báo cáo khoa học: " Selection of reference genes for quantitative real-time PCR expression studies in the apomictic and sexual grass Brachiaria brizantha" ppt

... BbrizUBCE and BbrizTUB were again the more stable and the least stable genes respectively, while there was a slight difference in the order of stability of the other genes. The second and third best reference ... both apomictic and sexual reproduc- tion within Brachiaria makes it an interesting system for understanding the molecular pathways involved in...

Ngày tải lên: 12/08/2014, 03:20

... was the same for both lines: using the proposed cut-off of 0.15 and comparing single developmental stages, the required number was two for Mitchell and V30. The requirement for only two genes is ... log-transformed and used as input for the NormFinder tool, which fitted this data into a Figure 3 Expression profiling of reference genes in different organs...

Ngày tải lên: 12/08/2014, 03:21

... the high number of drugs that ICU patients receive, the preference for intravenous administration and the incidence of organ failure, all of which increase the potential for errors [11,12]. Studies ... after the completion of the study, the ICIS was implemented in the two other remaining units. Approval of the ethics committee was obtained; informed conse...

Ngày tải lên: 25/10/2012, 10:39

... to protect against candidiasis, for the serodiagnosis of candidiasis, or for the identification of mechanisms of Candida infection. Furthermore, studies have indicated the b-1,2-linked mannose ... containing three b-1,2 linkages obtained from the mannan of C. albicans in a preceding study con- sisted of isomers. The molar ratio of the side chains of C. lusitan...

Ngày tải lên: 17/03/2014, 03:20

... responsible for binding and infection in E. coli.Thus, incorporation of a library of target proteins between the N-terminal domain and the C-terminal (CT) domain allows a protease-based selection ... different thermodynamic stability were inserted into the region between the C-terminal region and the two N-terminal domains of the g3p. After several rounds of pr...

Ngày tải lên: 19/02/2014, 12:20

... pMAZ360– IgG expression vector contains the packaging signal of f1 bacteriophage that enables the packaging of the plasmid as ssDNA in the presence of a helper phage, Selection of IgG by tandem phage ... displays the Fc-binding ZZ protein [45] on all five copies of the gene-3 minor coat protein, but maintains its ability to infect and propa- gate in E. coli ⁄ F¢...

Ngày tải lên: 22/03/2014, 21:21

... performed on the region spanning the HCDR3 sequence including Cys92 and Cys104 (Fig. 3B). The replacement of Cys92 and Cys104 resulted in 60% and 90% decreases in binding, respectively. In addition, ... direct link between the DNA information and the peptide displayed at the surface of the phage. This technology has become a standard approach for the identi...

Ngày tải lên: 28/03/2014, 22:20

... 59% of the considered residues). Trp is present in 19% of these three residues, Leu in 11% and Met and Phe in 7%. Second, the Pro is present in 18.5% of the peptide sequences. Binding of the ... second round of negative selection in our experimental conditions. On the one hand, there is an increase in the yield of specific peptides recovered, but on t...

Ngày tải lên: 31/03/2014, 01:20

... the commercial animals will be the grandoffspring of the individuals considered and, therefore, the progeny of one of the 18 potential grandparental combinations, assuming ... with the standard method, and in the cases of complete dominance and overdominance, the avoidance of inbreeding is maximum. However, in the case of d = -1...

Ngày tải lên: 09/08/2014, 18:21

... genome-wide screening for parent -of- origin specific uniparental expression in seed tissues will facilitate our understanding of the origins and functions of imprinted genes in flowering plants. Results: ... imprinted candidate genes for further inves- tigation. We confirmed expression of the three candi- dates in 4 dap seeds by LCM RT -PCR and further confir...

Ngày tải lên: 11/08/2014, 11:21