... Respir Cell Mol Biol 13, 547–554.9 Giovine M, Pozzolini M, Fenoglio I, Scarfı`S, GhiazzaM, Benatti U & Fubini B (2003) Crystalline silica incu-bated in ascorbic acid acquires a higher ... process named silicosis.Alveolar macrophages initiate the disease through their activation, which isthe origin of the later dysfunctions. Ascorbic acid is known to selectivelydissolve the quartz ... synthesized from 1 lg RNA in theappropriate buffer containing 5 mm MgCl2, 40 U ribonuc-lease inhibitor (RNASEOUT; Invitrogen), 10 mm dithiothrei-tol, and 200 U SuperscriptTMIII (Invitrogen),...