báo cáo khoa học: " Regulation of callose synthase activity in situ in alamethicin-permeabilized Arabidopsis and tobacco suspension cells" potx

... 1 of 13 (page number not for citation purposes) BMC Plant Biology Open Access Research article Regulation of callose synthase activity in situ in alamethicin-permeabilized Arabidopsis and tobacco ... effect of polymerized tubulin on callose synthase activity. No callose was deposited unless alamethicin, Ca 2+ and UDP-glucose were present. Tubulin and ca...

Ngày tải lên: 12/08/2014, 03:20

... 1.8 nm, depicting a strong interaction between 2A2 mAb and cathepsin B. Determination of the 2A2 mAb binding site on cathepsin B The binding site of 2A2 mAb on cathepsin B was determined by SPOT ... corresponding to cathepsin B ⁄ cystatin C complex and the appearance of a new peak at 11.28 mL. The western blot (insert) shows the absence of cystatin C and the presence of cathep...

Ngày tải lên: 18/02/2014, 11:20

... fixed, permeabilized and stained for F-actin with rhodamine-conju- gated phalloidin. Th e effects of toxin B and latrunculin B on the actin filaments was examined by preincubating the cells with either ... s pecifically and e ffectively induced maximal inhibition of Erk1/2, p38, JNK1/2 and PtdIns 3-kinase [18,30]. The incubation was further contin- ued in the presence of S1P, anis...

Ngày tải lên: 19/02/2014, 16:20

... processing of one or several nuclear proteins, whereas the other is independent of the processing capacity of the protein- ase and involves the HPX domain. A DNA-binding domain was found in the ... autocatalytic step of the activation by binding to the 64 kDa intermediate form of MMP-2 [52]. Binding and activation of MMP-2 was abrogated in the pres- ence of avb3 integrin...

Ngày tải lên: 15/03/2014, 00:20

... balance of the above activities. Comparing phosphatase and kinase inhibitors The protein phosphatase inhibitor OA and the protein kinase inhibitor, the broad spectrum inhibitor of pro- tein kinase ... release of cytochrome c from mitochon- dria into the cytosol through inactivation with Bcl-xL and Bcl-2 [69]. Inactivated Bax and Bad bind 14-3-3, the phosphoserine ⁄ threonine bin...

Ngày tải lên: 14/02/2014, 22:20

... generation of heterochromatin, and reduced the binding of HSF1. These results show that HSF4 promotes the DNA-binding activity of another mem- ber of the HSF family, through modulating the chro- matin ... [91–93]. Recently, Cullin3, a subunit of a Cullin-RING E3 ubiquitin ligase, was reported to interact with the enriched in proline, glutamate, serine and threonine (PEST) se...

Ngày tải lên: 18/02/2014, 04:20

... competitive inhibition of dCTP binding by dTTP. However, the presence of dTTP in the assay incubation also increased the apparent cooperativity of dCTP bind- ing, which indicates that the mechanism of ... designated A and B, originating from the A and B chains in the structure. dTTP binds at the site of the protein shown previously to bind the nucleotides dUTP and dCTP in...

Ngày tải lên: 18/02/2014, 16:20

... published on PEVK domain-containing titin frag- ments (see Introduction for references). The PEVK domain remains the main actin-binding candidate identified in the I-band titin region and we propose, ... entire length of thin filaments and is capable of modulating the r ate of formation of the a ctin– myosin complex [74,75]. Interestingly, nebulin also interacts with the titin PEV...

Ngày tải lên: 19/02/2014, 16:20

... infection. ArgII is involved in the biosynthesis of natural poly- amines (putrescine, spermidine and spermine) through conversion of l-arginine into l-ornithine [16]. The lat- ter is in turn used by ... Activation of p38 mitogen-activated protein kinase and c-Jun NH(2)-terminal kinase by double- stranded RNA and encephalomyocarditis virus: involve- ment of RNase L, protein k...

Ngày tải lên: 07/03/2014, 21:20

... proteins and involves mainly post-translational modification of pre- existing proteins [52]. The search for molecules regulating the activity of c-fos and c-jun revealed the existence of an intricate ... SRF of an, as yet, unidentified accessory factor [54]. The regulation of c-jun is complex and may involve an increase in the levels of c-jun protein and/ or phosphoryla...

Ngày tải lên: 08/03/2014, 08:20