Báo cáo y học: " Inhibition of lung serine proteases in mice: a potentially new approach to control influenza infection" doc

... thereby initiate the infection process [1-4]. The cleavage site of HA0 of most avian and mam- malian influen za viruses is monobasic and carries a sin- glearginine,rarelyasinglelysineaminoacid.Cleavage has ... Switzerland). This substrate contains an alkaline amino acid (arginine) in theP1siteupstreamthep-NA group that favors proteolysis by trypsin like serine proteases at alkalin...

Ngày tải lên: 11/08/2014, 21:21

... mechanisms. Background Respiratory syncytial virus (RSV), a leading cause of epi- demic respiratory tract infection in infants, spreads prima- rily by contact with contaminated secretions and replicates in the nasopharyngeal ... WD, Yang H, Kong X, Mohapatra S, Juan-Vergara HS, Heller- mann G, Behera S, Singam R, Lockey RF, Mohapatra SS: Inhibition of respiratory syncytial virus i...

Ngày tải lên: 20/06/2014, 01:20

... nonmannosylated GPtdIns glucosamine-phosphatidylinositol (GlcN–PtdIns) and glu- cosamine-acylphosphatidylinositol (GlcN-acyl–PtdIns) (Fig. 2). These data imply that mannosamine was incorporated into ... nor nonmannosylated intermediates accumulated in the pre- sence of mannosamine. The absence of new mannosamine- containing GPtdIns and the inhibition of incorporation of tritiate...

Ngày tải lên: 31/03/2014, 23:20

... Aggarwal BB: Protein farnesyltransferase inhibitor (SCH 66336) abolishes NF-kappaB activation induced by various carcinogens and inflammatory stimuli leading to suppression of NF-kappaB-regulated ... [26] as well as pro-inflammatory cytokines such as Ccl2, Il6, and Ifn β [27] induced by carcinogens and inflammatory stimuli. We have shown here that clinically-relevant nanomolar doses of...

Ngày tải lên: 11/08/2014, 08:21

... gelatinases A and B, stromelysin, and the cathepsins. Extracellular degradation of collagen can be incomplete, leaving fragments to be phagocytosed by cells such as fibroblasts, macrophages and ... humans. By activat- ing granzymes A and B, DPPI might regulate the lymphocyte-mediated cytotoxicity of virally infected or malignant cells [45]. Cytotoxic T lymphocytes and natural killer...

Ngày tải lên: 12/08/2014, 18:20

... function as a major HIV-1 reser- voir in vivo. In addition, monocytes are programmed to produce a large amount of inflammatory cytokine, includ- Genome structure and expression of recombinant pNL(AD8)IκB-αS32/3 6A ... levels of transcription factors, includ- ing NF-κB, which sustain the initial rounds of viral repli- cation and induce the production of inflammatory cytoki...

Ngày tải lên: 13/08/2014, 13:20

... release of pro-inflammatory cytokines by circulating monocytes and may explain, at least in part, the failure of most of these trials. Down-regulation of cytokine production was accom- panied by ... impaired in their capacity to produce these pro-inflammatory cytokines in vitro [3]. This impairment is part of a second hypo- inflammatory state of the septic cascade also k...

Ngày tải lên: 14/08/2014, 07:21

... ERK1/2. Recent observations indicate that activation of Raf by PMA may trigger the same signaling pathway as oncogenic Raf, or Raf activation by Ras in combination with tyrosine phosphorylation [30]. ... of different intracellular checkpoint pathways. Among these, the mitogen-activated protein kinase (MAPK)/extracellular signal-regulated kinase (ERK) pathway has been proposed to play...

Ngày tải lên: 21/02/2014, 01:21

... lus ¯anking exons of genomic rabbit DNA was ampli®ed by PCR techniques. For PCR, the primers b-glb1/ P(5¢-AATCTGGTACCTCACCTGGACAACCTCAAA- 3¢)andb-glb2/M (5¢-AATAAGAATTCGCCAAAAT GATGAGACAGCA-3¢) ... self-cleavage (tetracycline), of group-I intron self- splicing (streptomycin) a nd inhibition of RNase P cleavage by some am inoglycosides. Cl-tetracycline a nd the amino- cyclitol glyc...

Ngày tải lên: 21/02/2014, 15:20

... (Millipore Japan, Tokyo, Japan), and stained with anti-spHAS antibody according to the method of Towbin et al.[35]using3,3¢-diaminobenzidine tetrahydrochloride (Dojindo Laboratories, Kumamoto, Japan) ... 25–1190 of AF023876. RNA preparations and Northern hybridization Total RNA was prepared from FM100 cells using a RNeasy Plant Mini Kit (Qiagen Japan, Tokyo, Japan) according to the...

Ngày tải lên: 08/03/2014, 09:20