báo cáo khoa học: " Simultaneous mutation detection of three homoeologous genes in wheat by High Resolution Melting analysis and Mutation Surveyor®" pot

... detection of three homoeologous genes in wheat by High Resolution Melting analysis and Mutation Surveyor ® Chongmei Dong* 1 , Kate Vincent 1,2 and Peter Sharp 1 Address: 1 Plant Breeding Institute, ... analysis of mixed PCR amplicons containing three homoeologous gene fragments and sequence analysis using Mutation Surveyor ® software, aimed at s...

Ngày tải lên: 12/08/2014, 03:21

... blot of MCF10A cells after RNAi of Ku70. Protein expression levels of the Ku70 and the Ku80 protein are shown in both the LVTHM (control cell line) and Ku70i (RNAi of Ku70) cell line. Actin was ... 5:30 http://www.ro-journal.com/content/5/1/30 Page 2 of 7 are associated with the type of DSBs induced by radia- tions of differing LET and the mechanisms involved in t...

Ngày tải lên: 09/08/2014, 08:23

... methylation of CpG islands (CpGIs) in pro- moter regions is known to be involved in the silencing of tumor-suppressor genes, steroid receptors, cell adhesion molecules and cell cycle regulator genes and ... methylation of cancer- related genes in giant breast fibroadenoma: a case report. Journal of Medical Case Reports 2011 5:516. Figure 2 Immunostaining of ERa protei...

Ngày tải lên: 10/08/2014, 23:20

... obtained by Elliphant using the training data randomly ordered. The performance reaches its plateau using 90% of the training instances. Using different ordering of the training set we obtain ... variety of subjects occurring in the training data. In addition, reach- ing a precision of 92.0% for explicit subjects us- ing just 20% of the training data is far more ex- pensive i...

Ngày tải lên: 31/03/2014, 21:20

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Ngày tải lên: 07/08/2014, 20:23

... spectroscopy and DCE imaging are often unable to discriminate cancer from inflammation of the prostate. Therefore, intra-prostatic lesions as defined by MR should not be targeted for simultaneous integrated ... obtained from the MR Spectroscopy showing an elevated ratio of choline to citrate (depicted in red) in the right mid gland of the prostate. 3b: Area of the right m...

Ngày tải lên: 09/08/2014, 10:21

... following integrin activation involves a series of complex signaling events beginning with integrin activation and orchestrated activation of Rho GTPases [40], our results suggest that treatment of C 60 ... 231 cells were simultaneously stained with calcein and ethidium using a live-dead assay kit. Lack of red-colored cells and the presence of cells stained in green indicat...

Ngày tải lên: 11/08/2014, 00:22

... again for 5 minutes in a solution of PAH, followed by rinsing, and then for 5 minutes in a solution of ALP followed by rinsing. This step-wise multilayer assembly mediated by electrostatic interaction ... Amin F, Riedinger A, Parak WJ: In vitro and intracellular sensing by using the photoluminescence of quantum dots. Analytical And Bioanalytical Chemistry 2010, 397:9...

Ngày tải lên: 11/08/2014, 08:20

... virus by immunofluorescence in the intes- tine and cecal tonsils of chickens experimentally infected with M41 of IBV, Lucio & Fabricant [23] found that M41 can infect a variety of tissues and ... (CEK). The sensitivity of IHC in detecting IBV antigens in the CAM of inoculated eggs matched the virus reisolation and detection in CEK. Using IHC, antigens of IBV...

Ngày tải lên: 12/08/2014, 04:21

... G, Pugin J, Ricou B, Pittet D: Evaluation of rapid screening and pre-emptive contact isolation for detecting and controlling methicillin-resistant Staphylococcus aureus in criti- Page 3 of 3 (page ... MRSA carrier screening to inform patient isolation and decontamination procedures, and thereby to more effectively control cross- infection [4]. Several studies have indeed indicat...

Ngày tải lên: 12/08/2014, 23:22