Báo cáo y học: " Peptides derived from the HIV-1 integrase promote HIV-1 infection and multi-integration of viral cDNA in LEDGF/p75-knockdown cells" doc

... stimulate the enzymatic activity of the IN itself in vitro, and consequently the integration of viral cDNA in virus infected cells [25]. In the current work we show that in the presence of the INr and ... appears that binding of the IN to the LEDGF/p75 protein is also mediated by the same do main [2]. It was therefore of interest to determine wh...

Ngày tải lên: 12/08/2014, 04:20

... used annexin V/PI flow cytometry analysis to quantify the viable, necrotic and apoptotic cells. The expression of cyclin E and cyclin B1 was determined with annexin V/PI flow cytometry and western ... indicated that cyclin E was more impor- tant than cyclin B 1 in CPT-induced apoptosis in the HL- 60 cells. Cyclin E is essential in the cell cycle from G 1 to S- phase...

Ngày tải lên: 13/08/2014, 15:21

... neuroprotection. Competing interests The authors declare that they have no competing interests. Authors' contributions GMX and WQY participated in the trial design and were involved in the study analysis and ... Ltd, Tianjing, China) or matching placebo within 8 hours of the documented time of injury. Qualifying patients were randomly assigned in a 1:1 manner to r...

Ngày tải lên: 13/08/2014, 11:22

... in spite of all these difficulties, the topic of reverse engineering gene networks is surely worth pursuing, as it provides us with a means of understanding biology not only in terms of the genes themselves, ... inferred from keloid data, suggesting a more robust network in the keloid condition. The ability to infer molecular interactions in cellular systems is one...

Ngày tải lên: 13/08/2014, 16:20

... offset of the T wave, then the offset of the QT interval was defined as the intersection of the tan- gent to the midpoint of the downslope of the T wave and the isoelectric baseline. Cardiologists ... QT interval. In the Bazett method, the QT interval was divided by the square root of the R-R interval (defined as the length of the entire cardiac...

Ngày tải lên: 13/08/2014, 18:21

... dressings or early excision and grafting is the key to avoid burn wound infection and its extension to systemic infection [8-10].  e timing and extent of surgery may vary, as well as the method ... using more physiologic endpoints such as urinary output and other measurements, so the trend in burn resuscitation is shifting the focus from fl uid formulas to adequ...

Ngày tải lên: 13/08/2014, 20:21

... sequencing A physical map of the porcine genome was constructed using the fingerprints and end sequences generated from over 264,000 BACs from 4 BAC libraries and ordering information derived from ... low-coverage sequencing on the structure of the assembly is considerable. Reducing the number of sequence reads from a depth of 7.5× to 5× and 2.5× increases t...

Ngày tải lên: 14/08/2014, 08:20

... matter of the spinal cord; contains the rubrospinal tract. En passage Within a pathway. Gray matter CNS tissue containing the majority of neuron cell bodies and a relatively low density of myelin. ... cells with the phenotype of type-2 astrocytes, potentially contaminating cell types were removed from the suspen- sion by immuno-panning with the anti-A2B5 antibody. A sm...

Ngày tải lên: 06/08/2014, 18:21

... pregnancy is surgery by laparoscopy or laparotomy depending upon the condition of the patient. The main aim of the surgery should be the preservation of the intrauterine pregnancy with minimal manipulation ... techniques. Conclusion Heterotopic pregnancy can occur in the absence of any predisposing risk factors, and the detection of an intrauterine pregnancy does...

Ngày tải lên: 11/08/2014, 14:20

... actually not covered by these guidelines, and there are no other indications on the management of these patients in the literature. The estimated annual incidence of MI, new and recurrent in the ... considered the low age and the low cardio- vascular risk profile of the patient at the time of acute MI, the absence of symptoms after the event and the...

Ngày tải lên: 11/08/2014, 19:21