Báo cáo y học: " Herpes simplex virus UL56 interacts with and regulates the Nedd4-family ubiquitin ligase Itch" pps

... study demonstrates that HSV-2 UL56 interacts with t he Nedd4-family ubiquitin ligase Itch, and more- over, targets Itch for degradation primarily via the lysosome pathway and partially via the ubiquitin- protea- some ... Itch may be degraded by the lysosome pathway and in part by the proteasome pathway in HSV-2-infected cells. The decrease of Itch in Vero-GFP -UL5...

Ngày tải lên: 12/08/2014, 04:20

... of pregnancy until delivery, significantly reduces the fre- quency of clinical manifestations and the virus shedding at the time of delivery decreasing the need for caesarean delivery and probably the ... characterized by the presence of antibody against the same HSV type and the herpes outbreaks are usually mild (7–10 days) with less severe symptoms than the fir...

Ngày tải lên: 12/08/2014, 04:21

... reaction analyses for herpes simplex virus, herpes zoster virus, enterovirus, Epstein Barr Virus and cytomegalovirus (CMV) were negative. The suspected diagnosis was AIDP, part of the heterogeneous ... this topic and highlights the need for further study. Abbreviations HIV: Human immunodeficiency virus; GBS: Guillain-Bar- ré syndrome; AIDP: Acute inflammatory demyelinatin...

Ngày tải lên: 11/08/2014, 19:21

... The ORF of UL56 and UL56AY, mutant UL56 with all three PPXY motifs mutated to AAXY (P23A, P24A, P49A, P50A, P145A, P146A), were PCR amplified from pcDNA -UL56 and pcDNA-UL56AY, respectively, and ... pro- tein 0 of herpes simplex virus 1 dynamically interacts with proteasomes, binds and activates the cdc34 E2 ubiquitin- conjugating enzyme, and possesses in vitr...

Ngày tải lên: 12/08/2014, 04:20

... from human laryngeal carcinoma [38]. Therefore, to further increase the ability of the OncSyn virus to fuse all types of cells, we generated the OncdSyn virus carrying both the gBsyn3 and gKsyn1 mutations. ... the fusogenicity of the new virus OncdSyn. The OncdSyn virus reduced primary tumor sizes and inhibited metas- tases to distal organs in the 4T1 syngeneic mou...

Ngày tải lên: 20/06/2014, 01:20

... monocytic (U937) cell cultures were infected with wild type herpes simplex virus type 1 (HSV-1) (F), the Us3 deletion virus (R7041) [42], its repair virus (R7306) [42], the Us3 and ICP4 deletion virus ... infections and the statistical analyses, and drafted the manuscript. RKM participated in the PCR and protein assays. HK participated in the PCR assays. EB,...

Ngày tải lên: 20/06/2014, 01:20

... nervous system infection with herpes simplex virus (HSV) is important because of the potential morbidity and mortality associated with the disease as well as the wide availability of acyclovir ... electroencephalogram; ELISA: enzyme linked immunosorbent assay; HIV: human immunodeficiency virus; HSV: Herpes Simplex Virus; HSVE: Herpes Simplex Virus Encephalitis;...

Ngày tải lên: 11/08/2014, 19:21

... outcome. The pathogenicity of the virus therefore remains unknown, and the rare association in the critically ill of HSV-1 isolation with mortality may represent reactivation of the virus in immunodepressed ... HSV-1, rather than the presence of a primary and damaging infection. Indeed, critically ill patients with sepsis may have late immunodepression, with lymphocy...

Ngày tải lên: 12/08/2014, 20:20

... activity was also examined by luciferase assay (b) and the level of Flag-tagged HBc protein was analyzed by Western blot using an anti-Flag antibody (d). In the stably-transfected Huh7 cells, the ... particles were detected by real-time PCR analysis as previously described [16]. The culture supernatant and the cy toplas mic lysate of the transfected cells were digested with...

Ngày tải lên: 12/08/2014, 01:22

... some of the experiments and drafted the manuscript. KM participated in the design of the study and analysed the results. PP, RF, KN and KM constructed the expression vectors and produced and purified ... indi- viduals with HCV genotype 3a and two individuals with genotype 1b infection were followed-up during and after IFN-α monotherapy. In genotype 1 infected pat...

Ngày tải lên: 12/08/2014, 04:21