Báo cáo y học: "A Functional Henipavirus Envelope Glycoprotein Pseudotyped Lentivirus Assay System" pdf

... the henipavirus G glycoprotein [15,46]. Thus, by a wide variety of well- known and well-characterized approaches the functional henipavirus envelope g lycoprotein pseudotyped lenti- virus assay ... this article as: Khetawat and Broder: A Functional Henipavirus Envelope Glycoprotein Pseudotyped Lentivirus Assay System. Virology Journal 2010 7:312. Submit your next m...

Ngày tải lên: 12/08/2014, 02:20

... incubated with gp130 antibody B-P8 (light gray histograms) or with LIFR antibody 10B2 (dark gray histograms) followed by phycoerythrin-conjugated secondary antibody. As a negative control, mock-transfected ... LIFR antibody 10B2 (LIFR) followed by phycoerythrin-conjugated secondary antibody (black histograms). As a negative control, mock-transfected cells were treated in the same way (gray hi...

Ngày tải lên: 18/03/2014, 01:20

... Zhang 1,2 *, Yoshifumi Muraki 1 , Taichi Hayashi 1 , Takanori Yasukochi 1,2 , Yuko Kori 1 , Daisuke Goto 1 , Satoshi Ito 1 , Akito Tsutsumi 1 and Takayuki Sumida 1 1 Clinical Immunology, University of ... [RF]), identified by screening, is commonly used as a diagnostic marker, although the patho- genic role played by autoantibodies in RA remains a mystery. Fcγ receptors (FcγRs) play a pivot...

Ngày tải lên: 09/08/2014, 07:20

... function/fewer symptoms) end of the scale and in the functional pain domain compared with functional difficulty. Discussion This study of the OA-FUNCTION-CAT functional difficulty and functional pain ... change among symptomatic patients at either end of the functional difficulty or functional pain Figure 2 Comparison of the item distributions for the functional difficulty and...

Ngày tải lên: 09/08/2014, 14:22

... bioluminescence Cytotoxicity Assay (Cell Technology Inc, Mountain View, CA), which detects secreted Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) in cells with diminished me mbrane integrity. Values ... Forty-eight hours after infection, a b-galactosidas e assay was used to measure infection in the cells as described above. Cell toxicity assays The toxicity of siRNA trea tment was meas...

Ngày tải lên: 13/08/2014, 01:20

... (K63W) (kindly provided by J. Ninomiya-Tsuji); pCMV-ΔTRAF2 (1- 211) (kindly provided by G. Natoli); and pcDNA3-HA-IKBKG (134-419) (kindly provided by G. Nuñez). High-throughput transfection and assay conditions High-throughput ... binding Nucleotide binding Catalytic activity Receptor activity Hydrolase activity Transferase activity Signal transducer activity Nucleic acid binding Receptor b...

Ngày tải lên: 14/08/2014, 07:21

... cause pulmonary parenchymal injuries because of the impossibility of determination of the pleural adhe- sions. Also pulmonary, cardiac, esophageal and main vas- cular injuries may occur by trochar ... funding of the study was provided by the authors. Author Details 1 Numune Teaching and Research Hospital, Division of Thoracic Surgery, Ankara, Turkey and 2 Dr. Nafiz Korez Sincan City Hospita...

Ngày tải lên: 10/08/2014, 10:20

... inflammatory mediators released by leukocytes in- duce a circulatory switch which favors the lymphovenous circulation. Lastly, the leukocytic phenotype is associated with enzymatic stress and hypermetabolism ... to systemic inflammatory response syndrome or SIRS, fol- lowed by a period of recovery mediated by counter-regu- latory anti-inflammatory response (CARS) [2,4,38,59,61]. Activated p...

Ngày tải lên: 13/08/2014, 23:21

... ADP-ribosy- lation activity. We show that the ExoS proteins interact with all isoforms of the 14-3-3 family. Finally, we show that the DALDL sequence is necessary for the ADP-ribosylation activity and ... to change the morphology of infected cells. Keywords: ADP-ribosylation; coenzyme binding site; cyto- toxicity; NAD-dependent; peptide inhibitor. Members of the 14-3-3 family function as adap...

Ngày tải lên: 31/03/2014, 08:20

... chondrocytes) shows superinduction of inflamma- tory mediators as observed by gene chip analysis, but fails to show the cardinal signs of inflammation [3]. These types of analyses will not only facilitate ... therapy and diagnosis. A system approach to arthritis ‘General System Theory’, proposed in 1940, has per- vaded all fields of science and has penetrated into popular thinking in psycholo...

Ngày tải lên: 09/08/2014, 01:21