Báo cáo khoa học: " Reverse genetic characterization of the natural genomic deletion in SARS-Coronavirus strain Frankfurt-1 open reading frame 7b reveals an attenuating function of the 7b protein in-vitro and in-vivo" docx

... Journal Open Access Research Reverse genetic characterization of the natural genomic deletion in SARS-Coronavirus strain Frankfurt-1 open reading frame 7b reveals an attenuating function of the 7b ... apoptosis, including the 7a and 7b proteins [18,46,47]. To analyze whether the deletion in ORF 7b had any influence on its Amino aci...

Ngày tải lên: 12/08/2014, 04:20

... Journal Open Access Research Reverse genetic characterization of the natural genomic deletion in SARS-Coronavirus strain Frankfurt-1 open reading frame 7b reveals an attenuating function of the 7b ... be involved in the induction of apoptosis, including the 7a and 7b proteins [18,46,47]. To analyze whether the deletion in ORF...

Ngày tải lên: 12/08/2014, 04:20

... electrophoresis. Another striking difference of the MALDI-TOF spec- tra from the modified Fe -protein in comparison to the unmodified Fe -protein is the decreased intensity of peak 1625.4 m ⁄ z and the absence of ... mutagenesis of the target arginine residue R102 on modification of the NifH protein. Western blot analysis of Azoarcus wild-type strain BH72 (lane...

Ngày tải lên: 07/03/2014, 02:20

... ricin B-chain Leu152; and Met234 of the ML1p and ML2p B-chains corresponding to the ricin B-chain Leu233). Estimation of the quantitative ratio of the three mlp gene variants in genomic DNA and ... variants, and this will result in an overestimation of the amount of genomic DNA containing one copy of the mlp gene. Nevertheless, taking into account th...

Ngày tải lên: 07/03/2014, 15:20

... His290 and Asp260 e merge from the b8-a8andb7-a7 loops, respectively. Another important feature of the catalytic machinery is the so-called oxyanion hole necessary for the stabilization of the oxyan- ion ... inserted into t he expression vector following the manufacturer’s instruction (Gateway, Invi- trogen). The correct insertion of the Rv1399c ORF was confirmed by DNA...

Ngày tải lên: 07/03/2014, 16:20

... family and contains a di-zinc binding domain and a small domain that is inserted in the middle of the metal- binding domain and mediates catalysis. The di-zinc Fig. 6. Metal effects on the enzymatic ... bound in an ‘oxyanion- binding hole’ formed by Zn1 and the imidazole group of His219 in PepD (corresponding to H269 in PepV), resulting in the polarization...

Ngày tải lên: 23/03/2014, 06:20

... embryonic RNA using combinations of degenerate and specific primers and by rescreening the cDNA libraries. The partial clones of XHIVEP2 and -3 covered 5.9 and 3.2 kb of the respective 3¢ ends and included ... specific dominant negative and constitutively active constructs by the generation of deletion mutants containing discrete functional domains of XHIVEP1. Th...

Ngày tải lên: 30/03/2014, 13:20

... mean (n = 3). Expression and characterization of the flavoprotein domain of gp91phox 25 2. Biberstine-Kinkade KJ, Yu L, Dinauer MC. Mutagenesis of an arginine- and lysine-rich domain in the ... p67 phox in activating diaphorase activity of the flavoprotein domain either by binding to p67 phox , producing an active conformation, or by binding simul- taneously to...

Ngày tải lên: 07/08/2014, 14:22

... rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein- dye binding. Ann Biochem 1976, 72:248-254. 56. Watanabe N, Lam E: BAX inhibitor-1 ... candidate cDNAs for NDR1 andanalyzedthededucedprimary amino-acid sequences. Domain conservation and the high degree of homology between the coffee proteins and AtN...

Ngày tải lên: 11/08/2014, 11:21

... Veterinary Medicine of Sichuan, Agricultural University, Ya&apos ;an, Sichuan, 625014, PR China and 2 Key Laboratory of Animal, Diseases and Human Health of Sichuan Province, Ya&apos ;an, Sichuan, 625014, ... at 4 μm and stained with an indirect immunofluorescent technique. Labels on the left side of the images indicate organs. Negative control is shown in the left c...

Ngày tải lên: 12/08/2014, 04:21