... (2002) An engineered Escherichi a coli tyrosyl-tRNA synthetase fo r site-specific incorporation of an unnatural amino acid into proteins in eukaryotic translation and its application in a wheat ... protein-dye binding. Anal. B iochem. 72, 248–254.24. Pratt, J.M. (1984) Coupled transcription-translation in prokary-otic cell-free systems. In Transcription and Translation(Hames, B.D. & Higgins, ... [15N]glycine,L-[15N]histidine [aN] .HCl,L-[15N]methionine,L-[15N]isoleucine,L-[15N]phenyl-alanine,L-[15N]serine,L-[U-15N]tryptophan,L-[15N]tyrosineandL-[15N]valine were purchased from Cambridge I sotopeLaboratories (Andover, MA, USA).L-[15N]Alanine,L-[15N]leucine...