Báo cáo khoa học: "The ORF59 DNA polymerase processivity factor homologs of Old World primate RV2 rhadinoviruses are highly conserved nuclear antigens expressed in differentiated epithelium in infected macaques" pps
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The ORF59 DNA polymerase processivity factor homologs of Old
World primate RV2 rhadinoviruses are highly conserved nuclear
antigens expressed in differentiated epithelium in infected
macaques
A ... recog-
Comparison of the ORF59 homologs of Old World primate RV1 and RV2 rhadinovirusesFigure 1 (see previous page)
Co...
... Sox9-binding sites of the
AP-2e promoter (Sox9_1 and Sox9_2). Incubation of
in vitro-synthesized Sox9 with the labeled oligonucleo-
tides containing the Sox9-binding sites resulted in a
strong DNA protein ... all share a conserved basic-helix–
span–helix DNA- binding and dimerization domain
at their C-terminus, and a less conserved proline and
glutamine-rich transactivation doma...
... Gaudin C &
Be
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laı
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ch JP (1999) Sequence analysis of scaffolding
protein CipC and ORFXp, a new cohesin-containing
protein in Clostridium cellulolyticum: comparison of
various cohesin domains ... In general, cellulosomes are composed of a
scaffolding protein devoid of enzymatic activity which
binds the complexes to the substrate via its carbo-
hydrate-binding module (CBM)....
... using myoglobin.
An envelope of multiply charged signals was obtained and
deconvoluted using maxent1 software to give the molecular
mass of the protein.
Mapping the lipoylation of E2
Recombinant ... How-
ever, in addition to the identity of the neighbouring
residues, it is the exact positioning of the lysine in the
lipoyl domain that is fundamental to target lysine recog-
nition...
... binding.
Determination of disulfide linkages in the CFC domain
Determination of whether there are free thiol groups in the
protein was done by alkylation of the protein with NEM
under nonreducing ... 1–4, 8 in domain 5
NELL1 (rat) 6 5 10 in domains 1–4, 8 in domain 5
NELL2 (human and rat) 6 5 10 in domains 1–4, 8 in domain 5
Protein kinase C (BP) 6 5 10 in domains 1–4,...
... assigning a single point for each of the fol-
lowing: (1) serum ferritin ≥ 1,000 ng/mL, (2) greater than
25 transfused units of red cells, and (3) marrow iron stain
of 6+. The sum of points, ranging ... overload in the transplant setting influences
mortality by an alternate mechanism of action, differing
from the classic model of chronic free-radical induced
organ damage. Intere...
... role in binding the incoming tem-
plate in both PolC and DnaE. The ability to bind sin-
gle-stranded DNA has indeed been demonstrated for
the E. coli DnaE OB domain [12,16]. The very N-ter-
minal ... DnaE C-terminal domain
are attached to the OB domain, which likely binds the
DNA template in both polymerases. Although the
exact positions of the corresponding OB domains in
PolC...
... C-terminus of Y-family DNA polymerases
also plays a role in modulating their enzymatic activity
through protein–protein interactions. Elucidation of
the precise role of the C-terminal domain in ... C-termini that is essential for their targeting to
the replication machinery in vivo [3]. This region also
contains novel ubiquitin-binding domains that are evo-
lutionarily conser...
... Thus, the stereochemistry of
the B[a]P-N
2
-dG adducts in DNA does not in uence
DNA binding in the case of M.SssI, but, in contrast,
does affect DNA binding in the case of M.HhaI.
The (+)-trans-B[a]P-N
2
-dG ... residue
positioned in the DNA minor groove severely inhibits
DNA binding to M.HhaI by perturbing the minor-
groove DNA M.HhaI contacts and does not signifi-...