Báo cáo y học: " Influenza C virus NS1 protein counteracts RIG-I-mediated IFN signalling" potx

... This cytoplasmic localisation may reflect RIG-I antagonising properties of NS1 -C. To elucidate whether NS1 from influenza C virus also counteracts the cellular IFN response, we examined the effect ... Open Access Influenza C virus NS1 protein counteracts RIG-I-mediated IFN signalling Karin Pachler, Reinhard Vlasak * Abstract The nonstructural proteins 1 (NS1) f...

Ngày tải lên: 11/08/2014, 21:22

... including IFN mRNA by binding to CPSF30 (cleavage and polyadenylation specificity factor 30) [18] and PABPNI (poly-A-binding protein nuclear I) [19]. NS1 also directly binds the IFN effectors PKR and ... undetectable by ELISA in these infected mouse lung extracts. No virus was detected in the liver of any of the mice indicating that the viruses are incapable of growing systemically...

Ngày tải lên: 11/08/2014, 21:21

... accumulation within the hepatocyte. HCV core protein has been studied at length in both cell cultures and in transgenic mice. Intracellular lipid buildup seems to occur when the HCV core protein ... directly injure the liver but it rather triggers an HCV-specific lymphoproliferation. Through profuse cytokine production and also a direct cytopathic effect, these T cells result in he...

Ngày tải lên: 02/11/2012, 09:51

... reaction (PCR), “real-time” PCR or TMA [5]. HCV RNA is extracted and reverse transcribed into a double stranded complementary DNA (cDNA), which is subsequently processed into a cyclic enzymatic ... epidemiology studies, where exact subtyping is needed. In clinical practice, HCV genotype can be determined by various commercial kits, using direct sequence analysis of the 5’ noncoding reg...

Ngày tải lên: 02/11/2012, 09:56

... Ahmed 3 Abstract Hepatitis C virus (HCV) is the leading cause of chronic hepatitis worldwide and its subtypes/genotypes are clinically important for clinical management and vaccine development. The present study describes ... W, Kim CW, Jang JW, Bae SH, Choi JY, Jang SK, Yoon SK: Hepatitis C virus (HCV) genotyping by annealing reverse transcription- PCR products with genotype-specific...

Ngày tải lên: 11/08/2014, 21:21

... peer review • No space constraints or color figure charges • Immediate publication on acceptance • Inclusion in PubMed, CAS, Scopus and Google Scholar • Research which is freely available for redistribution Submit ... HCV, Biokit, Barcelona, Spain) and submitted to RNA detection by a nested PCR with primers complementary to the con- served area of the 5′ NC region of HCV [14]. A line probeassa...

Ngày tải lên: 12/08/2014, 04:20

... chronic HCV infectionFigure 3 The specificity of anti-HCV antibody responses in patients suffering from chronic HCV infection. A. The fre- quency of antibodies against individual recombinant HCV proteins ... the cases, while usually the infection becomes chronic. Within 20 to 30 years chronic HCV infection can progress to cirrhosis in 20% of the patients leading to hepatocellular carcinoma ro...

Ngày tải lên: 12/08/2014, 04:21

... reneewy@hku.hk; Wendy CL Yu - h0894037@hkusua.hku.hk; Carol CC Ho - chui2ho@hku.hk; WH Chui - winghungchui@yahoo.com; CK Lo - cheukkinlo@yahoo.com.hk; Kit M Yuen - kmy74@hkucc.hku.hk; Yi Guan - yguan@hkucc.hku.hk; ... cells and lung microvascular endothelial cells Michael CW Chan* †1 , Renee WY Chan †1,2 , Wendy CL Yu 1 , Carol CC Ho 1 , WH Chui 3 , CK Lo 4 , Kit M Yuen 1,2 , Yi Guan 1 , J...

Ngày tải lên: 12/08/2014, 14:20

... Biomolecular Sciences Committee PhD studentship from the Biotechnology and Biological Sciences Research Council. Research in the MH laboratory is supported by the Wellcome Trust, Medical Research ... affinity to that exhibited by the Nef:SH3 domain interaction. We conclude that NS5A binds to SH3 domains with high affinity, and such interactions could occur in the context of an HCV infected...

Ngày tải lên: 20/06/2014, 01:20

... replicatio n by IFN- a,indi- cating MxA may be independent of inhibition of HBV DNA replication by IFN- a. The clinical significance of the inhibitory effect on MxA gene transcription by HBc protein ... impact on inhibition of HBV replication by IFN- a in Huh7 cells. The clinical significance of the inhibitory effect of MxA gene transcription by HBc protein requires further study. Intr...

Ngày tải lên: 12/08/2014, 01:22