... scintillant (Amersham). Each value is anaverage of four independent colonies and the assays wereperformed in triplicate.b-Galactosidase assayb-Galactosidase activity was assayed in cell extracts asdescribed ... T.S., Fukuma, M., Yano, K.I., Sakurai, H., Vonika, A. , Johnston, S .A. & Fukasawa, T. (1996) Analysis of thegalactose signal transduction pathway in Saccharomyces cerevi-siae: Interaction between ... that Gal4p activity iscontrolled by two independent signal transduction path-ways: a Gal3p–galactose pathway and the holoenzymeassociated cyclin dependent Srb10p kinase pathway. Theobservation...