Báo cáo y học: "Characterization of the HIV-1 integrase chromatin- and LEDGF/p75-binding abilities by mutagenic analysis within the catalytic core domain of integrase" ppt

... support the hypothesis that IRF8 and NCoA3 factors may be involved in the control of the HIV latency. Western blot analysis of NCoA3 and IRF8 proteins expres-sionFigure 7 Western blot analysis of ... by recruitment of additional cofactors with HAT activity (such as p300/CBP and P/CAF) and by an intrinsic HAT activity [40] and may thus contribute to the transc...

Ngày tải lên: 13/08/2014, 09:21

... on the displaced strand of the D-loop allows lagging strand synthesis (panel A). In bubble-migration synthesis, lagging strand synthesis does not occur, and the newly synthesized single strand ... 2B). The net result is that a newly synthesized single-strand copy is created and then quickly extruded from its template, and lagging-strand synthesis does not occur within the...

Ngày tải lên: 11/08/2014, 21:21

... calibrated with standard buffers at pH 4.0 and 7.0, respectively. Flow cytometry analysis The competitive effect of Yb-Tf species on binding of Fe 2 -Tf to K562 cells was studied by flow cytometry. The solutions with ... iron requirement and subsequently express much higher Tf receptors [39]. Then the uptake of Yb-bound Tf by the tumor cells might be much higher than t...

Ngày tải lên: 08/03/2014, 09:20

... normal function of AEC by a variety of mechanisms. Some of these include decreases in the level of exhaled NO, enhanced release of pro-inflammatory cytokines, and inhibition of the airway repair process ... IgG1 and IgG2a (DAKO) were used as negative controls for iNOS and eNOS antibodies, respectively. Cell viability and cytotoxicity Viability and cytotoxicity were...

Ngày tải lên: 12/08/2014, 18:22

... succession of two types of stimuli, one presented regularly and the other displayed sporadically (Figure 2). Upon the presentation of the rare stimulus following a string of standard stimuli, the ... frequency glide, were sporadically interspersed by a stimulus which deviated from the standard in the intensity of the first component and the glide directio...

Ngày tải lên: 02/11/2012, 11:08

... hexa- methyldisilazane-trimethylchlorosilane-pyridine), and the resulting mixtures of methyl glycosides were analyzed on GLC [14,15]. The absolute configurations of the monosac- charides were determined by GLC analysis of the trimeth- ylsilylated ... composition of the EPS produced by S. thermophilus 8S, the genetics and biochemistry of the EPS biosynthesis as...

Ngày tải lên: 08/03/2014, 09:20

... physiological role for these two isoforms and, although they share basic properties, the large variation in catalytic activity for both phenolic and dyes supports this idea. The C30 laccase isoforms ... plants, they may mainly play a role in lignification [4] whereas in fungi they probably play the opposite role, i.e. delignifica- tion [5,6], among many others [1]. C30 is a white-rot...

Ngày tải lên: 08/03/2014, 09:20

... Moreover, the domain may consist of a b-sheet and a b-turn on the secondary protein structure-prediction (Fig. 7C). Based on the analysis, the domain (670–700 from the N-terminal) may exist on the ... protein may contribute to the stabilization of p53 and prevent the potential induction of apoptosis by p53. On the contrary, localization and expression time...

Ngày tải lên: 17/03/2014, 10:20

... facilitates the electrophilic attack at the methyl- ene moiety of MIO by decreasing of the electron density in the p system of the C¼C double bond (Fig. 5). Calculation of the UV spectra of MIO and the ... in binding and orienting of the imidazolyl moiety of L-histidine at the active site (Fig. 2A) and stabilization of the cationic intermediate aris...

Ngày tải lên: 18/03/2014, 01:20

... evolution, PhD Thesis, University of Tromsø, Norway. 47. Oustinova, I. (2000) Molecular phylogeny of the family Ankist rode sma ceae (Chlorophy ta, Chlorophycea e) and ana lyse s of their group ... insertion of the group I intron by replication into the intron-less site. The endonucleases I-PpoIandI-DirI from nuclear group I introns Ppo.L1925 and Dir.S956-1 in the myxo...

Ngày tải lên: 18/03/2014, 01:20