Báo cáo khoa học: " Nested-multiplex PCR detection of Orthopoxvirus and Parapoxvirus directly from exanthematic clinical samples" pdf

... standardization and (B) sensi-tivity testsFigure 1 (A) OPV/PPV nested-multiplex standardization and (B) sensitivity tests. Exanthematic lesions from BV and CE outbreaks were used in PCR standardization and ... present work, the creation of a multiplex PCR method for the simultaneous detection of OPV and PPV has been described and tested with exanthematic clini...

Ngày tải lên: 12/08/2014, 04:20

... mentioned above and that of Gibson and Wexler (1994), Siskind (1992) Sakas and Fodor (2001), and Yang (2002). These all evaluate their learners on the basis of a small number of predefined syntactic ... A., and Navarro, D. (2009). Joint acquisition of word order and word refer- ence. In Proceedings of the 31th Annual Con- ference of the Cognitive Science Society. Pearl,...

Ngày tải lên: 31/03/2014, 20:20

... precision of 74% and recall of 60%, and K-nearest neighbors in TiMBL: both in (Evans, 2001) with precision of 73% and recall of 69%, and in (Boyd et al., 2005) with precision of 82% and recall of 71%. Since ... approach with Ferr ´ andez and Peral (Ferr ´ andez and Peral, 2000) by employing both algorithms in sequence: first Ferr ´ andez and Peral’s algorithm to det...

Ngày tải lên: 31/03/2014, 21:20

... interpretation of data, involved in drafting and revision of the manuscript, LNH Designed the study, involved in analysis and inter- pretation of data, drafted and revised manuscript. All authors read and ... subpopulations of Peripheral Blood Mononu- clear Cells. RNA from subpopulations of flow cytometry sorted cells was isolated and subjected to quantitative RT -PCR f...

Ngày tải lên: 10/08/2014, 22:20

... the presence of a heterogenous mixture of variably sized clus- ters. Furthermore, following evaporation of methanol, the majority of fullerene clusters do not reaggregate, and have a range of sizes of ... Central Page 1 of 11 (page number not for citation purposes) Journal of Nanobiotechnology Open Access Research C 60 -Fullerenes: detection of intracellular photolumines...

Ngày tải lên: 11/08/2014, 00:22

... Cleaning of gold electrodes. Immobilization of QDs on the electrode surface. Confirmation of QDs immobilization. Detection of 4-aminophenol and p- aminophenyl phosphate (pAPP). Immobilization of ALP ... Data are shown for detection of 4AP (cfg. Figure 4), pAPP (cfg. Additional File 1), and detection of 4AP after enzymatic degradation of pAPP with ALP (cfg. Figure 5)....

Ngày tải lên: 11/08/2014, 08:20

... was added to the reactions and 1 μl of a 100× dilution of the first PCR (unpurified) or 1 μl of 20× dilution of purified first PCR product was used as the template. PCR and HRM analysis were carried ... mutates G/C to A/T [26]. PCR of SSII and HRM analysis PCR primers used to amplify part of the carboxyl terminal domain of the SSII gene (GenBank accessions AB2014...

Ngày tải lên: 12/08/2014, 03:21

... proved useful in the detection of several RNA viruses [11]. Aim of this study was first to evaluate the suitability of IHC for detection of IBV antigen in paraffin- wax embedded CAM and second to analyse ... slow and time consuming. Currently, detec- tion and serotype analysis of IBV is performed by RT -PCR and restriction fragment length polymorphism analysis [13] or b...

Ngày tải lên: 12/08/2014, 04:21

... Annals of Clinical Microbiology and Antimicrobials 2011, 10:33 http://www.ann-clinmicrob.com/content/10/1/33 Page 2 of 5 RESEARCH Open Access Rapid PCR detection of group a s treptococcus from flocked ... article as: Slinger et al.: Rapid PCR detection of group a streptococcus from flocked throat swabs: A retrospective clinical study. Annals of Clinical Microbiol...

Ngày tải lên: 12/08/2014, 17:20

... Page 2 of 3 (page number not for citation purposes) Critical Care Vol 10 No 2 Struelens and Denis endemic MRSA in The Netherlands and Scandinavia [5,6]. Clinical practice recommendations ... hours for presumptive MRSA detection [8]. During this delay of several days between sampling and reporting of detection results, MRSA carriers may constitute a source of cross-coloniza...

Ngày tải lên: 12/08/2014, 23:22