Báo cáo y học: " Detection of swine transmissible gastroenteritis coronavirus using loop-mediated isothermal amplification" pptx

... Development and evaluation of a novel loop-mediated isothermal amplification method for rapid detection of severe acute respiratory syndrome coronavirus. Journal of Clinical microbiology 2004, 42:1956-1961. 10. ... in shrimps by loop-mediated isothermal amplification. Journal of Virological Methods 2006, 131:41-46. doi:10.1186/1743-422X-7-206 Cite this article as: Chen et a...

Ngày tải lên: 12/08/2014, 01:21

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Ngày tải lên: 07/08/2014, 20:23

... the sensitivity, specificity and accuracy (concordance) of MRI and radiography for detection of bone erosions in RA wrist bones. A second objective was to determine the intramodality and intermodality agreement ... showed moderate sensitivity and good specificity and accuracy for detection of erosions in rheumatoid arthritis and healthy wrist bones, while radiography showed very low...

Ngày tải lên: 09/08/2014, 10:23

... More reliable diag- nosis of infection with human immunodeficiency virus type 1 (HIV-1) by detection of antibody IgGs to pol and gag proteins of HIV-1 and p24 antigen of HIV-1 in urine, saliva, ... performed using 10 μl cDNA, forward and reverse primer pairs, dNTPs, Taq polymerase buffer and Taq-polymerase in thermocycler (Applied Biosystems) with cycling conditions of 94°C, 10...

Ngày tải lên: 10/08/2014, 05:21

... every day from 1 st day of hospitalisation. @1 - Day of admission. @2 - Day of discharge/death. Cecilia et al. Virology Journal 2011, 8:46 http://www.virologyj.com/content/8/1/46 Page 2 of 4 India/2009) ... suffered primary or secondary infections. HI antibody titre of >1:2560 in the acute phase of infection is considered confirmatory of secondary infection [8]. Case 1 had ve...

Ngày tải lên: 11/08/2014, 21:22

... waters. Typically we utilize monolayers of 3 cell lines; buffalo green monkey kidney (BGM), human colonic carcinoma (CAC O-2) and African rhesus monkey kidney (MA104) with the intent of provid- ing ... on day 6 at -80°C and stored until the RNA extraction proce dure was performed. Because of the highly lytic nature of poliovirus type 1 strain LSc-1 and the duration of the experiment...

Ngày tải lên: 12/08/2014, 01:22

... synthesized by Saituo * Correspondence: cuishangjin@yahoo.cn † Contributed equally 3 Division of Swine Infectious Disease, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary ... TheTaqManreal-timePCRassaywillbeusefulfor studying the molecular epidemiology of PPV infections in swine populations. The assay will also be very useful for early diagnosis of PPV and ther...

Ngày tải lên: 12/08/2014, 02:20

... AF013363, AY632535, AF013406, AY632540, AF013382, AF119661, DQ181799, AY702040 , U88535, DQ285561, AY708047, AF298807, AY762084, AY776330, AY762085, AY618988, AF100466, AY858044, AY858047 , AY744685, ... with ABI Prism BigDye Terminator Cycle sequencer v3.1 ready reaction (Applied Biosystems) and analyzed with the ABI PRISM 377 DNA Analyzer (Applied Biosystems). Sequence analysis was carrie...

Ngày tải lên: 12/08/2014, 04:21

... extraction by (a) alkaline lysis and (b) Chelex ® Results of PCR analysis of Chlamydia trachomatis EB in synovial samples following DNA extraction by (a) alkaline lysis and (b) Chelex ® . On the y- axes ... Schumacher HR Jr, Pando J: Comparison of synovial tissue and synovial fluid as the source of nucleic acids for detection of Chlamydia trachomatis by polymerase chain reaction...

Ngày tải lên: 12/08/2014, 11:22

... extension of both basal and apical emphy- sema. The results verify this hypothesis, and suggest that in future studies of potential emphysema-modifying ther- apy, targeted sampling may be of greater ... emphysema is frequently localised within characteristic regions of the lung [13], particularly in the early stages of disease. Con- sequently, densitometric assessment of the whole...

Ngày tải lên: 12/08/2014, 15:21