Báo cáo khoa học: " Inactivation of respiratory syncytial virus by zinc finger reactive compounds" ppsx

... al.: Inactivation of respiratory syncytial virus by zinc finger reactive compounds. Virology Journal 2010 7:20. Publish with BioMed Central and every scientist can read your work free of charge "BioMed ... identified as reactive towards the zinc fin- ger motif in the NC proteins might also react with the zinc finger motif of the M2-1 protein of RSV. There...

Ngày tải lên: 12/08/2014, 04:21

... the environment of reactive sites of annexin II by GSNO. We tested whether such changes influence the GSNO inhibition of the activity of annexin II. AIIt (5 lg)waspreincubatedin50lLof buffer containing ... the binding of annexin II, the reactive sites were hidden by membrane; (b) the binding of AIIt to membrane causes a conformational change [65], such changes may bury the...

Ngày tải lên: 08/03/2014, 10:20

... l-DOPA productivity of T15A was at least two-fold higher than that with C. freundii Fig. 5. Inactivation of TPL enzymes by L-DOPA and its reactivation by PLP. Timecourse profiles of inactivation and ... eventually led to the depletion of the cofactor PLP, accompanied by the inactivation of the enzyme. HPLC and 1 H NMR analyses of the purified adduct revealed that the in...

Ngày tải lên: 16/03/2014, 12:20

... bovine serum amine oxidase, two different mechanisms of substrate-induced inactivation have been proposed. One consists of a slow oxidation by H 2 O 2 of a conserved residue in the reduced enzyme after ... formation of the 350 nm band, was induced by some aldehydes, notably putrescine. Some mechanisms of inactivation are proposed. Keywords: copper amine oxidase; trihydroxyphe...

Ngày tải lên: 19/02/2014, 12:20

... 2003 Stimulation of glycogen synthesis but not activation of glycogen synthase by insulin can be explained by inactivation of phosphorylase To determine the role of inactivation of phosphorylase ... activation of hepatic glycogen synthase by insulin involving either inactivation of GSK-3 or inactivation of phosphorylase. Insulin phosphorylates and inactivates (–)...

Ngày tải lên: 31/03/2014, 01:20

... solu- tion of ThT and the fluorescence intensity of the solu- tion was measured at 482 nm. Figure 4A shows the kinetics of aggregation of a-synuclein in the presence of 100 lm MPTP and the effect of 50 ... shown to inhibit fibrillation of a-synuclein by promoting the formation of nonamyloidogenic oligomers. Fibrillation of a-synuclein is accelerated in the presence of pest...

Ngày tải lên: 14/02/2014, 19:20

... weights of 161.8, 28.2 and 13.2 kDa, respectively. The formation of the cathepsin B ⁄ cystatin C complex was seen as a shift of the elution peak of cathepsin B (Fig. 6A). The addition of 2A2 mAb ... microliters of Abz-GIVRAK(Dnp)-OH (final concentration 1 lm) and 10 lL of 2A2 mAb or NaCl ⁄ P i were added to a well of a black microtiter plate and the reaction was initiated by...

Ngày tải lên: 18/02/2014, 11:20

... hydrolysis of triolein by purified recombinant rat adipocyte HSL, with a decrease in the effect of bis-ANS upon PKA phosphorylation of HSL. The interaction of HSL with bis-ANS was found to have a K d of ... typically incubated for a period of 30 min at 37 °C before the reaction was quenched by the addition of 3.25 mL of methanol ⁄ chloroform ⁄ heptane (10 : 9 : 7) and 1.1 m...

Ngày tải lên: 18/02/2014, 11:20

... formation of O 2 – through the partial reduc- tion of oxygen by one electron. Subsequent reduction by one or two electrons can yield H 2 O 2 , and HO, respect- ively [46]. However, the main source of ... else- where [11,12]. The lack of mitochondrial contamina- tion with cytosolic NOS was assessed by the absence of any detectable NOS activity and nitrite levels in the supernat...

Ngày tải lên: 19/02/2014, 00:20

... phos- phorylation. Moreover, expression of silent mutants of Shc isoforms showed that only p46 Shc and p52 Shc res- cued the effect of the siRNA. Overexpression of p66 Shc not only failed to rescue ... suggested the presence of a signal transduction pathway induced either directly by sE-cad or indirectly by the disruption of cell–cell con- tact [55]. Here we show that disrupti...

Ngày tải lên: 19/02/2014, 05:20