báo cáo khoa học: " Utility of EST-derived SSR in cultivated peanut (Arachis hypogaea L ) and Arachis wild species" potx

... bud- burst will result in a greater height and diameter growth for families. Growth termination and leaf fall appear closely related in cloned full-sibs. Late growing will increase ... flower receptivity and pollination. Pollination usually took place in March. During receptivity and pollination, the grafted branches were isolated in individual...

Ngày tải lên: 09/08/2014, 02:21

... EST- SSR in 16 accessions of wild species was evaluated. The results showed that 221 of 251 EST -SSR loci (88 %) were polymorphic (Table 3), with a total of 867 alleles (Addi- tional File 1). Allelic ... in the Polyacrylamide gel electrophoresis patterns of microsatellite alleles amplified with the primer EM-31Figure 1 Polyacrylamide gel electrophoresis patterns of m...

Ngày tải lên: 12/08/2014, 03:20

... Lou L, Kohli RM, Onishi M, Linne U, Marahiel MA & Walsh CT (200 2) Timing of epimerization and con- densation reactions in nonrbosomal peptide assembly lines: kinetic analysis of phenylalanine ... originating from elongation modules. Interestingly, the ‘aminoacyl-’E domains seem to be tolerant towards C-terminal varia- tions of neutral hydrophilic amino acids of the pep- t...

Ngày tải lên: 30/03/2014, 20:20

... genic -SSR markers, including a range of allele sizes, number of alleles and PIC values among 30 genotypes Sr. No. Marker Id. Allele size (bp) Number of alleles PIC Value Cultivars Wild Total Cultivars ... cajan (L. ) Millspaugh] is an impor- tant food legume predominantly cultivated in the tropi- cal and subtropical regions of Asia and Africa. It is a diploid (2n = 2...

Ngày tải lên: 11/08/2014, 11:21

... 7. 4) containing protease and phosphatase inhibitor cocktail set (Calbiochem, La Jolla, CA, USA). Aliquots of 500 lg of protein diluted to 1 mgÆmL )1 in RIPA buffer were precleared with protein ... Guo T, Vallejo J & Karpen SJ (200 8) Regulation of hepatic drug-metabolizing enzyme genes by Toll-like receptor 4 signaling is independent of Toll-interleukin 1 receptor do...

Ngày tải lên: 14/02/2014, 18:20

... distribution of Nile red fluorescence intensity of individual cells at 3, 6, 12, 24 and 36 h were obtained by flow cytometric analysis in the FL1 channel (logarithmic scale). Control (Ctrl) cells (blue line), ... cell types, including hepatocytes, to long-chain saturated fatty acids (SFAs) led to increased expression of proin- flammatory cytokines, inhibition of insulin signaling, in...

Ngày tải lên: 14/02/2014, 22:20

... Okamura-Oho Y & Callahan J (199 3) The 67-kD elastin ⁄ laminin-binding protein is related to an enzymatically inactive, alterna- tively spliced form of beta-galactosidase. J Clin Invest 91, 1198–1205. 24 ... 88% of all Lys residues in tropoela- stin participating in covalent linkages that are crucial for the formation of mature elastin [78]. As elastin cross-links are not enz...

Ngày tải lên: 16/02/2014, 14:20

... domains: the extracellular ligand-binding domain, characterized by leucine-rich repeats and the intracellular Toll ⁄ IL-1 receptor domain (TIR domain) [3]. In mammals, 13 TLR homologues recognizing ... were also found in the murine macrophage cell line J774. The ligand for intracellular TLR9 modulates RGS1 and RGS2 in a similar manner. However, the TLR3 ligand poly(I:C) permanently...

Ngày tải lên: 18/02/2014, 13:20

... Co. Ltd., Tokyo, Japan Ceramide (Cer) has been implicated in various cellular processes including proliferation, apoptosis and cell signaling [1]. Intracellular Cer levels are strictly regu- lated ... a region of the protein that is known to be essential for kinase activity and contains a diacylglycerol kinase-like catalytic domain [2]. As shown in Fig. 5A, cell death and limited...

Ngày tải lên: 18/02/2014, 18:20

... (v/v) Nonidet P-40 allowing swelling and lysis of the cell membrane. The lysate was recentrifuged at 300 g at 4 °C and the resulting nuclear pellet was resuspended in 150 lL of buffer containing 20 ... these signaling molecules is prominent in the regulation of cell cycle and cell differentiation particularly in stress-related pathologies including hypertension, bladder o bs...

Ngày tải lên: 19/02/2014, 16:20