báo cáo khoa học: " Recovery and characterization of a Citrus clementina Hort ex Tan ''''''''Clemenules'''''''' haploid plant selected to establish the reference whole Citrus genome sequence" pot

báo cáo khoa học: " Recovery and characterization of a Citrus clementina Hort. ex Tan. ''''Clemenules'''' haploid plant selected to establish the reference whole Citrus genome sequence" pot

... 2.3 and 4.6 cm. The max-imum value of the haploid plants was similar to the leaflength of the aneuploid plant (4.4 cm).Histological characterization The histological structure of anthers of the ... BiologyOpen AccessResearch article Recovery and characterization of a Citrus clementina Hort. ex Tan. 'Clemenules' haploid plant selected to establish the reference whole Citrus genome ... (from haploid to double haploid) increased the area, length and width of leaves. The floralorgans of haploid clementine were smaller than those of the diploid parental plant. The haploid ovaries,...

Tài liệu Báo cáo khoa học: Production and characterization of a secreted, C-terminally processed tyrosinase from the ﬁlamentous fungus Trichoderma reesei ppt

... ATT CCA TCA TCA TCATCA TCA TCA GGG CAC GAC ACA CAT CCC C; and reverse primer, GAT CGG TAC CTC ATT ACA GAGGAG GGA TAT GGG GAA C. The PCR reaction wasdone as described above. The ampliﬁed PCR ... trypsin and the potential glycopeptides werebound to a ConA column. MALDI-TOF MS analysis of the eluted material revealed a few peptides, of which the largest had a molecular mass of 3255.309 Da. ... was ampliﬁed from genomic T. reesei DNAwith the following primers: forward, GGG GAC AAGTTT GTA CAA AAA AGC AGG CTA TCA TGC TGT TGTCAG GTC CCT CTC G; and reverse, GGG GAC CACTTT GTA CAA GAA...

Báo cáo khoa học: Synthesis and characterization of a new and radiolabeled high-afﬁnity substrate for H+/peptide cotransporters pdf

... was subtracted from the total uptake to calculate the carrier-mediated uptake. Inset:Eadie–Hofstee transformation of the speciﬁc Gly-Sar uptake data[S, Gly-Sar concentration (mM); v, uptake ... incubation periods from 10 min up to 8 h. The amount of Bip-Pro in the extracellular uptake mediumwas quantiﬁed according to the laboratory standard HPLC(La-ChromÒ; Merck-Hitachi, Darmstadt, ... SKPT cellsAfter characterization of Bip-Pro as a very high-afﬁn-ity and enzymatically stable substrate of PEPT1 and PEPT2, the compound was synthesized in radiolabeledform according to Fig. 1C...

Báo cáo khoa học: Discovery and characterization of a Coenzyme A disulﬁde reductase from Pyrococcus horikoshii Implications for the disulﬁde metabolism of anaerobic hyperthermophiles doc

... the P. horikoshii CoADR), and TG104 (5¢-CGCGCCATGGAAAAGAAAAAGGTAGTCATAA-3¢) and TG105 (5¢-CGCGGTCGACCTAGAACTTCAAAACCCTGGC-3¢) for the P. furiosus CoADR. The N terminus of the CoADRs were based ... lL. Oxidation of NAD(P)H was monitored at 340 nm. The standard oxid-ase assay used to monitor the puriﬁcation of phCoADRwas conducted with 100 lm NADH and FAD as above. A unit of CoADR activity ... act as an NADH oxidase in vivo, instead act-ing as a CoADR. This is only the second demonstra-ted CoA reductase activity, and the ﬁrst appearance of this activity in both the Archaea and in a...

Báo cáo khoa học: Production and characterization of a thermostable L-threonine dehydrogenase from the hyperthermophilic archaeon Pyrococcus furiosus docx

... was PCR ampliﬁed from chromosomal DNA of P. furiosus using the primers BG1279 (5¢-GCGCGCCATGGCATCCGAGAAGATGGTTGCTATCA, sense) and BG1297 (5¢-GCGCGGGATCCTCATTTAAGCATGAAAACAACTTTGCC, antisense), ... the initial activity of Pf-TDH was checked using butane-2,3-diol as substrate in the standard oxidation reaction and acetoin in the reduction reaction. The activity of Pf-TDH was signiﬁcantly increased ... spontaneously decarb-oxylates to aminoacetone and CO2, or is cleaved in a CoA-dependent reaction by 2-amino-3-ketobutyratecoenzyme A lyase to glycine and acetyl-CoA. Amino-acetone can be further converted...

Báo cáo khoa học: Isolation and characterization of a D-cysteine desulfhydrase protein from Arabidopsis thaliana pptx

... pots, and all plant tissue above ground wasused for the analyses. (A) Total RNA was extracted and 20 lg RNAwas loaded in each lane and blotted as indicated in Experimentalprocedures. To prove ... Nagasawa T, Ohkishi H, Kawakami B, Yamano H,Hosono H, Tani Y & Yamada H (1982) 3-chloro-d-ala-nine chloride-lyase (deaminating) of Pseudomonas putidaCR 1–1. Puriﬁcation and characterization ... from Arabidopsis and Brassica napus leaves. The inhibitors AOA and hydroxylamine were used in a concentration range of 50 lm to 50 mm. The d-CDes activity was reduced byAOA to about 45% and by...

Báo cáo khoa học: Production and characterization of a noncytotoxic deletion variant of the Aspergillus fumigatus allergen Aspf1 displaying reduced IgE binding ppt

... (5¢-GTCGTCTTGCGGTCACCTGGACATGCATCAACGAACAG-3¢) and Ct-Aspf1 (5¢-GTCGTCTTGGATCCTCTCGAGTCTCAATGAGAACACAGTCTCAAGTC-3¢). These primers contained BstEII and BamHI sites and were used to generate a fragment that wascloned in the same ... proteins as inhibitors: Aspf1, Aspf1D(7–22), a- sarcin and a- sarcin D(7–22). The inhibitor amount is plotted inlogarithmic scale. The standard deviation of the measurements isshown.Variants of the ... FEBSProduction and characterization of a noncytotoxicdeletion variant of the Aspergillus fumigatus allergenAspf1 displaying reduced IgE bindingLucı´ a Garcia´-Ortega1, Javier Lacadena1, Mayte...

Báo cáo khoa học: Detection and characterization of a novel extracellular fungal enzyme that catalyzes the speciﬁc and hydrolytic cleavage of lignin guaiacylglycerol b-aryl ether linkages pdf

... Ikeda1, Shinya Kajita1, Masaya Nakamura2 and Yoshihiro Katayama11Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology, Koganei, Tokyo,Japan;2Forestry ... large excess of water was added and the resultantprecipitate was collected as DHP-GOU.Isolation of the fungi and enzymeActivity of the b-aryl ether cleavage enzyme was assayed asdescribed in a ... conditions. Analysis of the products of the reactionrevealed the presence of guaiacylglycerol (GG) and 4MU(data not shown).Localization of enzymatic activity To conﬁrm the localization of the b-aryl...

Báo cáo khoa hoc:" Construction and characterization of a bovine BAC library with four genome-equivalent coverage" pptx

... characterization of a bovine BAC library with four genome- equivalent coverageAndré EGGEN a, ∗, Mathieu GAUTIER a ,Alain BILLAUTd, Élisabeth PETIT a , Hélène HAYES a ,Pascal LAURENT a , Catherine ... orso BAC clones analysed by FISH was chimeric. This BAC library increases the international genome coverage for cattle to around 28 genome equivalents and extends the coverage of the ruminant genomes ... contributes to increas-ing the genome coverage for cattle. Added to the already existing BAC libraries of 2.7 [1], 6 [2], 10 [11] and 5 [13] genome equivalents, the total coverage of the bovine genome...

báo cáo khoa học: " Cloning and characterization of a glucosyltransferase from Crocus sativus stigmas involved in flavonoid glucosylation" pdf

... complexes in the phe-nylpropanoid and flavonoid pathways. Physiol Plant 1999,107:142-149.59. Nakatsuka T, Sato K, Takahashi H, Yamamura S, Nishihara M: Clon-ing and characterization of the ... before anthesis (-2da), anthesis (da), one day after anthesis (+1da), and three days after anthesis (+3da), and in closed and open stamen (stc and sto), corm, tepals (pt) and style. Equal amounts ... differentialaccumulation of the mRNA in the tissues analysed. First-strand cDNAs were synthesized by RT from 2 μg of totalRNA using a first-strand cDNA synthesis kit (Pharmacia) and random primers....

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