Báo cáo khoa học: " Cassette deletion in multiple shRNA lentiviral vectors for HIV-1 and its impact on treatment success" potx

... (manuscript submitted). We selected one 6× shRNA combination along with its series of related intermediate combinations and corresponding single shRNA vectors to test herein. This comprised shRNAs #3 (Pol 248-20), ... flanking sequence) as a self-contained expression cassette, and sequentially inserting them into a single vector via an infinitely expandable cloning strategy. The...

Ngày tải lên: 12/08/2014, 04:20

... M-protein in the serum or in the urine, and do not have monoclonal plasmacytosis of bone marrow [9]. SBP is more common in males than in females, most com- monly affects the axial skeleton, and ... HIV infection may eventually lead to clonal selection. The increased levels of interleukin 6 asso- ciated with HIV infection; and the clonal expansion of plasma cells caused by co-inf...

Ngày tải lên: 11/08/2014, 20:20

... dramatically increases, yielding 11 Proceedings of the 21st International Conference on Computational Linguistics and 44th Annual Meeting of the ACL, pages 9–16, Sydney, July 2006. c 2006 Association for ... corre- lation with human judgments. In Proceedings of Work- shop on Intrinsic and Extrinsic Evaluation Measures for MT and/ or Summarization at ACL-2005. Peter F. Brown, S...

Ngày tải lên: 20/02/2014, 11:21

... bond inhibits the monomer-dimer transformation. (A) The gel filtration chromatogram of the disulfide-containing (oxidizing) Bet v 4 mutant; the protein was incubated at 4 °C (continuous line) and ... remained in a monomeric conforma- tion. If expressed in monomeric form, Bet v 4 remained monomeric over days to weeks at 20 °C. However, after months, Bet v 4 adopted a dimeric con- formation...

Ngày tải lên: 06/03/2014, 11:20

... interests by excluding any interest that occurred within a +/-10 window (based on its position in the list) in multiple age groups. We show the top interests in each age group in Ta- ble 2. For ... compari- son of logistic regression and naive bayes. Neural Information Processing Systems, 2:841–848. Scott Nowson and Jon Oberlander. 2006. The iden- tity of bloggers: Opennes...

Ngày tải lên: 07/03/2014, 22:20

... aufhOren. 3.2. Word Formation Word Formation Rules consist of a source definition and a target definition. The source definition determines what (kind of) formatives are taken to form a new word. ... entries for aufhOren, one for the continuous and one for the discontinuous occurrences. In Word Manager, the recognition of separable verbs is entirely within the reusable le...

Ngày tải lên: 17/03/2014, 07:20

... infusion. In the HSS group, CO, CI and SV remained constant at low levels after 90 min despite the increasing maximal values of CO, CI and SV at the end of infusion, reaching 21.0 ± 6.3 l/ min ... substitute due to its higher initial increase in circulating plasma volume, its moderate duration of effect and its low incidence of anaphylactic reaction. Therefore, administration...

Ngày tải lên: 07/08/2014, 18:21

... competing interests. Authors' contributions MB and ARA were involved in the concept and design, preparation of draft and editing of final manuscript. Both authors read and approved the final ... phenomenon in their opinion. Until now, this definition of spontaneous regression, with few additions, has remained the one in most common usage today [10]. In fact, what happened...

Ngày tải lên: 09/08/2014, 04:21

... performed image fusion and screened patients for inclusion; MP served as reader; GW performed image fusion and screened patients for inclusion; GG served as reader; JR conceived of the study and ... registration. Match points were defined on corresponding landmarks such as branches of the portal vein to enable fusion of MR and planning CT/dosimetry data. Landmarks were restricte...

Ngày tải lên: 09/08/2014, 08:23

... probe, and PCR buffer containing 4.5 mM MgCl 2 . After an initial denaturation at 95°C for 5 min, 50 cycles consisting of 95°C for 15 s and 64°C for 1 min were performed. Primers and probes for ... was performed in a 25-μL total volume containing 1 μLof cDNA, 9 μL of 2.5× SYBR Green mix (Tiangen, Beijing, PR China), and 10 μmol/L primer pairs. The following cycling condition...

Ngày tải lên: 10/08/2014, 22:21