báo cáo khoa học: " Cloning and characterization of a glucosyltransferase from Crocus sativus stigmas involved in flavonoid glucosylation" pdf

... (5'-AGCTGTCGATAAGATGGATATC-3'), and CsGT45-f2 (5'-GAGTGTCTTATCGCATCCT-3') as forward primers, and CsGT45-r1 (5'-GAAGCCAAGCTCTCCATCGTCGA-3') and CsGT45-r2 (5'-CTCCAGCTGGCCGAATGTGTTC-3')as ... CentralPage 1 of 16(page number not for citation purposes)BMC Plant BiologyOpen AccessResearch article Cloning and characterization of a glucosyltransferase from Crocus sativus stigmas involved ... complexes in the phe-nylpropanoid and flavonoid pathways. Physiol Plant 1999,107:142-149.59. Nakatsuka T, Sato K, Takahashi H, Yamamura S, Nishihara M: Clon-ing and characterization of the UDP-glucose:anthocyanin...

Báo cáo khoa học: " Isolation and characterization of Streptococcus sp.from diseased flounder (Paralichthys olivaceus )in Jeju Island" ppt

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Tài liệu Báo cáo khoa học: Cloning and characterization of CBL-CIPK signalling components from a legume (Pisum sativum) ppt

... C)AATTTC-3¢ PsCBL (degenerate forward)45¢-GTATCAGCTTC(C ⁄ T)TCAAATGTC-3¢ PsCBL (degenerate reverse)55¢-CCATCACAAGAAACTAGAGAAAC-3 PsCIPK (5¢UTR forward)65¢-TTAAGTACTATAAAT-ACACAGCCTA-3¢ PsCIPK (3¢UTR ... T)GC(C ⁄ G ⁄ T)AAGGT-3¢ PsCIPK (degenerate forward)25¢-ACAAA (A ⁄ C )A( A ⁄ G) (A ⁄ G ⁄ T )A( C ⁄ T) (A ⁄ C ⁄ G)ACACCACAAGACC)3¢ PsCIPK (degenerate reverse)35¢-CTTAT(C ⁄ G)AACAAGGAA (A ⁄ C)AATTTC-3¢ PsCBL ... AAR01663) and AtCBL3(AAM91280). The calcium binding domains (EF1–4) and calcineurin A binding domain are shown in the box. The dot in the EF1 box repre-sents the modiﬁed amino acids alanine (A) as...

Tài liệu Báo cáo khóa học: Cloning and characterization of two distinct isoforms of rainbow trout heat shock factor 1 ppt

... was a partial cDNA containing an insert of 983 nucleotidesÓ FEBS 2004 Rainbow trout HSF1 (Eur. J. Biochem. 271) 705 Cloning and characterization of two distinct isoforms of rainbowtrout heat ... PCR was performed with the M13forward primer (5¢-CCCAGTCACGACGTTGTAAAACG-3¢)asasenseprimerandHSF1-speciﬁc primers (forHSF 1a, 5¢-GAAGCAGCTTGTCCAGTACACTAA-3¢;forHSF1b,5¢-GAAGCAGCTGGTCCAGTACACCTC-3¢)asantisense ... DNA-binding domain of HSF, whereas cloneC2 contained an insert of 2771 nucleotides including introns and an ORF encoding 513 amino acids.By using 5¢ -and3 ¢-RACE, the full-length cDNAs of clones C1 and...

Báo cáo khoa học: Cloning and characterization of the genes encoding toxic lectins in mistletoe (Viscum album L) pot

... (UTR)obtained by RACE. The sequences of the primers were:5¢AAAATCTAGAGAAGCAAGGAACAATGAATG-3¢(5¢UTR) containing the XbaI recognition site for cloning, 5¢-AAAAATGCATGAAGTTGATTGCTTGCATTAACTCAT-3¢ ... ML3p A- chain (Fig. 6). Binding of therecombinant MLp A- chains with TA7 was detected in allsamples. Interaction of the native A- chains with TA7 and MNA4 in comparison with recombinant A- chains ... recombinant ML1p and ML2p A- chains could beseen as a single band  30 kDa. The recombinant ML3p and ML3.1p A- chain preparations always gave an addi-tional band of  60 kDa (which may be seen on...

Tài liệu Báo cáo khoa học: Production and characterization of a secreted, C-terminally processed tyrosinase from the ﬁlamentous fungus Trichoderma reesei ppt

... GTT GGA ATT CCA TCA TCA TCATCA TCA TCA GGG CAC GAC ACA CAT CCC C; and reverse primer, GAT CGG TAC CTC ATT ACA GAGGAG GGA TAT GGG GAA C. The PCR reaction wasdone as described above. The ampliﬁed ... tyrosinase was alsoinactivated completely within 20 min [40]. In general,mammalian and plant-derived tyrosinases are not verythermostable; even a short incubation at 70–90 °C inac-tivates ... sig-niﬁcantly lower molecular mass (43.2 kDa) than that calculated from theputative amino acid sequence (61.151 kDa). According to N-terminal and C-terminal structural analyses by fragmentation,...

Báo cáo khoa học: Synthesis and characterization of a new and radiolabeled high-afﬁnity substrate for H+/peptide cotransporters pdf

... sub-strates of H+⁄ peptide cotransporters, such as Gly-Sar,Ala-Ala, Lys-Lys, Ala-Asp, d-Phe-Ala, Ala-Ala-Ala,d-aminolevulinic acid, cefadroxil and Ala-4-nitroanilide(all 100 lm, Table 1). Glycine, ... JC, Fujita T, Liang R, Ganapathy V & Lei-bach FH (1998) Identiﬁcation of a potential substratebinding domain in the mammalian peptide transportersPEPT1 and PEPT2 using PEPT1-PEPT2 and PEPT2-PEPT1 ... containing aromatic amino acids(24–34% trans content) obtained in a previous study of our group [23].To determine the stability of Bip-Pro, buffer sampleswere analyzed after incubating Caco-2...

Báo cáo khoa học: Discovery and characterization of a Coenzyme A disulﬁde reductase from Pyrococcus horikoshii Implications for the disulﬁde metabolism of anaerobic hyperthermophiles doc

... (5¢-GGCCTCATGAAGAAAAAGGTCGTCATAATT-3¢), and TG101 (5¢-GGCCAAGCTTCTAGAACTTGAGAACCCTAGC-3¢) (for the P. horikoshii CoADR), and TG104 (5¢-CGCGCCATGGAAAAGAAAAAGGTAGTCATAA-3¢) and TG105 (5¢-CGCGGTCGACCTAGAACTTCAAAACCCTGGC-3¢) ... isoalloxa-zine ring resulting in a loss of absorbance at thesewavelengths. During the addition of the ﬁrst equivalent of NADPH there is a decrease in absorbance at460 nm with a concomitant increase ... theratio of protein concentration to ﬂavin concentration(as determined at 460 nm). blast and tfasta analysis of the phCoADR revealed a signiﬁcant level of identityto putative NADH oxidases from...

Báo cáo khoa học: Production and characterization of a thermostable L-threonine dehydrogenase from the hyperthermophilic archaeon Pyrococcus furiosus docx

... (5¢-GCGCGGGATCCTCATTTAAGCATGAAAACAACTTTGCC, antisense), containing NcoI and BamHI sites (underlined in the sequences). In order tointroduce an NcoI restriction site, an extra alanine codon(GCA) was introduced ... short-chain AdhA and an iron-containing AdhB encoded bythe lamA operon [10], and an oxygen-sensitive, iron and zinc-containing alcohol dehydrogenase which hasbeen puriﬁed from cell extracts of P. ... preculture was used toinoculate 1 L Luria–Bertani medium with kanamycin and spectinomycin (both 50 mgÆL)1) in a 2-L conical ﬂask and incubated in a rotary shaker at 37 °C until a cell density of A 600¼...

Báo cáo khoa học: Cloning and expression of a tomato cDNA encoding a methyl jasmonate cleaving esterase pdf

... application of JA. These experiments demonstrate that individual mem-bers of the jasmonate family are involved – at least in Arabidopsis – in different signalling pathways.An Arabidopsis(jar1)mutantwithadefectinthejasmonate ... t heyare capable of mediating a response by regulating geneexpression [6,7]. Analysis of Arabidopsis thaliana mutantsimpaired in either JA biosynthe sis or signalling, gave a deeper insight ... aremediated by MeJA through JA, indicating that MeJA is notamediatoronitsowninthisparticularsystem.On the other hand, OPDA and JA can induce identicalgenes a s w ell as d istinct s ets o f t arget...

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