20
... Protein Preparation command of glide. Interactions of probe atoms with proteins were calculated with the Receptor Grid Generation command of V. Bauerova ´ -Hlinkova ´ et al. Structures of RNase Sa2–mononucleotide ... [33], with yields of 10–50 mg from 1 L of cul- ture medium. The crystallization of RNase Sa2 with a free active site was performed as described previously [...
Ngày tải lên: 18/02/2014, 11:20
Tài liệu Báo cáo khoa học: Inhibition of pea ferredoxin–NADP(H) reductase by Zn-ferrocyanide docx
... presence of Fd or F ld was not affected by the presence of up to 1 m M ZnSO 4 . The effect of equimolar concentrations of Zn 2+ and ferrocyanide on diaphorase activities with different sub- strates ... of th e F NR for the different s ubstrates were examined at pH 7.5. Plots for the inhibitions of FNR diaphorase a ctivities with i ncreasing concentration o f Zn-ferrocyanide (r...
Ngày tải lên: 19/02/2014, 16:20
Tài liệu Báo cáo khoa học: "Demonstration of the UAM CorpusTool for text and image annotation" docx
... problems. Often, the annotation scheme has been built into the software, or the software has been limited in that they allow only certain types of annotation to take place. A small number of systems ... cross- layer searches of the corpus. 1 Introduction In the last 20 years, a number of tools have been developed to facilitate the human annotation of text. These have been nec...
Ngày tải lên: 20/02/2014, 09:20
Tài liệu Báo cáo khoa học: Changes in rat liver mitochondria with aging Lon protease-like activity and N e-carboxymethyllysine accumulation in the matrix doc
... of disrupted organelles in preparations from both old and adult rats, as well as the same level of respiratory chain activity in the two groups of animals. Recently, age-related alterations of ... Oxygen-consumption rates are expressed as ng atoms of oxygen consumed per minute and per mg protein. The rate of oxygen consumption in state 3 and in state 4, respiratory control ratio (...
Ngày tải lên: 20/02/2014, 11:20
Báo cáo khoa học: Reduction of a biochemical model with preservation of its basic dynamic properties doc
... the 20D model, and the 20L8D model has a period of 7.2 s with the literal inter- pretation of concentrations and a period of 22 s with the concentrations pooled as indicated. Consequently, we performed ... optimization at the operating point with pooled concentrations (see Tables 6–8 of Hynne et al.) [30]. Construction of the 20E6D model by ENVA Model reduction by means of l...
Ngày tải lên: 07/03/2014, 11:20
Báo cáo khoa học: Interaction of synthetic peptides corresponding to hepatitis G virus (HGV/GBV-C) E2 structural protein with phospholipid vesicles doc
... peptides. Leakage of 70% was reached for E2(7–26) at a peptide to lipid ratio of 2 : 1. For W-E2(279–298), complete lysis of the LUVs was reached at a peptide to lipid ratio of 1 : 1. For the E2(7–26) ... titration of the peptides with Ptd- Cho ⁄ PtdSer vesicles was carried out in the absence of salt. The E2(7–26) peptide showed a significantly higher blue shift of Trp fluoresce...
Ngày tải lên: 07/03/2014, 17:20
Báo cáo khoa học: Reactions of gold(III) complexes with serum albumin docx
... temperature with k ex ¼ 295 nm; BSA 5 · 10 )5 M was titrated with [Au(bi- py c -H)(OH)][PF 6 ] at the ratios [Au(bipy c -H)(OH)][PF 6 ]/ BSA r ¼ 0.5–5.0 (where r is moles of drug per mole of BSA). Ultrafiltration ... measurements The interaction of all complexes with BSA was analysed by monitoring the electronic spectra of freshly prepared solutions of each complex after mixing...
Ngày tải lên: 07/03/2014, 21:20
Báo cáo khoa học: Prevalence of intrinsic disorder in the hepatitis C virus ARFP/Core+1/S protein doc
... pre-equilibrated with buffer A supplemented with 10 m m imidazole and one tenth of the antiprotease concentration recommended by the manufacturer. The column was washed with buffer A sup- plemented with ... showed a high number of overlapping peaks, impeding the accurate counting of peaks. In contrast, the HSQC spectrum of HCV-1b Core+1/S allows the counting of a number of...
Ngày tải lên: 15/03/2014, 09:20