báo cáo khoa học:"Exclusion of known gene for enamel development in two Brazilian families with amelogenesis imperfecta" doc

... CentralPage 1 of 7(page number not for citation purposes)Head & Face MedicineOpen AccessResearchExclusion of known gene for enamel development in two Brazilian families with amelogenesis ... evaluated for linkage tochromosome regions known to contain genes important in enamel development. The presentstudy indicates that the AI in these two families is not caused by any of the known ... proteins in forming enamel are amel-ogenin, ameloblastin, and enamelin. These proteins areproteolytically cleaved following their secretion. Some of the cleavage products accumulate in the enamel...

Báo cáo khoa học: Role of conformational ﬂexibility for enzymatic activity in NADH oxidase from Thermus thermophilus pptx

... homologous ﬂavoproteinsform a novel ﬂavoprotein family in which all memberscontain an aromatic amino-acid residue (Phe, Trp) thatinteracts with the isoalloxazine ring of the ﬂavin cofactor[31]. ... containing Trp47would affect the interaction of the ﬂavin cofactor with Trp47 in the active site of the enzyme. This could be ageneral mechanism of substrate/enzyme interaction in thisﬂavoprotein ... the ﬂavin cofactor is possibleonly in the dimeric structure of NADH oxidase. None of thetryptophan residues is close to the binding site of the ﬂavincofactor in the monomeric form of the enzyme...

Báo cáo khoa học: "Choice of a model for height-growth in maritime pine curves" pps

... theinflexion point is p = mm/1-m, and there isno closed form solution for m in terms of p.This precludes the use of p for the CRmodel. Keeping m, the new form of the ... point isoften neglected in applications. In order to detect and avoid these poten-tial shortcomings, a preliminary investiga-tion was carried out and is presented in ... years, the zero pointwas included in the analysis. Two trees had non-sigmoidal curves.Nonlinear regression was made with a spe-cial software which use ordinary least-squaresestimation...

báo cáo khoa học: " Expressions of COX-2 and VEGF-C in gastric cancer: correlations with lymphangiogenesis and prognostic implications" ppsx

... peritumoral tissue of gastric carcinoma was highlighted byimmunostaining against D2-40 (LsAB,×200). B. Immunohistochemical staining of D2-40 in the intratumoral tissue of gastric carcinoma (LsAB,×200). ... brownish yellow in the cytoplasm of carcinoma cells (LsAB, ×400).Figure 2 Immunohistochemical staining of VEGF-C in t hegastric carcinoma: the positive expression of VEGF-C proteinwas stained as yellow ... was used. The procedure for immunohisto-chemical staining of D2-40 is similar to that of theCOX-2 staining at a dilution of 1:100.Evaluation of immunohistochemical stainingThe immunohistochemical...

báo cáo khoa học: " Identification of miRNAs and their target genes in developing soybean seeds by deep sequencing" doc

... 80% of these genesare involved in metabolic process, and reproduction-related genes were more enriched in miRNA targetsthan in soybean total genes. The enrichment of thegenes involved in metabolic ... detected because of incompleteinformation on gene sequences. However, miRNAsmainly cleave CDS of rice genes with relatively inte-grated gene sequences [13].Putative functions of annotated miRNA ... Reads overlapping with exons of protein-coding genes were excluded to avoid mRNA contamina-tion. The remaining sequences were considered for pre-diction to find new miRNAs.As for the degradome...

Báo cáo khoa học: " Characterisation of immune responses and protective efficacy in mice after immunisation with Rift Valley Fever virus cDNA constructs" pot

... vac-cination and infection, eight animals were vaccinated with cDNA encoding N, four with cDNA containing theopen reading frame of the GN/GC poly-protein and two groups, each containing four ... immunisation with cDNAencoding the GN/GC proteins or the GN protein but only two out of four after vaccination with cDNA encoding theGC protein, as detected by IFA performed on infected ... results indicate that vaccination with the GN/GC construct resulted in higher virus neutralisingantibody titers than the use of cDNA encoding for theindividual glycoproteins.Challenge of gene- gun...

Báo cáo khoa hoc:" Exclusion of PINK1 as candidate gene for the late-onset form of Parkinson''''s disease in two European populations" pps

... absence of muta-tions in our cohorts is the lack of influence of the PINK1 gene in the pathogenesis of late-onset PD. Individual tag-ging SNPs and tag-defined haplotypes in 500 PD patientslikewise ... of the Parkin gene. Here we report of a population-based analysis of sequencevariations within the PINK1 gene. The German cohortincludes 85 patients suffering from late-onset form of PDand ... associations with PD [14]. Futureinvestigations should include screening of potential pro-moter as well as enhancer/silencer regions of the gene tofinally exclude any lack of influence of PINK1 variation...

Tài liệu Báo cáo khoa học: Induction of uPA gene expression by the blockage of E-cadherin via Src- and Shc-dependent Erk signaling docx

... sepa-rately with Decma supernatant (Decma) for 30 min, with 3 lM CytD for 30 min, with 3 lM CytD for 45 min followed by Decma for 30 min, or simultaneously with Decma and CytD for 30 min(boxed), ... subse-quently for 4 h with Decma supernatant (b-c, e-f, h-i, k-l) before recording (a-f) or before immunostaining with the anti-E-cadherin IgG (g-l).S. Kleiner et al. Loss of E-cadherin function induces ... permea-bilized with 0.5% Triton X-100 in NaCl ⁄ Pi for 10 min,blocked with 5% normal goat serum for 20 min, incubated with anti-E-cadherin IgG (1 : 200) for 2 h, washed threetimes with NaCl ⁄ Pi, incubated...

Tài liệu Báo cáo khoa học: Characterization of the promoter for the mouse a3 integrin gene Involvement of the Ets-family of transcription factors in the promoter activity doc

... thea3b1 integrin functions as a high-afﬁnity receptor for isoforms of laminin, i.e. laminin-5 and laminin-10/11 [6–9]. More recently, thrombospondin-1 has been reported tobe a ligand for a3b1integrin[10].Thea3 ... MyoD/E-box binding factors.The introduction of mutation in one of the Ets-bindingsequences greatly decreased its promoter activity, suggestingthat the transcription of the a3 integrin gene in these ... Sciences, University of Tokyo, JapanThe a3b1 integrin is an adhesion receptor for extracellularmatrix proteins including isoforms of laminin, and thechanges of its expression level in various cancer...

Báo cáo khoa học: Control of mammalian gene expression by amino acids, especially glutamine potx

... decreased binding of the activating protein 1(AP-1) on the promoter of the endothelin-1 gene wasobserved following homocysteine addition in endothe-lial cells, resulting in inhibition of endothelin-1 ... representation of the in uence of glutamine on the transcription of genes involved in intermediarymetabolism.Table 5. In uence of glutamine on transcription factors involved in cell proliferation, ... kinases and increasedNF-jB bindingHuman endothelial cells [75]Increased CREB binding HepG2 cells [76]Increased AP-1 binding Rat hepatocytes [77]Arginine addition Increased AP-1(c-jun) binding...

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