Báo cáo khoa học: "Molecular characterization of the Great Lakes viral hemorrhagic septicemia virus (VHSV) isolate from USA" pot

... of the Novirhabdovirus genus. The termini of the viral genome have conserved sequences at the 3'-end (CAUAG/UU) and 5'-end (G/AAUAUG) as other members of the Novirhabdovirus genus. The ... and coding regions of several other strains of VHSV have been deter- mined later [10]. In this study, we characterized the entire genome of the Great Lakes VHSV...

Ngày tải lên: 12/08/2014, 04:20

... in other IHNV strains. The first 15 of the 16 nucleotides at the 3’- and 5’-termini of the genome are complementary, and the first 4 nucleotides at 3’-ends of the IHNV are identical to other ... 134:207-220. 17. Ammayappan A, Vakharia VN: Molecular characterization of the Great Lakes viral hemorrhagic septicemia virus (VHSV) strain from USA. Virol J 2...

Ngày tải lên: 12/08/2014, 04:21

... of note that the structure of the LPS O-antigen of F. columnare differs structurally from the LPS O-antigen of the fish pathogen Flavobacterium psychrophilium [21], which is a linear polymer of ... However, the nature of the adhesins involved have not been identified, but possible candidates are LPS, capsule, fimbriae or other appendages of the bacterium, a hypothes...

Ngày tải lên: 17/03/2014, 10:20

... pathways. Neither endocytic (Fig. 6) nor biosynthetic transport to the vacuole (Fig. 7) is affected in the absence of Osh6p or the presence of a high level of Osh6p. The discrepancy may be due to the ... charac- terization of Osh6p, one of the seven OSBP homo- logs in yeast. We show, for the first time, a direct binding of PA by the ORD domain and the cellular loc...

Ngày tải lên: 07/03/2014, 21:20

... the blood-meal from the host is the only source of energy. Elucidation of the function of HlSCP1 in the hard tick may contribute a better understanding of the physiology of blood digestion and ... a molecular mass of 53 kDa and a pI of 7.5 (Fig. 2A), corresponding to the predicted size of the putative pro- tein calculated from the HlSCP1 amino acid sequen...

Ngày tải lên: 16/03/2014, 10:20

... Scheme of the three-hybrid strategy used in the screen. (B) Number of colonies identified in each step of the screen. (C) Distribution of the 63 distinct sequences in relation of their position in the ... elegans mRNA database, they found a high number of mRNAs that have the binding motif on their 3¢ UTR sequence. Some of the mRNAs were shown to be potential targets...

Ngày tải lên: 18/02/2014, 06:20

... between the two steps of the TES reaction The substrate guanosine to be excised (G 1 ) and its 2¢-OH group are required for the second step of TES [48], similar to (if not the same as) the role of the ... slower than the Tetrahymena ribozyme. Therefore, it appears that there is room for improve- ment in terms of the rate of reaction. This would be beneficial not so...

Ngày tải lên: 18/02/2014, 18:20

... ligase and the role of Pin1. Pin1 is brought in with the cyclin E substrate, through interaction with the pSer384-Pro motif. When the com- plex contains the CDC4a isoform, the iso- merase activity of ... for destabilizing the double bond character of the pThr-Pro bond [26]. Further evidence is available for the catalytic mecha- nism of Pin1 resembling more closely th...

Ngày tải lên: 07/03/2014, 05:20

... potential electron acceptors (Table 3). However, none of these caused an oxidation of NADH when added to the anoxic reaction mixture. Discussion The noxA-1 and the noxB-1 gene from the hyperthermo- philic ... group of small 20-kDa proteins, that form H 2 O 2 instead of H 2 O. The NADH oxidases from Thermus aquaticus and Thermus thermophilus also belong to this group [7,8...

Ngày tải lên: 08/03/2014, 02:21

... analysis of the 5 ¢ end of the xMGP promoter reveale d a minimal activating ele- ment in the s equence from )70 to )36 bp. Synthetic reporter constructs containing three c opies of the d efined ... to the right of each column. The data show a repre- sentation o f five independent experiments. Fig. 7. Binding of a nuclear factor from A6 cells to the )70/)36 region of...

Ngày tải lên: 08/03/2014, 10:20