Báo cáo y học: " Recombinant luciferase-expressing human cytomegalovirus (CMV) for evaluation of CMV inhibitors" pps

... development of critical quality control assays, both in terms of their sensitivity to the samples at the point at which they are assayed, as well as their utility as a predictor of sequen- cing quality. ... handling Precision performance of the liquid handling robot is maintained by regular quality control. A dummy run is performed daily in which 5 μl of a 0.1-M solution of sulfor...

Ngày tải lên: 09/08/2014, 22:23

... cytometry for CD4 + T lymphocyte depletion and cell death analysis All flow cytometry was performed with a FACS Calibur with BD CellQuest Pro 4.0.2 Software (BD Pharmingen). For the analysis of ... 1 Department of Virology, University of Heidelberg, Heidelberg, Germany and 2 Department of Otolaryngology, Head and Neck Surgery, University of Heidelberg, Heidelberg, Germany E...

Ngày tải lên: 13/08/2014, 05:21

... Borstel, Department of Medicine, Borstel, Germany. 2. Department of Medicine I, University Hospital Hamburg-Eppendorf, Germany. 3. Department of Microbiology and Immunology, University Hospital Hamburg-Eppendorf, ... and mortality in the majority of CF patients (1). This pathogen colonises the airways and lungs often in the late teens or early twenties and can be controlled for...

Ngày tải lên: 03/11/2012, 11:48

... Prevention (CDC) calls for increased level of activity by incorporating any activity of at least moderate intensity into the day. For older adults, the daily accumu- lation of PA (stair climbing, ... likely to relapse after 10 months, particularly if they remain physically active during the follow-up period [32]. Finally, the results from the Almada County Study showed that physical...

Ngày tải lên: 08/08/2014, 20:23

... project beyond the surrounding DNA. In the case of H3 and H4, specific lysine sidechains undergo acetylation, through the action of histone acetyltransferases, by way of acetyl coenzyme A, a step which ... histone deacetylase inhibitors trichostatin A and butyrate were shown to drastically reduce expression of these enzymes relatively selectively, with concomitant inhibition of brea...

Ngày tải lên: 09/08/2014, 06:23

... be upregulated by hypoxia, which is also the case for many cytokines that can be found in myositis muscle. Recently, we found that polymyositis and dermatomyositis patients with a short duration of symptoms ... characteristic of inclusion body myositis, which is clinically different from polymyositis and dermatomyositis by slowly progressive weakness of proximal leg and distal arm mus...

Ngày tải lên: 09/08/2014, 13:21

... phagocyte stimula- tion. First, the peptides prevent activation of NF-κB by a variety of inflammatory stimuli (reviewed in [4]). Therefore, they may inhibit MSU crystal-induced secretion of chemoattractants and ... (MSU)-stimulated human monocytesEffect of the caspase-1 inhibitor z-YVAD-fmk on inflammatory mediator production by monosodium urate (MSU)-stimulated human monocytes....

Ngày tải lên: 09/08/2014, 14:22

... any automation. Settings used for plate-based shearing of DNA are: Duty Cycle of 5; Intensity of 5; Cycle per Burst of 500; Seconds of 120; Well Plate of '96 well offset + 5 mm'. It is ... immobiliza- tion (SPRI) of DNA. Binding of nucleic acids to carboxyl- para-magnetic microparticles can be made selective for molecular weight by manipulating concentrations of...

Ngày tải lên: 09/08/2014, 20:21

... followed by pheno typic analysis. In general, the availability of com- pletely sequenced genomes of a variety of organisms has increased the popularity of this approach, because know ledge of the ... cardiovascular disease, by selective breeding for naturally occurring disease pheno- types [4]. One of the main advantages of using the rat for studying human biology is...

Ngày tải lên: 09/08/2014, 22:23

... Users can use any affymetrix array for which they have the appropriate library files, but for the following arrays those files can be automatically downloaded and installed by EP-manage.pl:HG-U133(A/B),HG- U133_Plus_2, ... part of another project, and maybe even have been performed on another plat- form (for example, RNA-Seq versus microarray) or in another organism (for example, hum...

Ngày tải lên: 09/08/2014, 23:20