Báo cáo y học: "Cloning, expression and characterization of gE protein of Duck plague virus" doc

... Academy of Sciences, Beijing, China A nitroalkane-oxidizing enzyme gene (naoA) was cloned from a genomic DNA library of Streptomyces ansochromo- genes 7100. The deduced protein (NaoA) of this gene ... 6303 Cloning, expression and characterization of a gene encoding nitroalkane-oxidizing enzyme from Streptomyces ansochromogenes Jihui Zhang, Wenbo Ma* and Huarong Tan Institute...

Ngày tải lên: 31/03/2014, 08:20

... purification, and production of polyclonal antibody. Intervirology 2009, 52:141-151. 12. Liu FY, Ma B, Zhao Y, Zhang Y, Wu YH, Liu XM, Wang JW: Characterization of the gene encoding glycoprotein C of duck ... usually consist of a tetrapeptide bearing the sequence YXXØ (Y is tyrosine, X is any amino acid, and Ø represents any hydrophobic res- idue) [36,37]. The DPV gE p...

Ngày tải lên: 12/08/2014, 04:20

... amount of enzyme necessary to form 1 lmol of indole pyruvate, q-hydroxyphenylpyruvate or phenylpyruvate. A paper chromatography assay for amino acids was also used to detect the activity toward tryptophan. ... for the expression of protein R40 3Y; T7 promoter-based expression vector; Km r Present study a Key Laboratory of Monitoring and Management of Crop Diseases and Pest...

Ngày tải lên: 14/03/2014, 23:20

... the cel mix. Analysis of the hydrolysate by TLC showed glucose and cellobiose to be the Fig. 3. MS analysis of the products of tamarind seed xyloglucan (tam- XG) hydrolysis by xyloglucan-specific ... endoglucanase activity. The nature and presence of a CBM varies among the family-74 proteins: four of 13 family-74 genes code for a family-2 CBM; three have a family-1 fungal CBM; on...

Ngày tải lên: 23/03/2014, 21:20

... Regions of homology with proteins of other species are underlined and the percentage of amino acid identity is indicated. (B) Schematic representation of the human genomic structure of p76 RBE gene. ... and proteins were extracted and affinity purified on glutathione agarose beads (Sigma, Bornem, Belgium) by the method previously described by Frangioni [26]. Purity and integri...

Ngày tải lên: 23/03/2014, 21:20

... Division of Pathology, Faculty of Veterinary Medicine, Utrecht University, The Netherlands and 3 Department of Infectious Diseases and Immunology, Division of Virology, Faculty of Veterinary Medicine, ... gel electrophoresis, and sequencing were used to examine each sample for purity and specifi- city. For each experimental sample, the amount of the genes under study, a...

Ngày tải lên: 13/08/2014, 13:20

... at the Peptide Synthesis and Analysis Laboratory of the Department of Medical Biochemistry and Microbio- logy, Uppsala University. The SP6 primer 5¢-ATT TAG GTG ACA CTA TAG-3¢ and the three oligonucleotide PCR-primers ... embryonic kidney cell cDNA- library followed by expression and purification, yielded a protein with a molecular mass of 13 700 Da, and an EDTA-insensitive...

Ngày tải lên: 21/02/2014, 01:21

... correlate exactly with the mobilities of di-, mono- and unglycosylated forms of recombinant cystatin F previously characterized by enzymatic removal of N-linked carbohydrate and carbohydrate composition analysis ... washed and mounted as above. Quantitative protein assays Cell lysates and culture media were analysed by ELISA to determine the concentrations of cystatin F [5]...

Ngày tải lên: 21/02/2014, 01:21

... stationary phase, which is accompanied by a variety of physiological changes that affect gene expression, the structure and composition of the cell wall, DNA organization, synthesis of storage compounds ... itself (Fig. 2), and one larger protein consisting of two UspA domains in tandem [17]. The small members of this family are proteins of 142–144 amino-acids long; they ar...

Ngày tải lên: 22/02/2014, 07:20

... aused by SAgs a re food poisoning and toxic shock syndrome ( TSS) [4,5]. SAgs produced by several strains of Staphylococcus aureus and Streptococcus pyogenes are structurally and immunologically the ... have one Cys (SEI, SEK and SPEC), two have three Cys (SEG and SPEA) and only SSA has more than that, five Cys. As discussed later, the fact that SSA has two Cys r esidues (Cys26...

Ngày tải lên: 07/03/2014, 16:20