Báo cáo khoa học: "Molecular characterization of the virulent infectious hematopoietic necrosis virus (IHNV) strain 220-90" doc

... in other IHNV strains. The first 15 of the 16 nucleotides at the 3’- and 5’-termini of the genome are complementary, and the first 4 nucleotides at 3’-ends of the IHNV are identical to other ... Vakharia 1* Abstract Background: Infectious hematopoietic necrosis virus (IHNV) is the type species of the genus Novirhabdovirus, within the family Rhabdoviridae,...

Ngày tải lên: 12/08/2014, 04:21

... members of the Novirhabdovirus genus. The termini of the viral genome have conserved sequences at the 3'-end (CAUAG/UU) and 5'-end (G/AAUAUG) as other members of the Novirhabdovirus genus. ... Novirhabdovirus genus. The first 4 nt of the leader sequence VHSV are complementary to the last 4 nt sequence of the trailer region (Fig 2B). The length of the...

Ngày tải lên: 12/08/2014, 04:20

... pathways. Neither endocytic (Fig. 6) nor biosynthetic transport to the vacuole (Fig. 7) is affected in the absence of Osh6p or the presence of a high level of Osh6p. The discrepancy may be due to the ... charac- terization of Osh6p, one of the seven OSBP homo- logs in yeast. We show, for the first time, a direct binding of PA by the ORD domain and the cellular loc...

Ngày tải lên: 07/03/2014, 21:20

... a molecular mass of 53 kDa and a pI of 7.5 (Fig. 2A), corresponding to the predicted size of the putative pro- tein calculated from the HlSCP1 amino acid sequence. To detect the localization of endogenous ... occurs in the food vacuole, where plasmepsin, a member of the aspartic protease family, initially cleaves in the conserved hinge region of the Hb alpha chains...

Ngày tải lên: 16/03/2014, 10:20

... Scheme of the three-hybrid strategy used in the screen. (B) Number of colonies identified in each step of the screen. (C) Distribution of the 63 distinct sequences in relation of their position in the ... influ- enced either by nucleotides outside of the consensus motif or by small variations within the consensus. The interaction of APUM-2 with the FAS-2 tran- sc...

Ngày tải lên: 18/02/2014, 06:20

... between the two steps of the TES reaction The substrate guanosine to be excised (G 1 ) and its 2¢-OH group are required for the second step of TES [48], similar to (if not the same as) the role of the ... that there is room for improve- ment in terms of the rate of reaction. This would be beneficial not so much in terms of the rate of the over- all reaction, a...

Ngày tải lên: 18/02/2014, 18:20

... ligase and the role of Pin1. Pin1 is brought in with the cyclin E substrate, through interaction with the pSer384-Pro motif. When the com- plex contains the CDC4a isoform, the iso- merase activity of ... for destabilizing the double bond character of the pThr-Pro bond [26]. Further evidence is available for the catalytic mecha- nism of Pin1 resembling more closely th...

Ngày tải lên: 07/03/2014, 05:20

... explained by the observation that the amount of H 2 O 2 measured, was influenced by the time period between the NADH conversion and the actual measurement of H 2 O 2 . Apparently, the amount of H 2 O 2 in ... on the absolute activity, DTT also raised the stability about twofold (half-life 83 min) (Fig. 7). The product of the NADH oxidase reaction NADH oxidases can p...

Ngày tải lên: 08/03/2014, 02:21

... 2002 The understanding of the fine tuning of MGP gene expression requires further investigation and the use of different vertebrate systems may be useful in bringing new insights into the mat ter of ... identified 5¢ end of the xMGP cDNA [4]. The lower group of bands, identified as site ÔBÕ in Fig. 4, probably corresponds to a premature arrest of the reverse transcrip...

Ngày tải lên: 08/03/2014, 10:20

... (S) or the immunoprecipitates (IP) was measured, and is expressed as the percentage of the total activity in the 10 lL of concentrated Sup4 (Input). The results shown are the aver- ages of three ... localization of the expressed protein was moni- tored. The results, as shown in Fig. 8B, clearly demonstrate the ability of N57 to promote the nuclear accumulation o...

Ngày tải lên: 08/03/2014, 10:20