báo cáo khoa học: " Characterization of two Arabidopsis thaliana acyltransferases with preference for lysophosphatidylethanolamine" ppt

... can- didate acyltransferases. However as one of the Arabidopsis enzymes turned out to have strong dual activity with high LPAAT activity, we also decided to express the Arabidopsis acyltransferases ... of microsomal protein added to the assays of the ale1 transformants were: for LPS, 1.88 μg, LPC and LPE, 1.33 μg, and LPA, 1.16 μg, and in the assay of LPA as an acceptor in...

Ngày tải lên: 12/08/2014, 03:20

... 2003 fraction of S. oryzae WAA42 was then used for further characterization, with 1–3 lg of protein loaded per assay, in order to work in the linear part of the curve. The proteinaceous origin of the ... °C and used within a month after labeling. For iodination of the peptide using nonlabeled iodine, the protocol was as described above, except that 200 lgofthe 3741 Da isoform...

Ngày tải lên: 08/03/2014, 02:21

... using 2 mL of 0.1 M sodium methoxide for 5 min at 90 °C. Preparation of methyl esters from the seeds were performed by treatment of whole seeds with 2 mL of 0.1 M sodium methoxide for 55 min ... transformed with the D6acetyl- enase gene. It was of particular interest to investigate the stereochem- istry in the removal of hydrogen atoms in the formation of the two geome...

Ngày tải lên: 16/03/2014, 18:20

... exon 2 of the DNASE1. Conditions for these amplifi- cations were 95 °C for 9 min, 30 cycles of 94 °C for 1 min, 60 °C for 1 min, and 72 °C for 2 min, followed by incuba- tion at 72 °C for 10 min. ... mixture with 6 lL of Lipofectamine Plus reagent at room temperature for 10 min. Four microliters of Lipofectamine Plus reagent were diluted in 100 lL of Opti-MEM I-reduce...

Ngày tải lên: 19/02/2014, 06:20

... interaction partner for the C-termi- nus of Arabidopsis thaliana plasma membrane H + -ATP- ase (AHA1 isoform): site and mechanism of action on H + -ATPase activity differ from those of 14-3-3 pro- teins. ... activation of Fig. 2. Stimulation of A. thaliana PM H + -ATPase activity as a func- tion of the concentration of His 6 PPI1 88 and PPI1 588 His 6 . PM treat- ment w...

Ngày tải lên: 19/02/2014, 07:20

... V max /K m - quotient was twofold higher for d- than for c-tocopherol. Kinetics of Arabidopsis TMT The following kinetic analysis was performed for the forward reaction in the presence and absence of inhibitors. Substrate ... here we performed a detailed biochemical characterization of a c-tocopherol methyltransferase (c-TMT) from Arabidopsis thaliana and of a c-TMT puri...

Ngày tải lên: 17/03/2014, 09:20

... l M ,therelative amplitude of the maintained current was always higher for hNa v 1.5 (10–19% of the peak) than for rNa v 1.4 (2–6% of the peak). The order of potency of toxins was the same for the two muscular ... the release of histidine upon digestion of the toxin with carboxypeptidase A. Comparison of primary structures of representative a-mammal, a-insect and a...

Ngày tải lên: 17/03/2014, 11:20

... Knowledge of the structure of mutated DNA will contribute to the effective exploitation of heavy-ion beam mutagenesis. Results: Dry Arabidopsis thaliana seeds were irradiated with carbon (C) ions with ... total volume of 10 µl with PCR-grade water. The reaction conditions comprised an activation step at 95°C for 10 min followed by 50 cycles of 95°C for 10 s, a tou...

Ngày tải lên: 11/08/2014, 11:21

... 12 Co-regulatory networks of Arabidopsis WRKY genes. Co-regulatory networks of Arabidopsis WRKY genes obtained with the PCC threshold value of 0.7 in the untransformed (P-lin) (panel A) and log transformed ... 4 Co-regulatory networks of Arabidopsis WRKY genesFigure 4 Co-regulatory networks of Arabidopsis WRKY genes. For each pair of WRKY genes, the Pearson Coefficie...

Ngày tải lên: 12/08/2014, 03:21

... deconvolution of spectra of the type shown in Fig. 1A¢, with ML signals of a very low intensity, was performed as a first step. Deconvolution of spectra of the type shown in Fig. 1A, with intense ML ... apoptosis of tumour cells with the level of reduced glutathione, as detected by 1 H NMR [14]. These cells were therefore considered for use in mag- netic resonance spectro...

Ngày tải lên: 18/02/2014, 13:20