Báo cáo khoa học: "Bovine viral diarrhea virus NS4B protein is an integral membrane protein associated with Golgi markers and rearranged host membranes" ppsx

... by analogy to HCV and DFV NS4B proteins. In these viruses, NS4B protein is associated with replicase proteins NS3, NS5A, and NS5B [24]. In addition, NS4B protein from HCV and DFV is membrane- associated ... mem- branes and mitochondria. Additionally, BVDV induces host membrane remodeling and these membranes con- tain BVDV NS4B protein. Finally, NS4B col...

Ngày tải lên: 12/08/2014, 04:20

... after vaccination with commercial viral vaccines containing bovine herpesvirus-1, bovine viral diarrhea virus, parainfluenza-3 virus, and bovine respiratory syncytial virus immunogens and subsequent ... 694-700. Kimman TG, Westenbrink F, Schreuder BE, Straver PJ: Local and systemic antibody response to bo- vine respiratory syncytial virus infection and re- infection in ca...

Ngày tải lên: 12/08/2014, 15:20

... soybean calcium-dependent protein kinase (CDPK) [8]. Consis- tent with this, CDPKs in guard cells are involved in Ca 2+ and anion channel activation and stomatal closure [9,10]. In line with ... because it is activated by both PMA, a well-known agonist of animal PKC, and Ca 2+ . In addition, a PKC homolog, which can be detected with the PKC antibody in Brassica juncea, is ac...

Ngày tải lên: 22/03/2014, 21:21

... electrophoresed on a 12% discontinuous SDS- PAGE gel and transferred onto nitrocellulose membranes for visualization. The membranes were washed with Tris-buffered saline (TBS) and incubated in a ... Birnaviridae, is a double-stranded RNA virus with a genome consisting of segments A and B [26]. Segment A contains two open reading frames encoding VP5 protein and a precur...

Ngày tải lên: 07/08/2014, 23:22

... distinguish between the two of them and, hence, the correct diagnosis and treatment difficult. Case presentation: A six-month-old boy from Pakistan was brought to our hospital with tachypnea and supraventricular ... lesions. The disa dvantages of this method are the larger size of the ca theter and its stiffness. We elected to use RF energy and minimized the number of catheters use...

Ngày tải lên: 10/08/2014, 23:20

... ZsI and PG conceived of the study, and participated in its design and coordination, and helped to draft the manuscript; L-MT and M-OS carried out the immunoassays; GSZ, T, GP, KH, RSZ and ZSz ... these assays is 5 ng/ml of ficolin-2 and 1 ng/ml of ficolin-3. The inter-assay coefficient of variation (CV) is 7.1% and 4.7% and the intra-assay CV 4.3% and 3.9% for the...

Ngày tải lên: 19/06/2014, 22:20

... with EF (50 μg/ml), and infected with untreated virus. Infected cells were then incubated in medium with or without EF for 6 and 8 hours and the intra-cellular amount and localization of viral ... of IV strains (human and avian), with emphasis on a human isolate of the H5N1-type HPAIV, and to evaluate the potential for emer- gence of resistant strains, in comparison w...

Ngày tải lên: 12/08/2014, 04:20

... polyprotein processing and RNA replication. The NS3 protein (69 kDa) is a multifunctional protein with an N-terminal protease domain (NS3pro) (1–180), an RNA triphosphatase, an RNA helicase and an ... Several structural (E and prM) and nonstructural proteins stimulate immunity, and the nonstructural proteins NS1 and NS3 are the dominant sources of cross-react- ive...

Ngày tải lên: 19/02/2014, 00:20

... of androgen receptor and huntingtin caused by expanded polyglutamine tracts [16,17]. Simian virus 40 (SV40) large T antigen (TAg) is a multifunctional oncoprotein which is able to induce transformation ... Lazaridis I (2000) Hsc70 interactions with SV40 viral proteins differ between permissive and non permissive mammalian cells. Cell Stress Chaperones 5, 132–138. 31 Langer T, Lu...

Ngày tải lên: 16/03/2014, 05:20

... assay on poly (dT). Lanes 1 and 2, control reaction with DNA polymerase a-primase lacking TAg or vice versa; lanes 3 and 4, 0.2 U and 0.4 U of human; lanes 5 and 6, 0.2 U and 0.4 U of murine DNA ... assistance. This work was financially supported by the Deutsche Forschungsgemeinschaft (Na190/12 and Na190/13-1) and the EC (CT970125). The IMB is a Gottfried-Wilhelm-Leibniz-Inst...

Ngày tải lên: 17/03/2014, 03:20