Báo cáo khoa học: "SARS coronavirus protein 7a interacts with human Ap4A-hydrolase" ppsx

... coronavirus protein 7a interacts with human Ap 4 A-hydrolase Natalia Vasilenko, Igor Moshynskyy, Alexander Zakhartchouk * Abstract The SARS coronavirus (SARS-CoV) open reading frame 7a (ORF 7a) ... accessory protein. It has no significant sequence homology with any other known proteins. The 7a protein is present in the virus parti- cle and has been shown to interact w...

Ngày tải lên: 12/08/2014, 04:21

... protein C (461 residues) shares 69% sequence identity with its human ortholog. Interspecies experiments suggest that there is an incompatibility between mouse and human protein C, such that human protein ... the mouse protein C sequence. Highlighted residues are different with respect to wild-type mouse protein C. Sequences used for comparison were obtained from NCBI with...

Ngày tải lên: 18/02/2014, 06:20

... C-terminus may facilitate interactions with other intracellular proteins rather than executing a catalytic reaction. mSULT2B1b interacts with cytoskeletal proteins through its proline ⁄ serine-rich ... (Tokyo, Japan). GST pulldown assay and protein identification by PMF analysis To assess protein protein interactions between mSULT2B1b and intracellular proteins, stably transfected ce...

Ngày tải lên: 06/03/2014, 22:21

... within the short stretch of DNA in the TTR gene, as well as the availability of highly enriched HNFs in liver cells, make it likely that protein protein interac- tions between different HNF proteins ... individually mutated HNF binding sites was incubated with nuclear protein extracted from HepG2 cells; the DNA protein complexes were analyzed by ELISA with antibodies against HNF-4a,...

Ngày tải lên: 23/03/2014, 03:20

... patients with human immunodeficiency virus is debated because the spleen is a replication site, especially during the asymptomatic phase of this infection. We present a case of a patient with human ... Dumaurier MJ, Gratton S, Wain-Hobson S, Cheynier R: The majority of human immunodeficiency virus type 1 particles present within splenic germinal centres are produced locally. J Gen V...

Ngày tải lên: 10/08/2014, 22:24

... for this process. In addition, whether Stau1 interacts first with NC or with the genomic RNA to recruit the other partner or independently interacts with each of them through dif- ferent pathways ... NC-p1-YFP proteins. These proteins were then tested for their capacity to inter- act with Stau1 55 using the BRET assay in live 293T cells (Figure 2A). This technique allows us to de...

Ngày tải lên: 13/08/2014, 05:20

... summarize the properties of the three protein classes, with the main focus being on RNA chaperones and annealer proteins. Specifically binding proteins A specific protein cofactor binds to its RNA ... [60]. (d) For the human mRNA-binding protein hnRNP A1 [61], the Xenopus laevis protein X1rbpa [54], the trypanosome guideRNA-binding protein RBP16 [62], and the Escherichia coli pro...

Ngày tải lên: 14/02/2014, 19:20

... mixed with GST alone as a control or with GST-tagged proteins. (B) The HIS-PTI1-4 protein was mixed with GST alone as a control or with GST-tagged proteins. In (A) and (B), the GST-tagged proteins ... together HIS-OXI1 and GST-PTI1-4 proteins or HIS-PTI1-4 and GST-OXI1 proteins, the GST-tagged proteins were pulled down with glutathi- one-agarose beads. The proteins were then detec...

Ngày tải lên: 14/02/2014, 19:20

... of the protein was estimated on SDS ⁄ PAGE. The protein concentration was measured using Bradford reagent [27]. A similar protocol was used for the expression and purification of HPRT, with the ... potential antiparasital drug target [7]. A homolog with 65% identity to HPRT, phos- phoribosyltransferase domain containing protein 1 (PRTFDC1) is present in the human genome. A recent s...

Ngày tải lên: 15/02/2014, 01:20

... encapsidated along with several viral and cellular proteins. Assembly-competent Gag molecules are bound to the plasma membrane and may directly interact with molecules of the viral envelope polyprotein, ... cell. Keywords capsid; conformational stability and dynamics; human immunodeficiency virus; molecular recognition; protein association; protein conformation; protein structure–...

Ngày tải lên: 18/02/2014, 06:20