Báo cáo y học: "Reverse genetic characterization of the natural genomic deletion in SARS-Coronavirus strain Frankfurt-1 open reading frame 7b reveals an attenuating function of the 7b protein in-vitro and in-vivo" pptx

... characterization of the natural genomic deletion in SARS-Coronavirus strain Frankfurt-1 open reading frame 7b reveals an attenuating function of the 7b protein in- vitro and in- vivo Susanne Pfefferle 1 , ... SARS-CoV accessory gene products have been shown to be involved in the induction of apoptosis, including the 7a and 7b protein...

Ngày tải lên: 12/08/2014, 04:20

... Journal Open Access Research Reverse genetic characterization of the natural genomic deletion in SARS-Coronavirus strain Frankfurt-1 open reading frame 7b reveals an attenuating function of the 7b ... SARS-CoV accessory gene products have been shown to be involved in the induction of apoptosis, including the 7a and 7b proteins [18,46,4...

Ngày tải lên: 12/08/2014, 04:20

... NICN1,wehavenow analyzed the human and murine orthologs and provide initial data on the expression profiles and cellular localiza- tion of the protein product. MATERIALS AND METHODS General methods Standard ... of the cDNAs in the used panels is normalized to four different housekeeping genes. The PCR analyses of the panels was carried out according to the man...

Ngày tải lên: 23/03/2014, 21:20

... participated in the design of the study and analysis of the data. FC participated in the design of the study and in the anal- ysis of data and helped to draft the manuscript. PM cloned and sequenced ... revision of the study. AS partic- ipated in the analysis and interpretation of data and helped to draft the manuscript. RR participated in...

Ngày tải lên: 09/08/2014, 08:22

... FcγRIIb staining was observed in the synovial lin- ing and sub-lining layers, perivasculary and inside lymphocyte infiltrates (e). (d) No staining of the RA synovium was observed when the anti- FcγRIIb ... very similar and both receptors were often found perivasculary and always in the synovial lining and sub-lining layers of the synovium. FcγRII and III positiv...

Ngày tải lên: 09/08/2014, 10:20

... information, the fraction of total RNA that delivers coding information to the protein- synthesizing machinery (i.e. mRNA ) is in trinsically labile and continuously synthesized. The steady-state level of mRNA ... vary and is a function of RNA synthesis and decay, the control of mRNA stability plays an essential role in the regulation of gene expression. As...

Ngày tải lên: 10/08/2014, 05:21

... protein as judged by SDS/ PAGE. The activity of the pure recombinant protein was 80% of that of t he wild-type protein in both the lysozyme and chitinase assays. Attempts to further purify the recombinant ... sp. [16] and B. circulans [33] chitinases, where the Asp fi Asn mutants retained only 0.03% and 0.2% of the wild-type activity, respectively. This sugges...

Ngày tải lên: 22/02/2014, 04:20

... cysteine residues: the 2Cys-Prx subgroup, and 1Cys-Prx subgroup contain two and one conserved cysteines, respectively. 2Cys-Prx proteins are reduced by the AhpF protein in bacteria, and by the ... proteins in databases. Tryptic peptides were puri®ed by RP-HPLC and some of them were to tally or partially analyzed by Edman sequencing (ááá) and/ or by MALDI-TOF mass spectr...

Ngày tải lên: 08/03/2014, 16:20

... with MEGALIGN program), and 65% are conserved using BLAST analysis comparing all of the vitamin K-dependent carboxylases. In contrast, the C-terminal 25% of the protein shows no homology to any of the other vitamin ... Using the bovine numbering system (Fig. 1), we observe Arg179 rather than histidine, Thr430 rather than alanine, Pro654 rather than serine, Met726 rather...

Ngày tải lên: 17/03/2014, 10:20

... contraction by activating ryanodine receptors of the sarcoplasmic Fig. 1. Isolation of tigrin from the Duvernoy’s glands of R. tigrinus tigrinus. (A) The extract from Duvernoy’s glands of R. tigrinus ... [51,52]. Antigen 5 proteins are components of the venoms of wasps and ants, and they are thought to be involved in the provocation of an acute and localized...

Ngày tải lên: 18/03/2014, 01:20