Báo cáo y học: "HIV-1 Nef increases astrocyte sensitivity towards exogenous hydrogen peroxide" potx

... viability of human astrocytes exposed to these particular cytotoxic substances. Results Astrocytes stably transfected with HIV-1 nef are highly sensitive to hydrogen peroxide induced cell death Astrocytes ... how this is modulated by HIV-1 Nef. Therefore, the sensitivity of human astrocytic U251MG -Nef cells towards hydrogen peroxide was tested in comparison with the sensitivity...

Ngày tải lên: 11/08/2014, 21:21

... the onset of symptoms signifi- cantly reduced bone erosions as detected by x-ray analysis (Figure 4a–c). No difference was found when treatment was started on day 35, presumably due to the fact ... detected significantly reduced overall cellularity, as shown by conven- tional histology, and a trend toward reduced granulocyte infil- tration. However, we cannot exclude the possibility that othe...

Ngày tải lên: 09/08/2014, 08:22

... levels in mouse 3T3-L1 adi- pocytes [49,50]. Recently, it was demonstrated that tea catechins directly bind to DNA and preferentially bind to poly(A), poly(U), and poly(AU) in RNA [42]. It will be interesting ... HW, Matschinsky FM, Stanley CA, Smith TJ: Green tea polyphenols modulate insulin secretion by inhibiting glutamate dehydro- genase. J Biol Chem 2006, 281:10214-10221. 42. Kuzuhara T,...

Ngày tải lên: 11/08/2014, 08:21

... hypertonic saline may afford some protection by increasing GSH in CFTR KO airways, this protective response is limited by t he inability to elevate their already deficient ELF SCN levels. Hypertonic ... increase ELF GSH with hypertonic saline exposure. This would indicate that an yone with CF, independent of the type of mutation, may benefit from hypertonic saline treatment yet those who stil...

Ngày tải lên: 12/08/2014, 11:22

... airways may grad- ually induce airway smooth muscle hypertrophy and hyperplasia. Moreover, our finding that TGF-β1 enhances serum-induced ASMC proliferation may occur in asth- matic airways where ... with 0.05% trypsin/0.53 mM EDTA. Smooth muscle cell identity was verified by phase contrast micro- scopy for appearance of "hill and valley formation" and by immunocytochemistry stainin...

Ngày tải lên: 12/08/2014, 16:20

... homozy- gosity [7]. Interestingly, the virus has evolved a strategy to selectively down-regulate HLA-A and -B but not HLA-C, via the regulatory protein Nef [8]. The immunity of HLA- C to Nef- mediated ... glycoprotein gp120 and enhance viral infectivity. We investigated this last property in cell fusion assays where the expression of HLA-C was silenced by small interfering RNA sequences. S...

Ngày tải lên: 13/08/2014, 05:21

... monophosphate). Subjects heterozygous for this variant allele appear to h ave a 50% reduction in enzyme activity [33]. Interestingly, heterozygosity was recently associated with an improved cardiovascular ... addition, in septic shock patients, not only the inflammatory response but also tissue hypoperfusio n may play a role in the formation of adenosine. We subsequently aimed to demonstrate...

Ngày tải lên: 14/08/2014, 07:21

... 1,6-bisphosphatase aldolase 4 GlyA Serine hydroxymethyltransferase 5 LpdA* (AtpA) Lipoamide dehydrogenase (F1 ATP synthase a subunit) 6 Ppa* (AhpC) Inorganic pyrophosphatase (alkyl hydroperoxide reductase ... proteins, cells were lysed in lysis buffer containing 0.2 mgÆmL )1 lysozyme, 0.2 m M phenylmethanesulfonyl fluoride and 1 m M dithiothreitol, followed by sonication. The cell lysates were...

Ngày tải lên: 22/02/2014, 04:20

... telomerase activity was measured by TRAP/EIA cells 3 days later. (C) Telomerase activity after introduction of antisense oligonucleotides into PHA- stimulated lymphocytes. Ficoll-Hypaque isolated lymphocytes ... TEP1 function by high concentra- tions of antisense oligonucleotides, telomerase activity was dramatically diminished. Alternatively, hTR and hTERT may play a minimal catalytic activi...

Ngày tải lên: 17/03/2014, 23:20

... dimeric prototypes for the respective families of c-andb-crystallins, andsimplyreferredtoasc-type or b-type crystallins, respectively. Both monomeric c-type crystallin and the subunit of dimeric b-type crystallin ... bB2-crystallin (PDB 1BLB), the dimeric b–type prototype, and (B) cB-crystallin (PDB 4GRC). The monomeric c-type prototype. Fragments of cB-crystallin are shown to illustrate sche...

Ngày tải lên: 24/03/2014, 04:21