báo cáo khoa học: " Deep sequencing identifies novel and conserved microRNAs in peanuts (Arachis hypogaea L )" pps

... regulatory roles in plants. Annual Review of Plant Biology 2006, 57:19-53. 9. Zhang BH, Wang QL, Pan XP: MicroRNAs and their regulatory roles in animals and plants. Journal of Cellular Physiology ... DCL1 is a key enzyme in miRNA biogenesis and mutating this gene results in globally decreased miRNA levels in plants, ultimately resulting in pleiotropic defects [16,17]. * Co...

Ngày tải lên: 12/08/2014, 03:21

... protein preparation and Western blot analysis was performed fol- lowing the methods described previously. Equal amounts of protein were prepared in 50 μlof Laemmli loading buffer andresolvedusing4-15% Figure ... warranted. In summary, we have identifi ed novel activating EGFR mutations in MPM associated with optimal resectability and prolonged survival. Clinically these mutations...

Ngày tải lên: 09/08/2014, 03:22

... ampi- cillin. Twelve colonies were picked and grown in Luria Bertani media in the presence of ampicillin. Plasmid extraction was performed by the alkaline lysis method and purified for sequencing. Results TP53 ... reported as involved in several other alterations, including deletions and insertions of one or several nucleotides. In 1994, Birch et al [3] reported a complex muta...

Ngày tải lên: 09/08/2014, 04:21

... sublexical information in Chinese. The games aim at implicitly informing players of the Chinese GPC rules, mimicking the process of how infants would apply statistical learning (Saffran et al., ... read- ing ability, e.g., (Siok and Fletcher, 2001). Moreo- ver, the ability to detect and apply phonological consistency in GPCs, including the roles of PCs in PSCs in Chinese, pl...

Ngày tải lên: 19/02/2014, 19:20

... time- consuming and costly task. In this paper, we pro- pose a framework for automatically identifying reasons in online reviews and introduce a novel technique to automatically label training data ... them and Section 4 explains our automatic data labeling process. Section 5 describes experimental and results and finally, in Section 6, we conclude with future work. 2...

Ngày tải lên: 20/02/2014, 12:20

... coefficients (http://www.expasy.org). Immunoblotting and in vitro kinase activity assay Proteins purified from BL21(DE3) cells were subjected to SDS ⁄ PAGE and Western blotting. Antibodies including anti-Src IgG (Millipore, Billerica, ... clamp recordings in HEK-293 cells have been described previously [17,29]. In brief, cells were bathed in a standard extracellular solution contain...

Ngày tải lên: 06/03/2014, 01:20

... (a) Class I includes polyhydric alcohols (sorbitol, glycerol, xylitol, adonitol, mannitol) and amino acids and derivatives (glycine, alanine, proline, serine, lysine, b-alanine and taurine) that ... d-stachyose, glycine, l- alanine, l- proline, l- lysine, l- serine, b-alanine, taurine, sarcosine, dimethylglycine, glycine betaine, trimethylamine N-oxide, and cytidine 2¢-3¢ cyclic m...

Ngày tải lên: 07/03/2014, 00:20

... the origins of selectivity and specificity. In all crystal structures solved to date, the ligand-binding position, as well as the positioning of the residues lining the binding site, is extremely well ... are replaced by Leu and Ile, water molecules are attached to the serines in all other TetR structures and fill a cleft between the ligand and the protein. Close inspec- tion of the...

Ngày tải lên: 16/03/2014, 00:20

... catalase (Cat -L) , enolase (Eno1 -L) , alanine-glyoxylate aminotransferase (Agt -L) , actin (Actin -L) and glyoxylate reductase ⁄ hydroxypyruvate reductase (Grhpr -L) from liver. (B) Western blot analyses of enolase ... capillar- ies were equilibrated for 15 min in reducing buffer contain- ing 50 mm Tris ⁄ HCl, pH 8.8, 30% glycerol, 6 m urea, 2% SDS and 1% dithiothreitol, followed by a...

Ngày tải lên: 23/03/2014, 03:20

... containing 500 mm NaCl plus 1 lL protease inhibitor cocktail (Calbiochem) per 20 mg cellular pellet. After 10 cycles of 2 min vortexing and 1 min resting on ice, the cell lysate was centrifuged ... stimulated by car- diolipin (Fig. 6A, lanes 3 and 2, respectively, and Fig. 6B). In parallel, deglutathionylation by Grx2 (evaluated by anti-GSH blotting analysis) in the pres- ence of ca...

Ngày tải lên: 23/03/2014, 07:20