Báo cáo y học: " An efficient in vitro-inoculation method for Tomato yellow leaf curl virus" ppsx

... The results obtained showed the feasibility to inoculate effi- ciently in vitro tomato plants with TYLCV and typical symptoms of TYLCD with leaf curling and yellowing were observed. For symptom development, ... resistance against TYLCV in wild tomato genotypes using in vitro cultures. Discussion In this study, an efficient in vitro-inoculation method for TYLCV was...

Ngày tải lên: 12/08/2014, 04:20

... using a MYCN-specific antibody in three different NB cell lines, Kelly, IMR5 and WAC2. To assess whether transcripts from this region are actively transcribed and elongated, we additionally analyzed histone ... was performed using SYBR Green I detection chemistry (Eurogentec, Seraing, Belgium) on a LightCycler480 (Roche, Basel, Switzerland). Primer sequences for MYCN binding sites in th...

Ngày tải lên: 14/08/2014, 21:20

... number of viral sequences included in the analyses (NSI) and the number of viral variants (VV) -the latter including dominant and subdominant viral variants– together give an indirect measure of Table ... for strand loading to RISC complex and for a predicted silencing efficiency score, which could be used in combination for achieving over 90% silencing. Resistant and naïve isolat...

Ngày tải lên: 11/08/2014, 21:21

... potent lipooxygenase inhibitor. The results observed in a preliminary trial suggest a highly intriguing clinically relevant symptomatic effect contrasting with a poor bioavailability of this compound. ... the disease (for example, targeted therapies against cytokines such as tumor necrosis factor (TNF), interleukin-6, and so on) or against some specific enzyme (for example, cyclooxygenase...

Ngày tải lên: 09/08/2014, 11:20

... USA) and Ken Wolfe (Smurfit Institute of Genetics, Dublin, Ireland) gave two examples of how augmenting current methods with additional information (protein-protein interaction and synteny data, ... the ‘Quest for Orthologs’ meeting at the Wellcome Trust Conference Centre in Hinxton, UK in July 2009, to jointly address these issues by bringing together for the first time key re...

Ngày tải lên: 09/08/2014, 20:20

... categories and memos were coded into a word document which was refined and elaborated in light of incoming data and analysis employing an inductive stance. The interviewer conducted the analysis ... all interviews. In addition, each interview was separately analyzed by at least one other researcher to check for reliability of coding. Findings and themes were discussed regularly by...

Ngày tải lên: 11/08/2014, 16:20

... for CVB3, and up to 240 min pi for the slower replicating and highly guanidine-sensitive HRV14 (Fig. 4D). The guanidine insensitive HRV1A and 16 remained rather unaffected by guanidine in the time course ... assay in normal human lung airway cells. (A) Example images of WI-38 non- transformed primary human embryonic diploid airway cells inoculated with the indicated HRV and CV serotyp...

Ngày tải lên: 12/08/2014, 01:22

... applied for gel electropholesis. Cyp A (upper panel) and CA (lower panel) were visualized by Western blotting (WB) using an anti-CypA antibody and serum from a SIV- infected monkey, respectively. Kono ... Perron MJ, Yang PC, Stremlau M, Sodroski J: Binding and susceptibility to postentry restriction factors in monkey cells are specified by distinct regions of the human immunodeficiency...

Ngày tải lên: 13/08/2014, 01:20

... sources for visualizing, inferring the topologies of, or understanding the dynamics of networks and subnetworks. Using genotype information, gene expression, protein-protein interaction, protein phos- phorylation ... tested successfully on both synthetic glycans and glycan data from the KEGG GLYCAN database, accessed from [http://www.genome.jp/kegg/glycan]. Eugene Fratkin (Stanford Universi...

Ngày tải lên: 14/08/2014, 17:22

... think that this two-stage analysis (extracting the terminology of the domain with a robust superficial analysis, and analysing the texts with a more in- depth parser using this terminology), ... terminology will be performed in two ways : on the one hand, by a structural analysis of the terminological noun phrases extracted by LEXTER; on the other hand, by an analysis of the sent...

Ngày tải lên: 09/03/2014, 01:20