báo cáo khoa học: " Cloning and characterization of a glucosyltransferase from Crocus sativus stigmas involved in flavonoid glucosylation" pdf

... (5'- AGCTGTCGATAAGATGGATATC-3'), and CsGT45-f2 (5'- GAGTGTCTTATCGCATCCT-3') as forward primers, and CsGT45-r1 (5'-GAAGCCAAGCTCTCCATCGTCGA-3') and CsGT45-r2 (5'-CTCCAGCTGGCCGAATGTGTTC-3') as ... Central Page 1 of 16 (page number not for citation purposes) BMC Plant Biology Open Access Research article Cloning and characterization of a...

Ngày tải lên: 12/08/2014, 03:21

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Ngày tải lên: 07/08/2014, 18:21

... C)AATTTC-3¢ PsCBL (degenerate forward) 45¢-GTATCAGCTTC(C ⁄ T)TCAAATGTC-3¢ PsCBL (degenerate reverse) 55¢-CCATCACAAGAAACTAGAGAAAC-3 PsCIPK (5¢UTR forward) 65¢-TTAAGTACTATAAAT-ACACAGCCTA-3¢ PsCIPK (3¢UTR ... T)GC(C ⁄ G ⁄ T)AAGGT-3¢ PsCIPK (degenerate forward) 25¢-ACAAA (A ⁄ C )A( A ⁄ G) (A ⁄ G ⁄ T )A( C ⁄ T) (A ⁄ C ⁄ G)ACACCACAAGACC)3¢ PsCIPK (degenerate reverse) 35¢-CTTAT(C ⁄ G)AACAAGGAA (A...

Ngày tải lên: 19/02/2014, 07:20

... was a partial cDNA containing an insert of 983 nucleotides Ó FEBS 2004 Rainbow trout HSF1 (Eur. J. Biochem. 271) 705 Cloning and characterization of two distinct isoforms of rainbow trout heat ... PCR was performed with the M13 forward primer (5¢-CCCAGTCACGACGTTGTAAAA CG-3¢)asasenseprimerandHSF1-specific primers (for HSF 1a, 5¢-GAAGCAGCTTGTCCAGTACACTAA-3¢;for HSF1b,5¢-GAAGCAGCTGG...

Ngày tải lên: 19/02/2014, 12:20

... (UTR) obtained by RACE. The sequences of the primers were: 5¢AAAATCTAGAGAAGCAAGGAACAATGAATG-3¢ (5¢UTR) containing the XbaI recognition site for cloning, 5¢-AAAAATGCATGAAGTTGATTGCTTGCATTAAC TCAT-3¢ ... ML3p A- chain (Fig. 6). Binding of the recombinant MLp A- chains with TA7 was detected in all samples. Interaction of the native A- chains with TA7 and MNA4 in comparison with r...

Ngày tải lên: 07/03/2014, 15:20

... GTT GGA ATT CCA TCA TCA TCA TCA TCA TCA GGG CAC GAC ACA CAT CCC C; and reverse primer, GAT CGG TAC CTC ATT ACA GAG GAG GGA TAT GGG GAA C. The PCR reaction was done as described above. The amplified ... tyrosinase was also inactivated completely within 20 min [40]. In general, mammalian and plant-derived tyrosinases are not very thermostable; even a short incubation at 70–90 °C inac- ti...

Ngày tải lên: 19/02/2014, 06:20

... sub- strates of H + ⁄ peptide cotransporters, such as Gly-Sar, Ala-Ala, Lys-Lys, Ala-Asp, d-Phe-Ala, Ala-Ala-Ala, d-aminolevulinic acid, cefadroxil and Ala-4-nitroanilide (all 100 lm, Table 1). Glycine, ... JC, Fujita T, Liang R, Ganapathy V & Lei- bach FH (1998) Identification of a potential substrate binding domain in the mammalian peptide transporters PEPT1 and PEPT2 using P...

Ngày tải lên: 07/03/2014, 05:20

... (5¢-GGCCTCATGAAGAAAAAGGTCGTCA TAATT-3¢), and TG101 (5¢-GGCCAAGCTTCTAGAAC TTGAGAACCCTAGC-3¢) (for the P. horikoshii CoADR), and TG104 (5¢-CGCGCCATGGAAAAGAAAAAGGTA GTCATAA-3¢) and TG105 (5¢-CGCGGTCGACCTAGAA CTTCAAAACCCTGGC-3¢) ... isoalloxa- zine ring resulting in a loss of absorbance at these wavelengths. During the addition of the first equivalent of NADPH there is a decrease...

Ngày tải lên: 07/03/2014, 17:20

... (5¢-GCGCG GGATCCTCATTTAAGCAT GAAAACAACTTTGCC, antisense), containing NcoI and BamHI sites (underlined in the sequences). In order to introduce an NcoI restriction site, an extra alanine codon (GCA) was introduced ... short- chain AdhA and an iron-containing AdhB encoded by the lamA operon [10], and an oxygen-sensitive, iron and zinc-containing alcohol dehydrogenase which has been...

Ngày tải lên: 16/03/2014, 14:20

... application of JA. These experiments demonstrate that individual mem- bers of the jasmonate family are involved – at least in Arabidopsis – in different signalling pathways. An Arabidopsis(jar1)mutantwithadefectinthe jasmonate ... t hey are capable of mediating a response by regulating gene expression [6,7]. Analysis of Arabidopsis thaliana mutants impaired in either JA bios...

Ngày tải lên: 16/03/2014, 18:20