... Cu2+[(iii) and (iv)], Zn2+[(i) and (ii)] or EDTA [(v) and (vi)] and continued for 164 min. After 100 min, 400 lM EDTA was added, and the absorbance wasmeasured for an additional 64 min. (B) ThT analysis ... Y, Tanemura K, Murayama O, Akagi T,Murayama M, Sato S, Sun X, Tanaka N & Takashima A (2001) New insights on how metals disrupt amyloidbeta-aggregation and their effects on amyloid-betacytotoxicity. ... pitch of 0.5 nm, and a scan rate of 20 nmÆs)1, in a 2 mm quartz cuvette.AFM A portion of each Ab aggregate sample was diluted in water to approximately 1 lm peptide and applied to freshlycleaved...