Báo cáo y học: "Functional dissection of the alphavirus capsid protease: sequence requirements for activity" ppsx

... have analyzed for the first time the sequence requirements for the cleavage activity of ChikV capsid protease of genus alphavirus. We also show further evidence about the role of trypto- phan ... C-terminus to a nchor the tryp- tophan residue into the active site [4, 5]. Thus, we set out to analyze the influence of the C-terminal aa sequence for the a...

Ngày tải lên: 12/08/2014, 02:20

... on the analysis of the Ydc2 atomic structure we proposed a model for the interaction of Ydc2 with the junction. In this model two arms are engaged in the catalytic sites for cleavage while the ... It may be that precise interactions of the triple-helix domain with the arms of the junction are needed for the correct positioning of the catalytic sites for...

Ngày tải lên: 20/02/2014, 11:20

... the C-terminus of the receptor which is located on the inside of the cell, we then determined if signal transduction by the protein was affected by this modification. One of the consequences of ... version of CCR5 was present on the surface of transfected cells, and had the expected molecular mass. To test whether the presence of the myc-his tag at the C-ter...

Ngày tải lên: 08/03/2014, 09:20

... paper the complete resolution of the protein components of phycobilisomes from the cyanobacterium Synechocystis 6803, together with the determination of their molecular mass, has s uccessfully been ... is observed for the subcomplex contained i n the su crose band 2 where the amount of the b-phycocyanin component is significantly reduced. The allophycocyanins are ma...

Ngày tải lên: 08/03/2014, 16:20

... dependent upon the combined action of HNF1 and HNF3 as was reported for the human glucose transporter type 2 isoform gene [22]. Therefore the close distribution of these sites seems to play a different ... Each strand of the 5¢ flanking region of the Bsep gene was directly sequenced (Epidauros, Bernried, Germany) by automated sequencing. The sequence was analysed for...

Ngày tải lên: 17/03/2014, 23:20

... There is no current recommendation for the intervals between the scans [1]. Very close follow- Figure 3 Digital subtraction angiography of the superior mesenteric artery after the placement of ... metallic endoprosthesis. (A) The lateral arteriogram shows the final position of the endoprosthesis at the proximal part of the superior mesenteric artery (black arrows)...

Ngày tải lên: 11/08/2014, 12:20

... average of over all clusters by: The geometric accuracy (Acc) indicates the tradeoff between sensitivity and predictive value. It is obtained by computing the geometric mean of the Sn and the PPV by: Separation From ... algorithm always performs better than the other three. However, we found the size of predicted clusters might affect the number. The larger a cluster i...

Ngày tải lên: 14/08/2014, 08:20

... significantly by examining the genetic and physical context of the interactions. In other words, they attempt to place the transcriptional response to DNA damage in yeast within the context of the cell ... but efforts to do this are limited by tech- niques, by resource availability and perhaps by our concep- tualization of the nature of the biology. For instance, what...

Ngày tải lên: 14/08/2014, 17:22

... translation of the reporter gene. Alternatively the cells were left in the presence of the antibody for t he complete length of this period. In both cases the result was the same. As shown in Fig. 7, the ... the knowledge of some of the eff ects induced by tet raspanin proteins and the proteins with which they interact, the identification of the s ignallin...

Ngày tải lên: 08/03/2014, 22:20

... inhibition towards elastase and the lack of detectable inhibition of trypsin and chymotrypsin suggests that the physiolo- gical target of eppin’s antiprotease activity has yet to be discovered. By contrast, two ... 3). This is probably due to some of the cells in the control samples dying, thus partly masking the effects of the proteins. Interestingly, these killing effe...

Ngày tải lên: 23/03/2014, 07:20