báo cáo khoa học:" Microvascular engineering in perfusion culture: immunohistochemistry and CLSM findings" ppt

... of vas- cularization in tissue engineering and has the advantage of pointing to a solution for the problem of nutrition and oxygenation in the in vitro phase of engineering larger tis- sue equivalents. ... (see scheme in Fig. 1). During this time, the perfusion rate was raised from 100 µl/min to 500 µl/min and the pulse rate altered from 6/min to 16/min in 8 steps (every...

Ngày tải lên: 11/08/2014, 23:22

... species in red grow both on rich and on synthetic minimal medium within 7 days; species in blue grow on rich med- ium within 7 days and on synthetic minimal medium enriched with lysine and glutamic acid ... EC (1969) Inhibitors around the antimycin-sensitive site in the respiratory chain. In Inhibitors ) Tools in Cell Research (Bucher Th & Sies H, eds), pp. 282–300. Spring...

Ngày tải lên: 19/02/2014, 02:20

... P i released min )1 Æmg )1 of protein. Enzyme kinetic study The K m (Michaelis constant) and V max (maximum velocity) values were determined by incubating the diluted recombin- ant proteins in the standard ... deparaffinized and dehydrated using a graded series of alcohol and then rehydrated in NaCl/P i . The slides were blocked for 30 min in 1% H 2 O 2 in NaCl/P i containing 10%...

Ngày tải lên: 20/02/2014, 11:20

... bootstrapping. It includes co-training (Blum and Mitchell, 1998; Collins and Singer, 1998; Nigam and Ghani, 2000) and bilingual bootstrapping (Li and Li, 2002). Collaborative bootstrapping begins ... unlabeled training and test data sets. We used 3 texts per class as la- belled instances for training, 994 texts for testing, and the remaining 1000 texts as unlabelled in- s...

Ngày tải lên: 20/02/2014, 16:20

... E.N. (2001) Protein folding: looping from hydrophobic nuclei. Proteins 45, 3 46–350. 12. Berezovsky, I.N. (2003) Discrete structure of van der Waals domains in globular proteins. Protein E ngineering 16, ... a/b 2ilk Interleukin-10 a 1cbs Cellular retinoic- acid-binding protein b 1asu Retroviral integrase, catalytic domain a/b 1rro Oncomodulin a 1ivpA 2 (HIV-2) protease b 1lbbA Glutamate re...

Ngày tải lên: 07/03/2014, 16:20

... lmolÆmg of protein )1 Æmin )1 and 0.06 lmolÆmg of protein )1 Æmin )1 , respectively, while the rates were 0.05 lmolÆmg of protein )1 Æmin )1 and 0.03 lmolÆmg of protein )1 Æmin )1 when these ... contrary, no growth was observed in a culture medium containing maltose, and no acetate and succinate were produced. In addition, when cells were first incubated with maltose and then sub...

Ngày tải lên: 07/03/2014, 17:20

... motif [20]; and finally the C-terminal domain is rich in arginin and glycin residues and is able to interact with ribo- somal proteins [21] or ligands like lactoferrin [13]. Interestingly, the ... domain is involved in the interactions of surface nucleolin with most of its ligands, the RBD- containing central domain is involved in the protein self-interactions. Interestingly, preli...

Ngày tải lên: 14/03/2014, 23:20

... for informa- tion retrieval, that is, browsing and retrieving arti- cles in TV newscasts and newspapers. As a result, using the results of the alignment process, we devel- oped a browsing and ... articles in the following TV newscasts and newspapers: • NHK evening TV newscast, and • Asahi newspaper (distributed in the Internet). We used 143 articles of the evening TV n...

Ngày tải lên: 17/03/2014, 07:20

... were washed off and bound preproteins eluted with 250 mm imidazole containing buffer. In every case, precursor protein was detected in the imid- azole eluate, indicating a direct interaction with Toc34DTM. ... of ATP- and time- demands for import of Tic22, MGD1 and SSU. Import into intact pea chloroplasts was performed under standard conditions, by incubating in vitro synthesized...

Ngày tải lên: 23/03/2014, 07:20

... and suggests inefficiency of the inspection system. The main factors that intensify illegal wildlife trading in Vietnam include high domestic and international demand for wildlife meat and ... wildlife increases two to three times. - Use education and information campaigns to influence the wildlife eating and drinking culture of the Vietnamese people. In the long run,...

Ngày tải lên: 06/08/2014, 19:20