báo cáo khoa học: " The Puf family of RNA-binding proteins in plants: phylogeny, structural modeling, activity and subcellular localization" potx

... and involves the activity of an exten sive array of RNA-binding proteins. Throughout the lifetime of an mRNA, a d ynamic association exists between mRNAs and RNA-binding proteins. These interactions ... 10:44 http://www.biomedcentral.com/1471-2229/10/44 Page 9 of 19 RESEARC H ARTIC L E Open Access The Puf family of RNA-binding proteins in plants: phylo...

Ngày tải lên: 12/08/2014, 03:21

... increasing the basic nature of the domain. The precise role of the ROQ domain, and ⁄ or the CCCH motif, in contributing to RNA binding remains to be determined in future detailed structural and ... with the various motifs of Roquin, and by selectively deleting domains from the full-length protein, we determined that the ROQ domain in the N-terminus of...

Ngày tải lên: 15/03/2014, 11:20

... chains. First, the chains are degraded by both endochitinases, that attack the chitin chain randomly, and exochitinases, that attack the chitin chains from either the reducing or nonreducing ... of insoluble substrates. Bound protein was determined indirectly by measuring the concentration of free protein in the supernatants after 24 h of incubation. L. lactis chitinase...

Ngày tải lên: 18/02/2014, 08:20

... describing the details of protein–RNA interactions in the 50S subunit, Klein et al. argue that proteins joining the growing complex early during assembly (‘early proteins ) must be those with the ... many of the inter- dependencies of the individual ribosomal proteins dur- ing assembly and showed that in each subunit some proteins functioned as ‘initiators’ of...

Ngày tải lên: 19/02/2014, 17:20

... (without Collins) are given in the first lines. # is the size of the test set. The baselines are 73.1% (RC) and 51.4% (PP). The combined method performs better for sm all training sets. There is no ... co-training for statistical parsers. In Workshop on the Con- tinuum from Labeled to Unlabeled Data in Ma- chine Learning and Data Mining, ICML. Mark Johnson and Stefan...

Ngày tải lên: 20/02/2014, 12:20

... imaging assay, TMA Multistain. This allows the software to distinguish positive tumor areas within indi- vidual cores of the TMA slide. Both staining intensity and its relative area within a ... within the evaluated area of the specimen. This allowed the software to automatically quantitate not only the average intensity of each category, but also the relative area of...

Ngày tải lên: 09/08/2014, 09:22

... becoming clearer - and thus the risk of non-AIDS related complications such as cardiovascular diseases and non AIDS-defining cancers is a major contributor of the morbidity in HIV-infected individuals ... supportive of health system strengthening [38]. As coverage of antiretroviral therapy increased, so the broader benefits of ART became apparent. In Malawi, adult mort...

Ngày tải lên: 11/08/2014, 14:21

... in paraffin. They were then cut at 5µm and stained with haematoxylin and eosin for histological examination. The right side of the heart was incised and this incision extended along the Published: ... in Fennoscandian badgers is described. Routine parasitological examination of nine Norwegian badgers, at the National Veterinary Institute during 2004 and 2005, identifie...

Ngày tải lên: 12/08/2014, 18:21

... those of other then known families of cysteine pro- tease inhibitors. Clitocypin inhibits papain, cathepsins L and K, legumain and bromelain, but is inactive against cathepsin H, trypsin and pepsin. ... inhibitors of papain, cathepsin L and cathepsin V, with a mean K i value of 0.5 nm. Macrocypin 4 was also an effective inhibitor of papain but exhibited weaker inhibition of...

Ngày tải lên: 16/03/2014, 02:20

... proapoptotic member of the Bcl-2 family. However, unlike in other members of the Bcl-2 family, the BH3 domain of BNIP3 is not required for its death-inducing activity. Our results showing that full-length, ... present in striatal areas adjacent to the site of injection (Fig. 1A). High levels of BNIP3 immuno- staining were found in the cytoplasm of striatal neuro...

Ngày tải lên: 22/03/2014, 17:20