Báo cáo y học: "Interactions of SARS Coronavirus Nucleocapsid Protein with the host cell proteasome subunit p4" ppt

... with the proteasome subunit p42 within host cells. These data enhance our understanding of the molecular mechanisms of SARS- CoV pathogenicity and the means by which SARS- CoV interacts with host ... primarily by the cellular immune response and cytokines. SARS- CoV nucleocapsid protein (SARS- CoV NP) is an extensively phosphorylated, highly basic, vital struct...

Ngày tải lên: 12/08/2014, 04:20

... ribosomes. Reconstitution of synthesis using all of these homoge- neous proteins revealed that they are insufficient for synthesis directed by a native mRNA and that several other proteins are required [1–6]. One of these ... precursor of hypusine in the eIF-5A protein [32]. The model in Fig. 5, in which the orientation of the spermidine residue of EF-P is facing the mR...

Ngày tải lên: 16/03/2014, 06:20

... completely destroyed the protein function (Fig. 4A and 5). Furthermore, the results of the cross-link- ing assay confirm ed that all N- and C- terminal dele- tions severely affected the ability of N protein ... domains and then by introducing point mutations of amino acid residu es. Mutagenesis strategy was based on the computer modeling of secondary and tertiary structu...

Ngày tải lên: 12/08/2014, 04:20

... University School of Medicine, Department of Cardiology, Antalya, Turkey; 2. İnönü University School of Medicine, Department of Cardiology, Malatya, Turkey; 3. Erciyes University School of Medicine, ... Department of Cardiology, Kayseri, Turkey; 4. Başkent University School of Medicine, Department of Cardiology, Adana, Turkey; 5. Başkent University School of Medicine, De...

Ngày tải lên: 26/10/2012, 08:57

... episodes may be difficult (5,6,7,8). Autonomically mediated forms of reflex syncope (such as neurocar- diogenic or vasovagal syncope) may produce sudden episodes of profound hypotension and bradycardia ... adequate therapy pursued. In all three pa- tients the presumed mechanism of the observed pe- riods of asystole was neurocardiogenic in nature. These findings would be consis...

Ngày tải lên: 26/10/2012, 09:53

... membrane proteins will then be reflected by the activity of these reporter gene products, which can be measured similarly to the conventional yeast two hybrid system. The bait protein, which is the ... NubG domain. The fusion plasmids are then cloned into the yeast cell and expressed on the cell membrane. If the prey protein interacts with the bait protein,...

Ngày tải lên: 02/11/2012, 11:08

... the (2–5)A pathway is also induced by interferons [15]. The major enzyme in this pathway, the (2–5)A synthetase catalyzes the synthesis of a series of 2¢)5¢- linked oligoadenylates, termed (2–5)A ... identified by thin-layer chromatog- raphy, immunologically and by high-performance liquid chromatography. The biological activity of (2–5)A oligo- mers was verified by inhibition o...

Ngày tải lên: 21/02/2014, 15:20

... flexibility f or the putative ligand in the active site region. This relaxation, together with the lack of steric h indrance created usually by the hydroxyl g roup of Ser146 in the w ild-type enzyme, results ... likely, that the lack of a hydroxyl group of Ser146 diminishes the role of this residue in the stabilization of the substrate– ligand molecule, leading...

Ngày tải lên: 08/03/2014, 23:20

... beyond the b ifurcation. The velocity of the trajectory tends to zero as it approaches this steady state (exactly a t this point, the velocity is zero). Therefore, the period of t he limit cycle ... even in the absence of an exchange across the plasma membrane and of a n intrinsic dynamics of the IP 3 R [113]. In Ca cyt /protein models, the role of alternating...

Ngày tải lên: 24/03/2014, 00:21

... small dots on the cell surface. On the contrary, a transmembrane-type MFGM glycoprotein, butyrophilin [27,28], expressed in COS-7 cells was detected evenly the whole surface of the cells (Oshima, ... vesicles. In the in vitro assay system, on the other hand, DC1 lacking the a bility to a ssociate with the cell surface a nd the exosome- like membrane vesicles showed...

Ngày tải lên: 24/03/2014, 03:21